Team:Heidelberg/Project/Achievements

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        <title>Achievments</title>
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                         <h1 style="float:left"><span style="font-size:150%;color:#333333;">Achievements.</span><span class="text-muted" style="font-size:85%"> Introducing NRPS to Synthetic Biology.</span></h1>
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                         <h1><span style="font-size:150%;color:#FFCC00;">iGEM Medals</span><span class="text-muted" style="font-size:90%"> for Non-Software Teams</span></h1>  
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                            <div id="bronzemedal" class="medal" style="text-align: center";><h8><b>Bronze Medal </b> Requirements</h8>
 
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                                        <p>Team is registered!  <span class="btn btn-default btn-lg medal-requirement glyphicon glyphicon-hand-right" style="font-size: 11px;" data-trigger='click', data-title="iGEM Team Heidelberg", data-content='The Team Heidelberg is finally back in the iGEM competition this year. If you want to know more about our team visit our team-page! Link zum Team-Page'>
 
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                                        <p> Judging form is completed and submitted!  <span href="#" class="btn btn-default btn-lg medal-requirement glyphicon glyphicon-hand-right" style="font-size: 11px;" data-trigger='click', data-title="Judging form", data-content='Our judging form was filled in and is available online.'> </p></span>
 
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                                        <p> Project description of the Philosopher's Stone online! <span href="#" class="btn btn-default btn-lg medal-requirement glyphicon glyphicon-hand-right" style="font-size: 11px;" data-trigger='click', data-title="Project description", data-content='Numerous non-ribosomal peptides such as antibiotics, chelators of metals, dyes and detoxificating enzymes are produced in an alternative ribosome-independent pathway as secondary metabolites, found in fungi and various bacteria. The chain of modules the enzyme - called non-ribosomal peptide synthetase (NRPS) - consists of, subsequently adds a defined amino acid to build a template-independent peptide chain. Every module’s sequence of subdomains furthermore encodes for possible chemical modifications of the amino acid. <br/><br/>We will investigate modularity of the NRPS systems giving rise to the assembly of novel, engineered synthetases and subsequent synthesis of artificial non-ribosomal peptides. Currently, we are establishing the introduction of different NRPS pathways into E. coli. Our aim is to prove interchangeability of NRPS modules and domains from different host organisms. Smaller peptide synthetases, e.g. producing dyes, are used for the exchange of domains, whereas more complex synthetases, e.g. producing antibiotics, were chosen to investigate module exchangeability.<br/><br/> We wish to share the enormous potential of non-ribosomal peptide synthetases with the iGEM community by establishing a standard system for these synthetases, supported by an implemented software framework. Thereby, we are going to simplify manufacturing and applicability of non-ribosomal peptides in order to offer a cost- and energy-efficient alternative for the synthesis of biomolecules. As secondary metabolites, these peptides play important roles in basic research (such as dyes), pharmaceutical development (such as antibiotics) and recycling (such as chelators). '>
 
