Team:Heidelberg/Project/Indigoidine-Tag

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Indigoidine-Tag. Introducing the GFP for NRPs.

Highlights

  • Creation of an easily detectable, inert and universal NRPS-Tag using the Indigoidine Synthetase
  • easy verification of Non-Ribosomal Peptides via Thin Layer Chromatography
  • Proof of principle module shuffling and domain-import among species
  • Establishment of a standardized protocol for High-Throughput NRPS-assays: RFC 100 and RFC 99

Abstract

Synthesizing peptides with various functions using Non-Ribosomal Peptide Syntheatses (NRPS) provides access to more than 500 building blocks, and hence opens up an unimaginably diverse spectrum of possible products. However, detection of the Non-Ribosomal Peptide (NRP), as well as high-throughput screening of peptide functionality remained complicated or even impossible. What would be required is an easily detectable, inert and universal tag that allows simplified screening and detection, similar to the GFP-tag for proteins.

Indigoidine, a blue pigment which is synthesized by the NRPS IndC from Photorhabdus luminescens laumondii TT01 (DSM15139), was established as a novel and application-oriented tag functionally fused to synthesized peptides. Streamlining this process demonstrates a substantial reinforcement of the NRPS-Designer, our software tool, and offers the possibility to evaluate and optimize synthesis of Non-Ribosomal Peptides (NRPs) in a high-throughput manner (RFC 99 & RFC 100).

Introduction

Non-Ribosomal Peptide Synthetases (NRPS) are assembly lines which consist of several modules, each module incorporating one specific amino acid into the growing peptide chain (for a more detailled introduction into NRPS, please see our Background Page). As previously shown by both other research groups and our work (Project Peptide-Synthesis), NRPS modules can be rearranged to form a novel assembly line which produces a custom non-ribosomal peptide [15]. However, the detection of those synhetic peptides using mass spectrometry is still challenging. In order to simplify the detection of peptides created by custom NRPSs, we developped a tag for non-ribosomal peptides - the Indigoidine-Tag. The indigoidine synthetase indC from Photorhabdus luminescens laumondii TT01 (DSM15139) is a single module NRPS, which catalyzes the production of an insoluble blue pigment called indigoidine from two L-glutamines (Fig. 1a)[12]. This leads to a blue phenotype of E. coli cells expressing the indC gene when grown on plates or in liquid cultures (Fig. 1b). The blue pigment can be purified and dissolved in DMSO using a simple protocol.

Figure 1: The Indigoidine Synthetase IndC Catalyzes the Formation of the Blue Pigment Indigoidine.
a) The indigoidine synthetase indC from P. luminescens is a single module NRPS catalyzing the formation of the blue pigment indigoidine by cyclization and oxidation of two L-glutamines.
b) Expression of a functional indigoidine synthetase in E. coli BAP1 cells leads to a blue phenotype.


We fused the indC gene to NRPS modules of the tyrocidine synthesis cluster from Brevibacillus parabrevis to create novel NRPS assembly lines, which attach the blue pigment indigoidine to the last amino acid of the synthesized peptide (Fig. 2). After purification, the tagged peptide can be validated using Comparative Thin Layer Chromatography or Mass-Spectrometry after purification using High Pressure Liquid Chromatography.

Figure 2: The IndC Module is Fused to Other NRPS Modules to Establish the Indigoidine-Tag.
When combining the coding sequences of NRPS modules from diverse Non-Ribosomal Peptide Synthetases, such as antibiotic biosynthesis clusters, with the indC indigoidine synthetase module, the resulting assembly line will eventually produce a indigidine-tagged peptide.

The possibility of tagging non-ribosomal peptides makes high-throughput protocols possible. Therefore, we standardized the whole process from designing novel NRPSs with our NRPS Designer software, followed by high-throughput construction of NRPS libraries and the detection and validation of the synthetic peptides up to functional assays and possible upscaling of the peptide production to industrial level, in our RFC100).


Results

Showing inter-species module compatibility by fusion of Tyrocidine modules to the Indigoidine synthetase

Our module-shuffling approach within the Tyrocidine cluster was confirmed by mass spectrometry. Access to such special technical devices and the referring expertise is demanded for detection of small peptides but often limited. That is why we were thinking of a potential alternative to test for the synthesis of NRPs. We wanted to establish an assay accessible to the majority of the community for validation of the presence of custom peptides. Since the synthetase for indigoidine consists of only a single module, it could serve as a paradigm for the fusion to modules of other NRPSs. The pigment Indigoidine could potentially ease detection of peptides when they are fused to the dye that is visible by eye.


