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Team:ETH Zurich/Experiments
From 2013.igem.org
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<p>For the final Colisweeper circuit we plan a four plasmid system. The mine cells constitutively express LuxI for signal generation and NagZ as identifier hydrolase. In the non-mine cells LuxR is expressed constitutively to process the OHHL signal. PhoA is expressed constitutively as well as reporter for safe cells. Aes and GusA are expressed from pLux promoters with different sensitivities. You can find all the biobricks we used and our own new biobricks in the figure below.</p> | <p>For the final Colisweeper circuit we plan a four plasmid system. The mine cells constitutively express LuxI for signal generation and NagZ as identifier hydrolase. In the non-mine cells LuxR is expressed constitutively to process the OHHL signal. PhoA is expressed constitutively as well as reporter for safe cells. Aes and GusA are expressed from pLux promoters with different sensitivities. You can find all the biobricks we used and our own new biobricks in the figure below.</p> | ||
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| - | <img src="https://static.igem.org/mediawiki/2013 | + | |
| - | <map id="map3" name="map3" > <area shape="rect" alt="" title="NagZ: BBa_K1216003" coords="259,77,340, | + | <img src="https://static.igem.org/mediawiki/2013/b/b7/Plasmidmap.png" usemap="#map3" id="imagemap3"> |
| + | <map id="map3" name="map3" > <area shape="rect" alt="" title="NagZ: BBa_K1216003" coords="259,77,340,113" href="http://parts.igem.org/Part:BBa_K1216003" target="" /><area shape="poly" alt="" title="constitutive promoter: BBa_J23100" coords="161,85,221,85,221,72,245,96,221,121,219,107,157,108" href="http://parts.igem.org/Part:BBa_J23100" target="" /><area shape="rect" alt="" title="LuxI: BBa_K805016" coords="255,229,328,265" href="http://parts.igem.org/Part:BBa_K805016" target="" /><area shape="poly" alt="" title="constitutive promoter: BBa_J23110" coords="158,245,216,245,217,231,241,258,217,277,218,267,157,268" href="http://parts.igem.org/Part:BBa_J23110" target="" /><area shape="rect" alt="" title="LuxR: BBa_I0462" coords="682,76,763,114" href="http://parts.igem.org/Part:BBa_I0462" target="" /><area shape="poly" alt="" title="promoter" coords="589,103,651,96,647,82,674,105,655,130,653,117,593,127" href="" target="" /><area shape="poly" alt="" title="LuxR: BBa_I0462" coords="880,85,962,96,958,131,876,120" href="http://parts.igem.org/Part:BBa_I0462" target="" /><area shape="poly" alt="" title="constitutive promoter: BBa_J23100" coords="782,83,843,88,842,76,864,101,839,123,839,111,778,108" href="http://parts.igem.org/Part:BBa_J23100" target="" /><area shape="rect" alt="" title="Aes: BBa_K1216002" coords="674,236,737,273" href="http://parts.igem.org/Part:BBa_K1216002" target="" /><area shape="poly" alt="" title="Lux promoter with low sensitivity: BBa_K1216007" coords="580,262,638,253,638,241,664,263,646,289,643,275,584,285" href="http://parts.igem.org/Part:BBa_K1216007" target="" /><area shape="rect" alt="" title="GusA: BBa_K1216000" coords="871,243,951,278" href="http://parts.igem.org/Part:BBa_K1216000" target="" /><area shape="poly" alt="" title="Lux promoter with high sensitivity: BBa_R0062" coords="769,246,831,247,829,233,851,258,828,283,828,268,766,267" href="http://parts.igem.org/Part:BBa_R0062" target="" /><!-- Created by Online Image Map Editor (http://www.maschek.hu/imagemap/index) --></map> | ||
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| + | <b>Figure 1. Plasmids in mine and non-mine cells: move the cursor over the separate parts to check which biobricks we used.</b> | ||
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Revision as of 12:26, 24 September 2013
Final Circuit
For the final Colisweeper circuit we plan a four plasmid system. The mine cells constitutively express LuxI for signal generation and NagZ as identifier hydrolase. In the non-mine cells LuxR is expressed constitutively to process the OHHL signal. PhoA is expressed constitutively as well as reporter for safe cells. Aes and GusA are expressed from pLux promoters with different sensitivities. You can find all the biobricks we used and our own new biobricks in the figure below.
Figure 1. Plasmids in mine and non-mine cells: move the cursor over the separate parts to check which biobricks we used.