Team:NTU-Taida/Notebook/Journal

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==7/2==
==7/2==
 +
===Result:===
 +
There’s no colonies at plates of Pcin and CinR. 
 +
===Transform:===
 +
Pcin(resistant=A), CinR (resistant=K)
 +
===Inoculation and incubation at LB broth===
 +
<html>
 +
<ol>
 +
<li>Positive feedback circuit:</li>
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Pc-A-C-B, Pc-A-C-E, Pc-A-C-B, Pc-D-F-B, Pc-D-F-E, Pc-D-F-G;
 +
<li>Control:</li>
 +
PLas-B, PLas-E, PLas-G, PRhl-B, PRhl-E, PRhl-G;
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(A: RBS-RhlR-tt C: PRhl-RBS-RhlR D: RBS-LasR-tt F: PLas-RBS-LasR B: RBS-mCherry-tt E: RBS-GFPmut-tt G: RBS-mRFP-tt )
 +
</ol>
==7/3==
==7/3==

Revision as of 16:47, 2 September 2013

Journal

July
Sun. Mon. Tue. Wed. Thu. Fri. Sat.
01 02 03 04 05 06
07 08 09 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30 31

Contents

July

7/1~7/5

Finish RhlR and LasR circuit.

7/1

Transform:

  1. Positive feedback circuit:
    Pc-A-C-B, Pc-A-C-E, Pc-A-C-B, Pc-D-F-B, Pc-D-F-E, Pc-D-F-G;
  2. Control:
    PLas-B, PLas-E, PLas-G, PRhl-B, PRhl-E, PRhl-G;
    3. (A: RBS-RhlR-tt C: PRhl-RBS-RhlR D: RBS-LasR-tt F: PLas-RBS-LasR B: RBS-mCherry-tt E: RBS-GFPmut-tt G: RBS-mRFP-tt )
  3. New:
    Pcin, CinR (resistant=C)

7/2

Result:

There’s no colonies at plates of Pcin and CinR.

Transform:

Pcin(resistant=A), CinR (resistant=K)

Inoculation and incubation at LB broth

  1. Positive feedback circuit:
    2. Pc-A-C-B, Pc-A-C-E, Pc-A-C-B, Pc-D-F-B, Pc-D-F-E, Pc-D-F-G;
  2. Control:
    3. PLas-B, PLas-E, PLas-G, PRhl-B, PRhl-E, PRhl-G; (A: RBS-RhlR-tt C: PRhl-RBS-RhlR D: RBS-LasR-tt F: PLas-RBS-LasR B: RBS-mCherry-tt E: RBS-GFPmut-tt G: RBS-mRFP-tt )
==7/3== ==7/4== ==7/5== = 7/8~7/12 = ==7/8== ==7/9== ==7/10== ==7/11== ==7/12== =7/15~7/19= ==7/15== ==7/16== ==7/17== ==7/18== ==7/19== =7/22~7/31= ==7/22== ==7/23== ==7/24== ==7/25== ==7/26== ==7/27== ==7/28== ==7/29== ==7/30== ==7/31== == July == =2= {{:Team:NTU-Taida/Templates/ContentEnd}}{{:Team:NTU-Taida/Templates/Footer|ActiveNavbar=Notebook}}

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