Figure 3: Schematic flow of the idea behind the NRP-tag via Indigoidine. As Mass-Spectrometry is very effortful and sometimes inconclusive, a tag for NRPs is required. This tag should have the following characteristics: Easy to detect, inert, small and universal. We investigated the dimerized pigment Indigoidine as it fulfills those requirements.

Thus we wanted to investigate whether it is possible to fuse Indigoidine as a tag to NRPs of other pathways even originating from other species. In the following, we will showcase the extent of NRPSs’ modular compatibility by creating inter-species module-fusions between the Indigoidine synthetase from P. luminescens and modules of the Tyrocidine synthetase from B. parabrevis. In those fusion NRPs, Indigoidine serves as a tag that eases identification of E. coli clones synthesizing the customized peptide.

Fusing single amino acids to Indigoidine

First, we combined single modules of the Tyrocidine synthetase with the indC synthetase resulting in three distinct NRPSs producing Asparagin, Valine or Phenylalanine respectively, all tagged with Indigoidine (see Fig. 4).


Figure 4: Composition of three fusion constructs originating from Tyrocidine (tyc) synthetase modules and the Indigoidine synthetase (indC). First row indicates domains with referring modules. Coloured modules in the three rows below were fused together to create plasmids encoding novel NRPSs (rectangle).

To assure compatibility the constructs were designed in such a way that the C domain of the C2 module was always used, given its specificity for Glutamine required for the Indigoidine production. SDS-PAGE showed the expected bands for the expression of the NRP synthetases in the transformed BAPI. The E. coli strain BAPI was used for expression of the NRPS fusions because it carries the required PPTase endogenously. All three of the fusion variants turned the colonies blue even before expression induction with IPTG. The blue pigment thus served a first indicator that peptide synthesis was successful. To further verify the existence of the fusion peptide, we ran comparative thin-layer chromatography (TLC). The native, purified Indigoidine ran further than our purified dipeptides suggesting that the amino acids were indeed fused to the pigment (Fig. 5). The peptides were detected under visible and UV light due to Indigoidine’s properties as a dye.


Figure 5: Comparative TLC of our tagged NRP Val-Ind with native Indigoidine. Three different biological replicates of the produced NRP, a Valine-Indigoidine fusion peptide (Val-Ind), are compared to an Indigoidine control (Ind-ctrl). Clearly visibly, the produced NRP shows a significantly altered migration behavior on Thin Layer Chromatography with Dichloromethane as running solvent.

Using Indigoidine as tag for non-ribosomal peptides

To gather additional evidence for our functional Indigoidine tag, we assembled seven variants with up to three modules in front of the Indigoidine synthetase (Fig. 6) following the same approach as described above.


Figure 6: Composition of seven fusion constructs originating from Tyrocidine (tyc) synthetase modules and the Indigoidine synthetase (indC). Those contructs serve as a proof of principle for the tagging of Non-Ribosomal Peptides with Indigoidine. Several constructs were created using a Valine-spacer in order to assess the influence of sterically hindrance of bigger or polar amino acids. To be able to show the applicability of the tag most clearly, the Di- and Tripeptide constructs described on the Peptide Synthesis page, were tagged with Indigoidine as well.

Again the constructs pPW06, pPW09, pPW10, pPW11 and pPW12 turned BAPI colonies blue upon transformation. We tested the fusion of those peptides by comparative TLC with native Indigoidine. Even with increasing peptide length, synthesis did not seem to be affected and the dye-properties of the Indigoidine were still preserved (Fig. 7).


Figure 7: Comparative TLC of different tagged NRPs with native Indigoidine. Indigoidine (Ind-ctrl), for this purpose, serves as negative control to show the significantly altered migration behavior of longer peptides containing Indigoidine. We hence show that Thin Layer Chromatography can be used as standardizes, easily realizable validation of the produced constructs. In this case, migration behavior of Valine-Indigoidine (Val-Ind), Asparagine-Indigoidine (Asn-Ind), Ornithine-Valine-Indigoidine (Orn-Val-Ind) and Phenylalanine-Ornithine-Leucine-Indigoidine (Tri-Ind) was assessed.