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                                        <p> Poster is prepared for the European Jamboree in Lyon!  <span href="#" class="btn btn-default btn-lg medal-requirement glyphicon glyphicon-hand-right"style="font-size: 11px;" data-trigger='click', data-title="Poster presentation at European jamboree", data-content='We are looking forward to see you at the poster presentation!'>
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<a class="fancybox fancyGraphical" href="https://static.igem.org/mediawiki/2013/3/3e/Heidelberg_Achievements_sidebar.png">
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                                        <p> New standard BioBrick is documented! <span href="#" class="btn btn-default btn-lg medal-requirement glyphicon glyphicon-hand-right" style="font-size: 11px;" data-trigger='click', data-title="New standard Biobrick", data-content='We did not only add one, but 20 parts to the registry. Feel free to browse the parts we offer the community. Link zur partsseite....'>
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    <img style="width:100%; margin-top:2%; padding:1%;border-style:solid;border-width:0px;border-radius: 5px;" src="https://static.igem.org/mediawiki/2013/3/3e/Heidelberg_Achievements_sidebar.png" ></img>
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Automated Design of <b>Custom Non-Ribosomal Peptide Synthetases</b>
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Production of <b><a href="https://2013.igem.org/Team:Heidelberg/Project/Tyrocidine">Synthetic Peptides</a></b> in different engineered <em>E. coli</em> Hosts
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Invention of a <a href="https://2013.igem.org/Team:Heidelberg/Project/Indigoidine-Tag">Universal Tag</a></b> for Non-Ribosomal Peptide labeling <em>in vivo</em>
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Using the <a href="https://2013.igem.org/Team:Heidelberg/Project/Indigoidine-Tag">Tag</a> for <b>peptide characterization and quantification of production</b>
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Application of <a href="https://2013.igem.org/Team:Heidelberg/Project/Delftibactin">delftibactin NRP</a> for <b>sustainable Gold Recycling</b>
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<br><br><br>
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Introducing our standardized Framework for Production of Synthetic Peptides to the Synbio Community: <b><a href="https://2013.igem.org/Team:Heidelberg/RFCs">RFC 99 and RFC 100</a></b>
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<b>Having lots of <a href="<a href="https://2013.igem.org/Team:Heidelberg/Team/Gallery">fun</a> and very much looking forward to the Jamboree in Boston</b>
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                                <div id="silvermedal" class="medal" style="text-align: center";><h8><b>Silver Medal </b> Requirements
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                    <p> New BioBricks are characterized! <span  href="#" class="btn btn-default btn-lg medal-requirement glyphicon glyphicon-hand-right" style="font-size: 11px;"data-trigger='click', data-title="New characterized Biobricks", data-content='We characterized our new BioBricks experimentally and documented our findings in the parts registry: Link zu partsregistry'></p></span><br/>
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                    <p> New BioBricks are submitted! <span href="#" class="btn btn-default btn-lg medal-requirement glyphicon glyphicon-hand-right" style="font-size: 11px;"data-trigger='click', data-title="New submitted Biobricks", data-content='We submitted 20 parts to the parts registry - ranging from the basis for a NRPS-module library, to novel NRPSs and constructs, where entirely synthetic protein-domains were introduced. Link zur parts seite'></p></span><br/>
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                        <h1 style="float:left"><span style="font-size:150%;color:#333333;">Medal Requirements.</span><span class="text-muted" style="font-size:85%"> Aiming for Gold.</span></h1>
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                    <p> Implications for ethics are pointed out! <span href="#" class="btn btn-default btn-lg medal-requirement glyphicon glyphicon-hand-right" style="font-size: 11px;"data-trigger='click', data-title="Project`s implications", data-content='At the beginning of the project, we pointed out possible ethical implications in an essay. (bei essay)Link zum Essay von Philipp. '></p></span>
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            <h8><b>Best New Standard</b></h8>
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                    <span class="requirement">We submitted RFC99 and RFC100!</span>
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                    <span  class="btn btn-default btn-lg medal-requirement glyphicon glyphicon-hand-right" style="font-size: 11px;;" data-title="New standard - RFC" data-placement="right" data-content='We developed a standardized framework for the production of synthetic peptides based on engineered NRPSs. Our framework is documented in &lt;a href="/Team:Heidelberg/RFCs#rfc99"  target="_blank"&gt;&lt;b&gt;RFC 99&lt;/b&gt;&lt;/a&gt; and &lt;a href="/Team:Heidelberg/RFCs" &gt;&lt;b&gt;RFC 100&lt;/b&gt;&lt;/a&gt;!'></span>
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                <a href="/Team:Heidelberg/RFCs#rfc99"><span class="highlight_button" style="background-image: url('https://static.igem.org/mediawiki/2013/0/01/Heidelberg_bn_round_rfc99.png');"></span></a>
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                <a href="/Team:Heidelberg/RFCs"><span class="highlight_button" style="background-image: url('https://static.igem.org/mediawiki/2013/7/7f/Heidelberg_bn_round_rfc100.png');"></span></a>
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            <h8 ><b>Parts!</b></h8>
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                     <span class="requirement">Have a look on our different parts!</span>
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            <div id="goldmedal" class="medal" style="text-align:center" ><h8><b>Gold Medal </b> Requirements</h8><br/>
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                    <span  class="btn btn-default btn-lg medal-requirement glyphicon glyphicon-hand-right" style="font-size: 11px;" data-trigger='click' data-placement="bottom"  data-title="Bringing Non-Ribosomal Peptide Synthesis to iGEM- our Parts" data-content='&lt;a href="https://2013.igem.org/Team:Heidelberg/Parts"&gt;&lt;b&gt;Visit our parts page!&lt;/b&gt;&lt;/a&gt;'></span>
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                     <p> Functions of existing BioBricks were improved! <span   href="#" class="btn btn-default btn-lg medal-requirement glyphicon glyphicon-hand-right" style="font-size: 11px;"data-trigger='click', data-title="Improved BioBricks", data-content='Functions of three existing BioBricks were improved: <br/> <br/> 1) We annotated the PPTase on [Part, link zur registry]. By submitting additional PPTases to the parts registry, we expanded apllications of PPTases. <br/> <br/> 2) We identified a missing sequence in the backbone pSB4K5 from the spring distribution of 2012. <br/><br/> 3) We further characterized the toxicity of [Part, link zur registry], an exporter in <i>E. coli</i>. '></p> </span><br/>
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            <h8><b>Attribution and Contribution!</b></h8>
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                    <p> We helped other iGEM teams! <span  href="#" class="btn btn-default btn-lg medal-requirement glyphicon glyphicon-hand-right" style="font-size: 11px;"data-trigger='click', data-title="Collaboration", data-content='In the past months, we collaborated with other student iGEM teams: We sent Freiburg a eukaryotic cell line. Norwich received soil samples from the beautiful forests of Heidelberg and we helped Purdue by participating in their survey. Additionally, we fixed a bug in Cambridge's Gibthon software tool. The parts registry is now properly connected to Gibthon and importing parts directly from the registry is possible (if they accept our pull request on GitHub).'></p> </span><br/>
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                    <p> Novel approach to ownership and sharing is described!<span class="btn btn-default btn-lg medal-requirement glyphicon glyphicon-hand-right" style="font-size: 11px;"data-trigger='click', data-title="NRPS-Designer", data-content='With the NRPS Designer we share with the iGEM community is a novel approach to ownership and sharing in synthetic biology. Visit our NRPS Designer and try it yourself.'></p> </span> <br/><br/><br/><br/><br/><br/><br/><br/><br/><br/>
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                    <span class="requirement">Thank you for the great help!</span>
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                    <span class="btn btn-default btn-lg medal-requirement glyphicon glyphicon-hand-right" style="font-size: 11px;" data-trigger='click' data-placement="left"  data-title="Attributions, Sponsors and Acknowledgements"  data-content='We thank our &lt;a href="https://2013.igem.org/Team:Heidelberg/Sponsors"&gt;&lt;b&gt; sponsors&lt;/b&gt;&lt;/a&gt;, &lt;a href="https://2013.igem.org/Team:Heidelberg/Team/Acknoledgements"&gt;&lt;b&gt;all people&lt;/b&gt;&lt;/a&gt; that kindly shared their laboratory equipment and constructs with us and &lt;a href="https://2013.igem.org/Team:Heidelberg/AttributionsII"&gt;&lt;b&gt;attribute&lt;/b&gt;&lt;/a&gt; the work of our team!'></span>
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                <div id="best" class="medal"><h8><b>Best New Standard</b></h8>
 
