"
Team:NTU-Taida/Notebook/Journal/June
From 2013.igem.org
(Difference between revisions)
(Created page with "{{:Team:NTU-Taida/Templates/Header}}{{:Team:NTU-Taida/Templates/Navbar}}{{:Team:NTU-Taida/Templates/Sidebar-calendar|Title=Journal|Month=July}}{{:Team:NTU-Taida/Templates/Content...") |
|||
| (6 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| - | {{:Team:NTU-Taida/Templates/Header}}{{:Team:NTU-Taida/Templates/Navbar}}{{:Team:NTU-Taida/Templates/Sidebar-calendar|Title=Journal|Month= | + | {{:Team:NTU-Taida/Templates/Header}}{{:Team:NTU-Taida/Templates/Navbar}}{{:Team:NTU-Taida/Templates/Sidebar-calendar|Title=Journal|Month=June}}{{:Team:NTU-Taida/Templates/ContentStart}} |
| - | + | <html><a name="603"></a></html> | |
=6/3= | =6/3= | ||
| - | |||
===Digest:=== | ===Digest:=== | ||
B0015 | B0015 | ||
| Line 38: | Line 37: | ||
RBS-mCherry-tt | RBS-mCherry-tt | ||
| - | + | <html><a name="604"></a></html> | |
=6/4= | =6/4= | ||
===Transform:=== | ===Transform:=== | ||
| Line 54: | Line 53: | ||
RBS-mCherry-tt | RBS-mCherry-tt | ||
| - | + | <html><a name="605"></a></html> | |
=6/5= | =6/5= | ||
===Inoculation & Incubation=== | ===Inoculation & Incubation=== | ||
| Line 70: | Line 69: | ||
RBS-mCherry-tt | RBS-mCherry-tt | ||
| - | + | <html><a name="607"></a></html> | |
=6/7= | =6/7= | ||
===Check:=== | ===Check:=== | ||
Latest revision as of 19:09, 27 September 2013
Contents |
6/3
Digest:
B0015
pRhl
pLas
RBS-mCherry
RBS-RhlR1
RBS-RhlR2
RBS-LasR1
RBS-LasR2
RBS-GFPmut
RBS-mRFP
Ligation overnight:
RBS-RhlR1-tt
RBS-LasR1-tt
pRhl-RBS-RhlR2
pLas RBS-LasR2
RBS-GFP-tt
RBS-mRFP-tt
6/4
Transform:
RBS-RhlR1-tt
RBS-LasR1-tt
pRhl-RBS-RhlR2
pLas RBS-LasR2
RBS-GFP-tt
RBS-mRFP-tt
6/5
Inoculation & Incubation
RBS-RhlR1-tt
RBS-LasR1-tt
pRhl-RBS-RhlR2
pLas RBS-LasR2
RBS-GFP-tt
RBS-mRFP-tt
6/7
Check:
RBS-RhlR1-tt
RBS-LasR1-tt
pRhl-RBS-RhlR2
pLas RBS-LasR2
RBS-GFP-tt
RBS-mRFP-tt
RBS-mCherry-tt
6/7
Get Plasmid miniprep:
A3 : 195.1 ng / μL
A4 : 218.9 ng / μL
B4 : 196.1 ng / μL
B5 : 226.2 ng / μL
C1 : 152.0 ng / μL
C4 : 148.3 ng / μL
D3 :198.3 ng / μL
D5 : 209.2 ng / μL
E1 : 226.1 ng / μL
E5 : 160.4 ng / μL
F1 : 185.4 ng / μL
F2 : 191.7 ng / μL
G3: 197.8 ng / μL
G4: 216.0 ng / μL
