Team:NTU-Taida/Notebook/Journal

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{{:Team:NTU-Taida/Templates/Header}}{{:Team:NTU-Taida/Templates/Navbar}}{{:Team:NTU-Taida/Templates/Sidebar-calendar|Title=Journal|Month=July}}{{:Team:NTU-Taida/Templates/ContentStart}}
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{{:Team:NTU-Taida/Templates/Header}}{{:Team:NTU-Taida/Templates/Navbar}}{{:Team:NTU-Taida/Templates/ContentStart}}
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=July=
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-
 
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= 7/1~7/5 =
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Finish RhlR and LasR circuit.
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-
 
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==7/1==
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-
 
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===Transform:===
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<html>
<html>
-
<ol>
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<div id="Slide" style="width:640px;margin:auto;">
 +
<div class="page-header">
 +
<h1>Journal</h1>
 +
                        <p>Press the <b>month</b> on the picture to enter monthly Journal!</p>
 +
</div>
 +
<div id="myCarousel" class="carousel slide">
 +
            <div class="carousel-inner">
 +
                <div class="item active">
 +
                    <img src="/wiki/images/a/a0/NTU-Taida-schedule-1.jpg" width="640px" alt="">
 +
                    <div class="carousel-caption" >
 +
<h4>December</h4>
 +
<p>Recruited members and Started!</p>
 +
                    </div>
 +
                </div>
 +
                <div class="item">
 +
                    <img src="/wiki/images/c/c5/NTU-Taida-schedule-2.jpg" width="640px" alt="">
 +
                    <div class="carousel-caption">
 +
<h4>January</h4>
 +
<p>Design thinking working shop for creative ideas.</p>
 +
                    </div>
 +
                </div>
 +
                <div class="item">
 +
                    <img src="/wiki/images/e/e4/NTU-Taida-schedule-3.jpg" width="640px" alt="">
 +
                    <div class="carousel-caption">
 +
<h4>February</h4>
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<p>Primary project discussion.</p>
 +
                    </div>
 +
                </div>
 +
<div class="item">
 +
                    <img src="/wiki/images/5/5b/NTU-Taida-schedule-4.jpg" width="640px" alt="">
 +
                    <div class="carousel-caption">
 +
<h4>March</h4>
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<p>Wiki study group and discussion.</p>
 +
                    </div>
 +
                </div>
 +
<div class="item">
 +
                    <img src="/wiki/images/3/39/NTU-Taida-schedule-5.jpg" width="640px" alt="">
 +
                    <div class="carousel-caption">
 +
<h4>April</h4>
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<p>Biobrick came and wet lab started.</p>
 +
                    </div>
 +
                </div>
 +
<div class="item">
 +
                    <a href="https://2013.igem.org/Team:NTU-Taida/Notebook/Journal/May"><img src="/wiki/images/2/25/NTU-Taida-schedule-6.jpg" width="640px" alt=""></a>
 +
                    <div class="carousel-caption">
 +
<a href="https://2013.igem.org/Team:NTU-Taida/Notebook/Journal/May">May</a>
 +
<p>Visit professor in Department of Biochemical Science and Technology.</p>
 +
                    </div>
 +
                </div>
 +
<div class="item">
 +
                    <a href="https://2013.igem.org/Team:NTU-Taida/Notebook/Journal/June"><img src="/wiki/images/3/32/NTU-Taida-schedule-7.jpg" width="640px" alt=""></a>
 +
                    <div class="carousel-caption">
 +
<h4><a href="https://2013.igem.org/Team:NTU-Taida/Notebook/Journal/June">June</a></h4>
 +
<p>Walking on a busy road.</p>
 +
                    </div>
 +
                </div>
 +
<div class="item">
 +
                    <a href="https://2013.igem.org/Team:NTU-Taida/Notebook/Journal/July"><img src="/wiki/images/d/d2/NTU-Taida-schedule-8.jpg" width="640px" alt=""></a>
 +
                    <div class="carousel-caption">
 +
<h4><a href="https://2013.igem.org/Team:NTU-Taida/Notebook/Journal/July">July</a></h4>
 +
<p>Go to Department of Laboratory Medicine in NTU Hospital</p>
 +
                    </div>
 +
                </div>
 +
<div class="item">
 +