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            <p>  Improve the function of an existing BioBrick Part or Device <span  class="btn btn-default btn-lg medal-requirement glyphicon glyphicon-hand-right" style="font-size: 11px;margin-right:60%;"data-trigger='click', data-title="Non-ribosomal peptide synthetase", data-content='Please explain briefly why you should receive any of these special prizes:'></p> </span>
 
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                <div id="parts" class="medal" ><h8 ><b>Parts!</b></h8>
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                 <p>Have a look on our different parts! <span class="btn btn-default btn-lg medal-requirement glyphicon glyphicon-hand-right" style="font-size: 11px;margin-right:60%;"data-trigger='click', data-title="Non-ribosomal peptide synthetase", data-content='Link to our parts? '></p> </span>
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        <div id="bronzemedal" class="medal">
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            <h8><b>Bronze Medal</b> Requirements</h8>
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            <div>
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                    <span class="check"></span>
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                    <span class="requirement">Team is registered!</span>
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                    <span class="btn btn-default btn-lg medal-requirement glyphicon glyphicon-hand-right" style="font-size: 11px;" data-title="iGEM Team Heidelberg" data-content='The Team Heidelberg is finally back in the iGEM competition this year. If you want to know more about our team visit our &lt;a href="https://2013.igem.org/Team:Heidelberg/Team"&gt;&lt;b&gt;team-page&lt;/b&gt;&lt;/a&gt;!'></span>
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                    <span class="check"></span>
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                    <span class="requirement">Judging form is completed and submitted!</span>
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                    <span class="btn btn-default btn-lg medal-requirement glyphicon glyphicon-hand-right" style="font-size: 11px;" data-title="Judging form" data-content='Our &lt;a href="https://igem.org/2013_Judging_Form?id=1152"&gt;&lt;b&gt;judging form&lt;/b&gt;&lt;/a&gt; was filled in and is available online.'></span>
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                    <span class="check"></span>
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                    <span class="requirement">Project description of the Philosopher's Stone online!</span>
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                    <span class="btn btn-default btn-lg medal-requirement glyphicon glyphicon-hand-right" style="font-size: 11px;" data-title="Project description" data-content='Our project description is available on the &lt;a href="https://2013.igem.org/Team:Heidelberg/Project"&gt;&lt;b&gt;Project website&lt;/b&gt; &lt;/a&gt; were you can also get a basic overview.'></span>
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                    <span class="requirement">Poster is prepared for the European Jamboree in Lyon!</span>
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                    <span class="btn btn-default btn-lg medal-requirement glyphicon glyphicon-hand-right" style="font-size: 11px;" data-title="Poster presentation at European jamboree" data-content='Yupi! We won the prize for the best poster!!!'></span>
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                    <span class="check"></span>
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                    <span class="requirement">New standard BioBrick is documented!</span>
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                    <span class="btn btn-default btn-lg medal-requirement glyphicon glyphicon-hand-right" style="font-size: 11px;" data-title="New standard Biobrick" data-content='We submitted 20 BioBricks which are summarized on &lt;a href="https://2013.igem.org/Team:Heidelberg/Parts"&gt;&lt;b&gt;submitted parts.&lt;/b&gt;&lt;/a&gt; Feel also free to browse the parts we offer the community, for example &lt;a href="http://parts.igem.org/Part:BBa_K1152013?title=Part:BBa_K1152013"&gt;&lt;b&gt;BBa_K1152013&lt;/b&gt;&lt;/a&gt;'></span>
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            <h8><b>Silver Medal</b> Requirements</h8>
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                    <span class="check"></span>
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                    <span class="requirement">New BioBricks are characterized!</span>
 +