From this, we deduced that Indigoidine possesses characteristics required for a proper peptide tag that we would like to propose for use to the community (RFC 100). For this purpose we have created a ccdB-dependent vector to ease the tagging of NRPs, accessible through the parts registry. Design of such constructs is enabled with our software, the NRPS Designer. The ccdB-helper-construct (BBa_K1152007) is designed to lower the background of negative transformants: If the insert is cloned in the vecor correctly, there is no active ccdB present and the cell survives. If the backbone religates or template-backbone is still present in the Gibson-Mix, cells will die due to ccdB. The clearly visible impact of ccdB is visible in Fig. 8


Figure 8: Effect of ccdB on non-resistant cells. regular TOP10 cells die in presence of active ccdB (left side), while ccdB-resistant cells survive (right side). Hence this comparison shows the effectiveness of ccdB and the minimization of background to about 0%. Using the ccdB-helper plasmid for a Gibson-driven tagging-approach enhances effectiveness significantly.

Experimental validation of software predictions

We propose the Indigoidine-Tag as a part of our standards RFC 99 and RFC 100. The crucial tool in this standardized process is the NRPS Designer. The NRPS Designer predicts module boundaries and linker regions based on Pfam. We used these predictions for our module shuffling experiments, which all worked well in our constructs. However, other tools predict other boarders, and hence we want to evaluate the predictions to contribute more data to the NRPS Designer and to provide experimental feedback to our software. Therefore, we systematically varied the module boundaries of the A domain and C domain of C2 within the Val-Ind-construct in relation to the Pfam prediction. Based on this altered domain positioning, eight constructs were designed combining narrow, broad and original Pfam module regions (Fig. 9). Their functionality was always compared to the original construct based on the boundaries obtained from Pfam. We could induce the production of Val-Ind in two of our constructs: pLV03 (narrow and Pfam border) and pLV08 (broad and narrow border)


Figure 9: Overview of our constructs for investigation of the different domain borders in relation to Pfam. Dark colors are variations in the start-region of the A domain borders and light colors are variations in the end-region of the C domain. Setting of proper domain boarders is crucial for module shuffling, both, within and in between pathways and organisms. We hence assessed adequate definitions of where and how to build the linker regions by vaying them and checking for functionality. We therefore created the eight constructs shown above. Those are all Valine-Indigoidine synthetases with different settings of domain boarders.

To summarize, with our inner- and interspecies module shuffling, we successfully validated the concept of modularity. We have integrated our experimental data and fuelled it into our software to improve its prediction accuracy. To make NRPSs and their custom design more accessible to the community, we have submitted standardized versions of the modules we used for our shuffling experiments, to the parts registry (see the parts registry entries).


Discussion

The bottleneck to test libraries of combinatorial NRPSs with an array of different modules is in fact the screening for functional enzymes [13] [14]. How to identify novel NRPSs consisting of compatible modules? Our experimental results (Fig. 5) strongly support the hypothesis that the synthesis of short peptides can be easily monitored when fused to Indigoidine. This novel technique that represents an equivalence of the GFP-tag for proteins in NRPs outrules classical detection by Mass-Spec due to the increased efficiency and high-throughput character. Our findings that NRPSs seem to offer a framework going beyond species borders confirm the results shown by Marahiel [15]. Using Indigoidine as pigment-module for the fusion results in a fusion NRP which is even detectable by eye.

With this, we offer a novel and very efficient way of tagging NRPs with Indigoidine. The dye can be easily measured, quantified and even optimized. This aspect furthermore appends a valuable feature of the NRPS Designer. Users now have the opportunity to easily add an Indigoidine tag when they design their constructs, which is also described in our proposed RFC (RFC 100). Noteworthy, our experiments we could show that the Indigoidine tag works fine when put behind modules for different amino acids, but the tagging clearly works best, when an apolar, small spacer amino-acid such as Glycine, Alanine or, as in our experiments, Valine is used, due to less steric hindrance.


Figure 10: Predicted tertiary structure of the Valine-Indigoidine-Synthetase. Crucial domains are clearly visible and distinguishable in prediction. Prediction was carried out using PHYRE2 secondary structure prediction with multiple subsets of the protein sequence.