                    <a href="https://2013.igem.org/Team:NTU-Taida/Notebook/Journal/August"><img src="/wiki/images/d/d0/NTU-Taida-schedule-9.jpg" width="640px" alt=""></a>
 +
                    <div class="carousel-caption">
 +
<h4><a href="https://2013.igem.org/Team:NTU-Taida/Notebook/Journal/August">August</a></h4>
 +
<p>Seminar for IGem teams in NCTU</p>
 +
                    </div>
 +
                </div>
 +
<div class="item">
 +
                    <a href="https://2013.igem.org/Team:NTU-Taida/Notebook/Journal/September"><img src="/wiki/images/a/a7/NTU-Taida-schedule-10.jpg" width="640px" alt=""></a>
 +
                    <div class="carousel-caption">
 +
<h4><a href="https://2013.igem.org/Team:NTU-Taida/Notebook/Journal/September">September</a></h4>
 +
<p>Fighting and fighting!!!</p>
 +
                    </div>
 +
                </div>
 +
            </div>
 +
        </div> 
 +
        <div style="height: 100px">
 +
<ol class="carousel-indicators">
 +
                <li data-target="#myCarousel" data-slide-to="0" class="active">
 +
<img src="/wiki/images/a/a0/NTU-Taida-schedule-1.jpg" width="50px" alt=""></li>
 +
<li data-target="#myCarousel" data-slide-to="1">
 +
<img src="/wiki/images/c/c5/NTU-Taida-schedule-2.jpg" width="50px" alt=""></li>
 +
                <li data-target="#myCarousel" data-slide-to="2">
 +
<img src="/wiki/images/e/e4/NTU-Taida-schedule-3.jpg" width="50px" alt=""></li>
 +
<li data-target="#myCarousel" data-slide-to="3">
 +
<img src="/wiki/images/5/5b/NTU-Taida-schedule-4.jpg" width="50px" alt=""></li>
 +
<li data-target="#myCarousel" data-slide-to="4">
 +
<img src="/wiki/images/3/39/NTU-Taida-schedule-5.jpg" width="50px" alt=""></li>
 +
<li data-target="#myCarousel" data-slide-to="5">
 +
<img src="/wiki/images/2/25/NTU-Taida-schedule-6.jpg" width="50px" alt=""></li>
 +
<li data-target="#myCarousel" data-slide-to="6">
 +
<img src="/wiki/images/3/32/NTU-Taida-schedule-7.jpg" width="50px" alt=""></li>
 +
<li data-target="#myCarousel" data-slide-to="7">
 +
<img src="/wiki/images/d/d2/NTU-Taida-schedule-8.jpg" width="50px" alt=""></li>
 +
<li data-target="#myCarousel" data-slide-to="8">
 +
<img src="/wiki/images/d/d0/NTU-Taida-schedule-9.jpg" width="50px" alt=""></li>
 +
<li data-target="#myCarousel" data-slide-to="9">
 +
<img src="/wiki/images/a/a7/NTU-Taida-schedule-10.jpg" width="50px" alt=""></li>
 +
            </ol>
 +
</div>
 +
</div>
 +
 +
<!-- Le javascript
 +
    ================================================== -->
 +
    <!-- Placed at the end of the document so the pages load faster -->
 +
    <script src="http://ajax.googleapis.com/ajax/libs/jquery/1.9.1/jquery.min.js"></script>
 +
    <script src="js/jquery.smooth-scroll.min.js"></script>
 +
    <script src="js/bootstrap.min.js"></script>
 +
    <script src="js/bootswatch.js"></script>
 +
<script>//usermenu dropdown
 +
if ($('#pt-logout').length){
 +
// login
 +
$('#nav-login').text('User: ' + $('#pt-userpage').text()).append('<b class="caret"></b>');
 +
$('#nav-edit').attr('href',$('#menubar.left-menu > ul > li > a[href$="edit"]').attr('href'));
 +
$('#nav-history').attr('href',$('#menubar.left-menu > ul > li > a[href$="history"]').attr('href'));
 +
$('#nav-logout').attr('href',$('#pt-logout > a').attr('href'));
 +
$('.dropdown-toggle').dropdown();
 +
}else{
 +
// not login
 +
$('#nav-usermenu').remove();
 +
$('#nav-login').attr('href',$('#pt-login>a').attr('href')).removeAttr('data-toggle');
 +
}
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<li>Positive feedback circuit:<br>
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reactiveNavbar();
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Pc-A-C-B, Pc-A-C-E, Pc-A-C-B, Pc-D-F-B, Pc-D-F-E, Pc-D-F-G;</li>
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});
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<li>Control: <br>
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PLas-B, PLas-E, PLas-G, PRhl-B, PRhl-E, PRhl-G;</li>
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(A: RBS-RhlR-tt C: PRhl-RBS-RhlR D: RBS-LasR-tt F: PLas-RBS-LasR B: RBS-mCherry-tt E: RBS-GFPmut-tt G: RBS-mRFP-tt )
+
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<li>New: <br>
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Pcin, CinR (resistant=C)</li>
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</ol>
+
 +
</script>
 +
</html>
</html>
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==7/2==
 
