External Support

• Marika Ziesack, M.sc.''' for giving us an introduction to Gibson Cloning
• Matthew Mattozzi, Ph.D.''' from the Harvard Wyss Institute for Biologically Inspired Engineering, Center for Life Sciences in Boston who contributed helpful advice on genomic homologous recombination. Dr. Mattozzi also kindly provided the pET21c-pcc-acc-mmce plasmid.
• Prof. Dr. Herzig''' and his group at the German Cancer Research Center, Heidelberg for offering the possibility to use their electroporator.
• PD Dirk M. Nettelbeck, Ph.D.''' and his group at the German Cancer Research Center for his input on homologous recombination.
• Lei Fang from the Pfeifer Lab''' at the Department of Chemical and Biological Engineering, University of Buffalo, for providing the BAP1 strain and the pLF03 and pKD46 plasmids.
• the '''Gruss Lab''' at the Center for Molecular Biology at the University Heidelberg who kindly provided us with the BL21(DE3) (pLys :-)) strain.
• Prof. Russel''''s group for supporting us with a vial of real BL21(DE3) cells.
• Prof. Dr. Marahiel''' at the Philipps-University Marburg, for providing us with the ''Brevibacillus parabrevis'' ATCC 8185 strain.
• Prof. Dr. Thomas Höfer''''s Group at the BioQuant, Heidelberg for providing a sample of ''E.coli'' BL21 pLys.
• Prof. Fussenegger''' for providing us with the expression plasmids for bpsA and svp, codon optimized for mammalian cells
• Prof. Groß''' at the mass spectrometry facility (institute of organic chemistry, University of Heidelberg) for providing his HR-ESI MS.
• Dr. Oliver Wichmann''' at the BioQuant, Heidelberg for supporting us with materials including his lyophilizer.
• Dr. Dirk Kohlmüller''' and '''Dr. Kathrin Schmidt''' at the neonate screening, University Medical Center Heidelberg for their support in amino acid level detection by mass spectrometry.
• Dr. Holger Schäfer''' from the Institute of Pharmacology and Molecular Biotechnology for scientific and moral support during cloning of Del constructs.
• Prof. Dr. Klein''''s lab at the Institute of Pharmacology and Molecular Biotechnology for giving us the possibility to use their Micro-TOF and especially '''Lena Weigel''' for her great support.
• And special thanks to Thi Bach '''Nga Ly-Hartig''' who put us in touch with several research groups that work with mass spectrometry and bringing us cupcakes