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Team:Heidelberg/NRPS
From 2013.igem.org
Project
Notebook
Human Practice
Delftibactin.Recycling Gold from Electronic Waste.
Highlights
- Production and purification of delftibactin, a gold-precipitating NRP, from its native, cultured host Delftia acidovorans.
- Recovery of pure gold from electronic waste by Delftia acidovorans and purified delftibactin.
- Optimization of the Gibson assembly method for the creation of large plasmids (> 30 kbp) with high GC content.
- Amplification and cloning of all components required for recombinant delftibactin production.
- Transfer of the entire pathway from D. acidovorans for the synthesis of delftibactin to E. coli.
Abstract
Efficient recycling of gold from electronic waste using recombinant delftibactin
Undoubtedly, gold is one of the most precious materials on earth. Besides its common use in art and jewelry, gold is also an essential component of our modern computers and cell-phones. Due to the fast turn-over of today’s high-tech equipment, millions of tons of electronic waste accumulate each year containing tons of this valuable metal. The main approach nowadays to recycle gold from electronic waste is by electrolysis. Unfortunately, this is a highly inefficient and expensive procedure, preventing most of the gold from being recovered.
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