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===Result:===
 
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There’s no colonies at plates of Pcin and CinR. 
 
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===Transform:===
 
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Pcin(resistant=A), CinR (resistant=K)
 
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===Inoculation and incubation at LB broth===
 
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<html>
 
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<ol>
 
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<li>Positive feedback circuit:</li>
 
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Pc-A-C-B, Pc-A-C-E, Pc-A-C-B, Pc-D-F-B, Pc-D-F-E, Pc-D-F-G;
 
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<li>Control:</li>
 
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PLas-B, PLas-E, PLas-G, PRhl-B, PRhl-E, PRhl-G;
 
-
(A: RBS-RhlR-tt C: PRhl-RBS-RhlR D: RBS-LasR-tt F: PLas-RBS-LasR B: RBS-mCherry-tt E: RBS-GFPmut-tt G: RBS-mRFP-tt )
 
-
</ol>
 
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</html>
 
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==7/3==
 
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===Inoculation and incubation at LB broth===
 
-
Pcin(resistant=A), CinR (resistant=K)
 
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===Check ===
 
-
 
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#Positive feedback circuit: 
 
-
Pc-A-C-B, Pc-A-C-E, Pc-A-C-B, Pc-D-F-B, Pc-D-F-E, Pc-D-F-G;
 
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#Control: 
 
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PLas-B, PLas-E, PLas-G, PRhl-B, PRhl-E, PRhl-G;
 
-
(A: RBS-RhlR-tt C: PRhl-RBS-RhlR D: RBS-LasR-tt F: PLas-RBS-LasR B: RBS-mCherry-tt E: RBS-GFPmut-tt G: RBS-mRFP-tt )
 
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==7/4==
 
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==7/5==
 
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= 7/8~7/12 =
 
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==7/8==
 
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==7/9==
 
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==7/10==
 
-
 
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==7/11==
 
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==7/12==
 
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=7/15~7/19=
 
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==7/15==
 
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==7/16==
 
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==7/17==
 
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==7/18==
 
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==7/19==
 
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=7/22~7/31=
 
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==7/22==
 
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==7/23==
 
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==7/24==
 
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==7/25==
 
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==7/26==
 
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==7/27==
 
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==7/28==
 
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==7/29==
 
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==7/30==
 
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==7/31==
 
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== July ==
 
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=2=
 
{{:Team:NTU-Taida/Templates/ContentEnd}}{{:Team:NTU-Taida/Templates/Footer|ActiveNavbar=Notebook}}
{{:Team:NTU-Taida/Templates/ContentEnd}}{{:Team:NTU-Taida/Templates/Footer|ActiveNavbar=Notebook}}

Latest revision as of 02:45, 28 September 2013

Retrieved from "http://2013.igem.org/Team:NTU-Taida/Notebook/Journal"