                    <span class="btn btn-default btn-lg medal-requirement glyphicon glyphicon-hand-right" style="font-size: 11px;"  data-title="New characterized Biobricks"  data-placement="bottom" data-content='We characterized our new BioBricks experimentally and documented our findings in the parts registry: for example: &lt;a href="http://parts.igem.org/Part:BBa_K1152006?title=Part:BBa_K1152006"&gt;&lt;b&gt;BBa_K1152006&lt;/b&gt;&lt;/a&gt;'></span>
 +
                </div>
 +
                <div class="requirement">
 +
                    <span class="check"></span>
 +
                    <span class="requirement">New BioBricks are submitted!</span>
 +
                    <span class="btn btn-default btn-lg medal-requirement glyphicon glyphicon-hand-right" style="font-size: 11px;" data-placement="bottom"  data-title="New submitted Biobricks" data-content='We submitted &lt;a href="https://2013.igem.org/Team:Heidelberg/Parts"&gt;&lt;b&gt;20 well characterized parts&lt;/b&gt;&lt;/a&gt; to the parts registry - ranging from the basic NRPS-modules and custom NRPSs producing peptides of choice to constructs for NRP tagging and NRPS constructs, where entirely synthetic NRPS-domains were introduced.'></span>
 +
                </div>
 +
                <div class="requirement">
 +
                    <span class="check"></span>
 +
                    <span class="requirement">Implications for ethics are pointed out!</span>
 +
                    <span class="btn btn-default btn-lg medal-requirement glyphicon glyphicon-hand-right" style="font-size: 11px;" data-title="Project's implications" data-placement="bottom" data-content='At the beginning of the project, we pointed out possible ethical implications in an essay. You can read this essay here: &lt;a href="https://2013.igem.org/Team:Heidelberg/Ethics"&gt;&lt;b&gt;Essay!&lt;/b&gt;&lt;/a&gt;'></span>
 +
                </div>
 +
            </div>
 +
        </div>
 +
            <div id="goldmedal" class="medal">
 +
            <h8><b>Gold Medal</b> Requirements</h8>
 +
            <div>
 +
                <div class="requirement">
 +
                    <span class="check"></span>
 +
                    <span class="requirement">Functions of existing BioBrick was improved!</span>
 +
                    <span class="btn btn-default btn-lg medal-requirement glyphicon glyphicon-hand-right" style="font-size: 11px;" data-title="Improved BioBricks" data-placement="left" data-content='The function of the BioBricks &lt;a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K802006"&gt;&lt;b&gt;BBa_K802006&lt;b&gt;&lt;/a&gt; and &lt;a href="http://parts.igem.org/Part:BBa_K302010"&gt;&lt;b&gt;BBa_K302010&lt;b&gt;&lt;/a&gt; were improved with our &lt;a href="http://parts.igem.org/Part:BBa_K1152009?title=Part:BBa_K1152009"&gt;&lt;b&gt;BBa_K1152009&lt;/b&gt;&lt;/a&gt;'></span>
 +
                </div>
 +
                <div class="requirement">
 +
                    <span class="check"></span>
 +
                    <span class="requirement">We helped other iGEM teams!</span>
 +
                    <span class="btn btn-default btn-lg medal-requirement glyphicon glyphicon-hand-right" style="font-size: 11px;" data-title="Collaboration" data-placement="left" data-content="In the past months, we &lt;a href='https://2013.igem.org/Team:Heidelberg/Team/Collaboration'&gt;&lt;b&gt;collaborated&lt;/b&gt;&lt;/a&gt; with other student iGEM teams: We sent Freiburg a eukaryotic cell line. Norwich received soil samples from the beautiful forests of Heidelberg and we helped Purdue by participating in their survey. Additionally, we fixed a bug in Cambridge's Gibthon software tool. The parts registry is now properly connected to Gibthon and importing parts directly from the registry is possible (if they accept our pull request on GitHub)."></span>
 +
                </div>
 +
                <div class="requirement">
 +
                    <span class="check"></span>
 +
                    <span class="requirement">Novel approach to ownership and sharing as well as safety is described!</span>
 +
                    <span  class="btn btn-default btn-lg medal-requirement glyphicon glyphicon-hand-right" style="font-size: 11px;" data-title="NRPS-Designer" data-placement="left"  data-content="The &lt;a href='http://igem2013.bioquant.uni-heidelberg.de/NRPSDesigner/'&gt;&lt;b&gt;NRPSDesigner&lt;/b&gt;&lt;/a&gt; software is our way of sharing our knowledge about NRPS engineering with the iGEM community. During the development and programming of the NRPSDesigner we employed different &lt;a href='https://2013.igem.org/Team:Heidelberg/OpenSource'&gt;&lt;b&gt;open source&lt;/b&gt;&lt;/a&gt; software packages and also improved some of those in order to support the open source programming community. Please feel free to visit our &lt;a href='https://2013.igem.org/Team:Heidelberg/Safety'&gt;&lt;b&gt;Safety page&lt;/b&gt;&lt;/a&gt; also."></span>
 +
                </div>
 +
            </div>
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        </div>
 +
    </div>
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                  <div id="attribution" class="medal"><h8><b>Attribution and Contribution!</b></h8>
 