Compared with other potential methods for in-vivo tagging of NRPs, as it has been described before [16] and [17], the Indigoidine tag has the apparent advantage that it is relatively small compared to e.g. a Haemagglutinine tag. Similarly, using fluorescent proteins (FPs) as tag is hardly feasible as the peptides that should be tagged are often smaller than the chromophore of those FPs. Imagining e.g. GFP synthesized by an NRPS is practically not feasible. Something similar accounts for other tags, such as the His tag, for which four to nine Histidines are required. Synthesizing a short peptide with several modules for Histidine is imaginable, but would double or triple the size of the required NRPS, and hence of the vector, which is highly ineffective. In this context, the Indigoidine tag would be especially suited for analytical sample labeling. In summary, the Indigoidine-Tag, in contrast to all other imaginable tagging methods for NRPs fulfills the required characteristics of being small, inert, universal and easily detectable.

As far as in-vitro approaches are concerned, there are, in principle, two ways. I) Either one could add a tagging agent to the cells or the medium before purification – which would be the case for e.g. Click-Chemistry. II) Or one could add a certain tag such as a His tag to the NRPS and perform the entire synthesis of the NRP in vitro. The latter approach has been widely used [18] [19], is, however in vitro and hence less effective for a high-throughput advance as a functioning in-vivo-method. Besides, the upscaling of such an approach would hardly be feasible. We ourselves thought of using methods such as Click-Chemistry for the purification of the short, synthetic peptides. Increased masses and easy cleavage enable high purities and can be determined via HPLC with UV and ESI-MS detection [20]. This approach, however, does not offer any opportunity to evaluate expression in vivo[21].

Hence, using and Indigoidine-tag, which can be added to the nascent NRP in the process of its formation by adding a 4 kbp Indigoidine-module to the NRPS is a novel and effective approach for labeling NRPs for quantitative expression analysis. Its properties and effects for peptide synthesis via NRPS compare to the ones of GFP for proteins: it is relatively inert, easily detectable and universal


Methods

Purification of Indigoidine and tagged constructs

1 ml of IPTG-induced, blue culture was spun down at full speed (14,000 rpm) for 20 minutes, washed in 1 ml of methanol and centrifuged once more for 5 minutes at 14,000 rpm. Methanol was discarded and samples were dissolved in 200-400 µl DMSO.


Figure 11: Workflow followed during purification of the tagged peptides. A: Blue overnight culture with the secreted tagged peptide to purify. B: Blue pellet after spinning down cells for 20 minutes at 14,000 rpm. C: The pellet is washed in methanol and spun down again. D: The tagged peptide dissolves in DMSO and can be applied to TLC or further purified if wished.

Thin Layer Chromatography

TLC was carried out on silica-gel as immobile phase and Dichloromethane as mobile phase. For the procedure, a 50 ml beaker was filled with ~15 ml Dichloromethane and let stand for about 10 minutes (in order to let the Dichloromethane-vapor fill the beaker). The TLC plate, coated with silica-gel was spotted with sample 0.5 to 1 cm above the lower edge and placed in the beaker. TLC was run until the solvent front was at two thirds of the TLC plate. As Indigoidine is light-sensitive, the beaker was covered with aluminium foil in order to prevent direct light irradiation.

Column Chromatography

In order to show that further purification (beyond the regular purification procedure) is feasible and easy with the tagged peptides, we provide a protocol for analysis and purification of the sample via column-chromatography. For this purpose, the purified sample that is dissolved in DMSO is applied to a ..., as running buffer, DMSO was used. DMSO is constantly applied to the column to prevent it from drying. All uncolored fractions are discarded, while the blue fraction is kept and collected in a 2 ml Eppendorf tube. This purified extract (with the sample dissolved in DMSO) can then be used for TLC or Mass-Spectrometry if desired.


Figure 12: Column Chromatography of tagged peptides. A: The blue fraction can be easily detected and collected. B: The purified product is dissolved and DMSO and can then be further analyzed.

RFC 100

For the entire practical procedure of the lab-work that is involved in our standardized protocol for high-throughput cloning and fast purification and validation of the product, feel welcome to visit the RFC 100-page.


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