"
Team:NTU-Taida/Notebook/Functional Assay
From 2013.igem.org
(→Functional assay logbook) |
|||
| Line 2: | Line 2: | ||
=Functional assay logbook= | =Functional assay logbook= | ||
| - | == | + | ==7.23== |
#Overview of functional assays set up | #Overview of functional assays set up | ||
| - | # ELISA reader tutorial | + | #ELISA reader tutorial |
| - | == | + | ==7.30== |
Rhl (C4-AHL) sensor with positive feedback-loop tested but failed. The problems may resulted from | Rhl (C4-AHL) sensor with positive feedback-loop tested but failed. The problems may resulted from | ||
#Incomplete dissolution of C4-AHL chemicals. Adjust the pH to the optimal. | #Incomplete dissolution of C4-AHL chemicals. Adjust the pH to the optimal. | ||
#Insensitivity of the construct itself | #Insensitivity of the construct itself | ||
| - | == | + | ==8.5== |
| - | + | Found out that store the microtiter plate inside a plastic bag and cover it with aluminum foil would minimize the evaporation of the organic solvent, making the concentration more stable. | |
| - | == | + | ==8.16== |
| - | + | Signals from Rhl (C4-AHL) sensor were barely detected. SDS-PAGE was performed to do some semi-quantitative trouble-shooting. We contact team NYMU for help. | |
| - | == | + | ==8.20== |
| - | + | The function of basic Rhl sensor was assayed. Seems it worked. | |
| - | == | + | ==9.2== |
Contact Dr. Hsueh for obtaining clinical isolates for further experiments. AHL quantal-dose and temporal responses completed. | Contact Dr. Hsueh for obtaining clinical isolates for further experiments. AHL quantal-dose and temporal responses completed. | ||
| - | == | + | ==9.6== |
Function of BBa_K575033 measured by flow cytometry | Function of BBa_K575033 measured by flow cytometry | ||
Revision as of 18:16, 27 September 2013
Functional assay journal
Contents |
Functional assay logbook
7.23
- Overview of functional assays set up
- ELISA reader tutorial
7.30
Rhl (C4-AHL) sensor with positive feedback-loop tested but failed. The problems may resulted from
- Incomplete dissolution of C4-AHL chemicals. Adjust the pH to the optimal.
- Insensitivity of the construct itself
8.5
Found out that store the microtiter plate inside a plastic bag and cover it with aluminum foil would minimize the evaporation of the organic solvent, making the concentration more stable.
8.16
Signals from Rhl (C4-AHL) sensor were barely detected. SDS-PAGE was performed to do some semi-quantitative trouble-shooting. We contact team NYMU for help.
8.20
The function of basic Rhl sensor was assayed. Seems it worked.
9.2
Contact Dr. Hsueh for obtaining clinical isolates for further experiments. AHL quantal-dose and temporal responses completed.
9.6
Function of BBa_K575033 measured by flow cytometry
2013.09.12-2013.09.25
- QS molecule composition of conditioned media of clinical isolates detected by our constructs and old biobricks, including those for Rhl, Lux, Las and Pqs.
- Helped Team NYMU to do some functional assays
2013.09.26
Function of Rhl circuits (Normal, PFL, CI) were measured by flow cytometry