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                <p>  Thank you for the great help! <span class="btn btn-default btn-lg medal-requirement glyphicon glyphicon-hand-right" style="font-size: 11px; margin-right:60%;" data-trigger='click', data-title="...", data-content='Link to sponsors and other donors of stuff and time... '></p></span>
 
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Latest revision as of 03:52, 29 October 2013

Achievements. Introducing NRPS to Synthetic Biology.


Automated Design of Custom Non-Ribosomal Peptide Synthetases


Production of Synthetic Peptides in different engineered E. coli Hosts


Invention of a Universal Tag for Non-Ribosomal Peptide labeling in vivo
Using the Tag for peptide characterization and quantification of production


Application of delftibactin NRP for sustainable Gold Recycling


Introducing our standardized Framework for Production of Synthetic Peptides to the Synbio Community: RFC 99 and RFC 100
Having lots of fun and very much looking forward to the Jamboree in Boston

Medal Requirements. Aiming for Gold.



Best New Standard
We submitted RFC99 and RFC100!
Parts!
Have a look on our different parts!
Attribution and Contribution!
Thank you for the great help!
Bronze Medal Requirements
Team is registered!
Judging form is completed and submitted!
Project description of the Philosopher's Stone online!
Poster is prepared for the European Jamboree in Lyon!
New standard BioBrick is documented!
Silver Medal Requirements
New BioBricks are characterized!
New BioBricks are submitted!
Implications for ethics are pointed out!
Gold Medal Requirements
Functions of existing BioBrick was improved!
We helped other iGEM teams!
Novel approach to ownership and sharing as well as safety is described!
Thanks to

Retrieved from "http://2013.igem.org/Team:Heidelberg/Project/Achievements"