Team:NTU-Taida/Notebook/Journal
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Contents |
July
7/1~7/5
Finish RhlR and LasR circuit.
7/1
Transform:
- Positive feedback circuit:
Pc-A-C-B, Pc-A-C-E, Pc-A-C-B, Pc-D-F-B, Pc-D-F-E, Pc-D-F-G; - Control:
PLas-B, PLas-E, PLas-G, PRhl-B, PRhl-E, PRhl-G;
(A: RBS-RhlR-tt C: PRhl-RBS-RhlR D: RBS-LasR-tt F: PLas-RBS-LasR B: RBS-mCherry-tt E: RBS-GFPmut-tt G: RBS-mRFP-tt )
- New:
Pcin, CinR (resistant=C)
7/2
Result:
There’s no colonies at plates of Pcin and CinR.
Transform:
Pcin(resistant=A), CinR (resistant=K)
Inoculation and incubation at LB broth
- Positive feedback circuit: Pc-A-C-B, Pc-A-C-E, Pc-A-C-B, Pc-D-F-B, Pc-D-F-E, Pc-D-F-G;
- Control: PLas-B, PLas-E, PLas-G, PRhl-B, PRhl-E, PRhl-G; (A: RBS-RhlR-tt C: PRhl-RBS-RhlR D: RBS-LasR-tt F: PLas-RBS-LasR B: RBS-mCherry-tt E: RBS-GFPmut-tt G: RBS-mRFP-tt )
7/3
Inoculation and incubation at LB broth
Pcin(resistant=A), CinR (resistant=K)
Check
- Positive feedback circuit: Pc-A-C-B, Pc-A-C-E, Pc-A-C-B, Pc-D-F-B, Pc-D-F-E, Pc-D-F-G;
- Control: PLas-B, PLas-E, PLas-G, PRhl-B, PRhl-E, PRhl-G; (A: RBS-RhlR-tt C: PRhl-RBS-RhlR D: RBS-LasR-tt F: PLas-RBS-LasR B: RBS-mCherry-tt E: RBS-GFPmut-tt G: RBS-mRFP-tt )
7/4
Check and Plasmid DNA extraction (mini-prep):
- Positive feedback circuit: Pc-A-C-B, Pc-A-C-E, Pc-A-C-B, Pc-D-F-B, Pc-D-F-E, Pc-D-F-G;
- Control: PLas-B, PLas-E, PLas-G, PRhl-B, PRhl-E, PRhl-G; (A: RBS-RhlR-tt C: PRhl-RBS-RhlR D: RBS-LasR-tt F: PLas-RBS-LasR B: RBS-mCherry-tt E: RBS-GFPmut-tt G: RBS-mRFP-tt )
- Pcin(resistant=A), CinR (resistant=K)
7/5
Check: (for plasmid ready to be sequenced)
ACB-4, ACE-5, ACG-1, DFB-1, DFE-2, DFE-3, DFE-4, DFE-5, DFG-1, RhlB-1, RhlE-2, RhlG-1, RhlG-2, LasB3, LasE1, LasG4 (8,9,11 well failed :DFE-4/DFE-5/ RhlB-1)
7/8~7/12
7/8
7/9
7/10
7/11
7/12
7/15~7/19
7/15
7/16
7/17
7/18
7/19
7/22~7/31
7/22
7/23
7/24
7/25
7/26
7/27
7/28
7/29
7/30
7/31
July
2