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Team:NTU-Taida/Notebook/Functional Assay
From 2013.igem.org
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#Overview of functional assays set up | #Overview of functional assays set up | ||
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Function of BBa_K575033 measured by flow cytometry | Function of BBa_K575033 measured by flow cytometry | ||
| - | = | + | ==9.12-9.25== |
#QS molecule composition of conditioned media of clinical isolates detected by our constructs and old biobricks, including those for Rhl, Lux, Las and Pqs. | #QS molecule composition of conditioned media of clinical isolates detected by our constructs and old biobricks, including those for Rhl, Lux, Las and Pqs. | ||
#Helped Team NYMU to do some functional assays | #Helped Team NYMU to do some functional assays | ||
| - | = | + | ==9.26== |
Function of Rhl circuits (Normal, PFL, CI) were measured by flow cytometry | Function of Rhl circuits (Normal, PFL, CI) were measured by flow cytometry | ||
Latest revision as of 21:12, 27 September 2013
Functional assay journal
Contents |
Functional assay journal
7.23
- Overview of functional assays set up
- ELISA reader tutorial
7.30
Rhl (C4-AHL) sensor with positive feedback-loop tested but failed. The problems may resulted from
- Incomplete dissolution of C4-AHL chemicals. Adjust the pH to the optimal.
- Insensitivity of the construct itself
8.5
Found out that store the microtiter plate inside a plastic bag and cover it with aluminum foil would minimize the evaporation of the organic solvent, making the concentration more stable.
8.16
Signals from Rhl (C4-AHL) sensor were barely detected. SDS-PAGE was performed to do some semi-quantitative trouble-shooting. We contact team NYMU for help.
8.20
The function of basic Rhl sensor was assayed. Seems it worked.
9.2
Contact Dr. Hsueh for obtaining clinical isolates for further experiments. AHL quantal-dose and temporal responses completed.
9.6
Function of BBa_K575033 measured by flow cytometry
9.12-9.25
- QS molecule composition of conditioned media of clinical isolates detected by our constructs and old biobricks, including those for Rhl, Lux, Las and Pqs.
- Helped Team NYMU to do some functional assays
9.26
Function of Rhl circuits (Normal, PFL, CI) were measured by flow cytometry
