Team:NTU-Taida/Parts

From 2013.igem.org

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{{:Team:NTU-Taida/Templates/Header}}{{:Team:NTU-Taida/Templates/Navbar}}{{:Team:NTU-Taida/Templates/Sidebar|Title=Part}}{{:Team:NTU-Taida/Templates/ContentStart}}
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=Original Biobrick=
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<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
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<table>
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<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
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<tr class="tableizer-firstrow"><th>Name</th><th>Type</th><th>Description</th><th>Length</th></tr>
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This is a template page. READ THESE INSTRUCTIONS.
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<tr><td>BBa_K1157000</td><td>Regulatory</td><td>promoter sequence for PQS quorum sensing</td><td>341</td></tr>
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</div>
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<tr><td>BBa_K1157001</td><td>Coding</td><td>PQSR</td><td>999</td></tr>
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<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
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<tr><td>BBa_K1157003</td><td>Coding</td><td>AbaR</td><td>597</td></tr>
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You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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</table>
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</div>
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</html>
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<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
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=Reporter=
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You <strong>MUST</strong>  have all of the pages listed in the menu below with the names specified.  PLEASE keep all of your pages within your teams namespace.  
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<table>
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<tr class="tableizer-firstrow"><th>Name</th><th>Type</th><th>Description</th><th>Length</th></tr>
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</div>
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<tr><td>BBa_K1157005</td><td>Reporter</td><td>Luciferase generator</td><td>1094</td></tr>
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<tr><td>&nbsp;</td><td>Reporter</td><td>RBS-mTagBFP-tt</td><td>849</td></tr>
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<tr><td>&nbsp;</td><td>&nbsp;</td><td>&nbsp;</td><td>&nbsp;</td></tr>
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<tr><td>&nbsp;</td><td>Reporter</td><td>RBS-mCherry-tt</td><td>858</td></tr>
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<tr><td>&nbsp;</td><td>&nbsp;</td><td>&nbsp;</td><td>&nbsp;</td></tr>
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<tr><td>&nbsp;</td><td>Reporter</td><td>RBS-GFP-tt</td><td>867</td></tr>
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<tr><td>&nbsp;</td><td>&nbsp;</td><td>&nbsp;</td><td></td></tr>
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</table>
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</html>
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=Protein Generator=
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<table>
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<tr class="tableizer-firstrow"><th>Name</th><th>Type</th><th>Description</th><th>Length</th></tr>
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<tr><td>BBa_K1157007</td><td>Coding</td><td>Quorum sensing receptor pqsR generator</td><td>1157</td></tr>
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<tr><td>BBa_K1157017</td><td>Composite</td><td>pPQS-GFPmut3</td><td>1227</td></tr>
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<tr><td>BBa_K1157019</td><td>Composite</td><td>Pc-PQSR</td><td>1200</td></tr>
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<tr><td>&nbsp;</td><td>&nbsp;</td><td>RBS-RhlR-tt</td><td>873</td></tr>
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<tr><td>&nbsp;</td><td>&nbsp;</td><td>&nbsp;</td><td>&nbsp;</td></tr>
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  <tr><td>&nbsp;</td><td>&nbsp;</td><td>RBS-LasR-tt</td><td>867</td></tr>
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  <tr><td>&nbsp;</td><td>&nbsp;</td><td>RBS-CinR-tt</td><td>931</td></tr>
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<tr><td>&nbsp;</td><td>&nbsp;</td><td>RBS-AbaR-tt</td><td>741</td></tr>
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</table>
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</html>
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=Without positive feedback circuits=
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<html>
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<table>
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<tr class="tableizer-firstrow"><th>Name</th><th>Type</th><th>Description</th><th>Length</th></tr>
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<tr><td>BBa_K1157010</td><td>Composite</td><td>Pc-RhlR-mCherry</td><td>1871</td></tr>
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<tr><td>BBa_K1157016</td><td>Composite</td><td>Pc-LasR-pLas-BFP</td><td>1960</td></tr>
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<tr><td>BBa_K575033</td><td>Composite</td><td>PRhi-GFP-Pc-RhlR</td><td>1600</td></tr>
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<tr><td>BBa K575024</td><td>Composite</td><td>Plas-GFP-Pc-LasR</td><td>1701</td></tr>
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<tr><td>BBa_K1157008</td><td>Composite</td><td>pLas-BFP-Pc-LasR</td><td>2018</td></tr>
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<tr><td>&nbsp;</td><td>&nbsp;</td><td>pPQS-GFP-Pc-PQSR</td><td>2401</td></tr>
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</table>
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</html>
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=Positive Feedback circuit=
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<html>
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<table>
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<tr class="tableizer-firstrow"><th>Name</th><th>Type</th><th>Description</th><th>Length</th></tr>
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<tr><td>BBa_K1157020</td><td>Composite</td><td>Pc-RhlR-pRhl-RhlR-GFPmut3</td><td>2635</td></tr>
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  <tr><td>BBa_K1157011</td><td>Composite</td><td>Pc-RhlR-pRhl-RhlR-BFP</td><td>2620</td></tr>
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  <tr><td>BBa_K1157012</td><td>Composite</td><td>Pc-RhlR-pRhl-RhlR-luciferase</td><td>2851</td></tr>
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<tr><td>BBa_K1157018</td><td>Composite</td><td>pRhl-RhlR-mCherry-Pc-RhlR</td><td>2629</td></tr>
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<tr><td>BBa_K1157021</td><td>Composite</td><td>pRhl-RhlR-GFP-Pc-RhlR</td><td>2635</td></tr>
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<tr><td>BBa_K1157014</td><td>Composite</td><td>pLas-LasR-GFPmut3-Pc-LasR</td><td>2727</td></tr>
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</table>
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</html>
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=Negative Regulation=
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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<table>
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!align="center"|[[Team:NTU-Taida|Home]]
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<tr class="tableizer-firstrow"><th>Name</th><th>Type</th><th>Description</th><th>Length</th></tr>
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!align="center"|[[Team:NTU-Taida/Team|Team]]
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<tr><td>BBa_K1157013</td><td>Composite</td><td>pCI-mCherry-pConst-RhlR-pRhl-RhlR-CI</td><td>3627</td></tr>
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=NTU-Taida Official Team Profile]
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  <tr><td>BBa_K1157015</td><td>Composite</td><td>pConst-Rhl-pRhl-RhlR-CI-pCI-mCherry</td><td>3627</td></tr>
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!align="center"|[[Team:NTU-Taida/Project|Project]]
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</table>
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!align="center"|[[Team:NTU-Taida/Parts|Parts Submitted to the Registry]]
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</html>
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!align="center"|[[Team:NTU-Taida/Modeling|Modeling]]
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!align="center"|[[Team:NTU-Taida/Notebook|Notebook]]
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!align="center"|[[Team:NTU-Taida/Safety|Safety]]
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!align="center"|[[Team:NTU-Taida/Attributions|Attributions]]
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|}
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An important aspect of the iGEM competition is the use and creation of standard biological parts. Each team will make new parts during iGEM and will place them in the [http://partsregistry.org Registry of Standard Biological Parts]. The iGEM software provides an easy way to present the parts your team has created . The "groupparts" tag will generate a table with all of the parts that your team adds to your team sandbox.  Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.
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Remember that the goal of proper part documentation is to describe and define a part such that it can be used without a need to refer to the primary literature. The next iGEM team should be able to read your documentation and be able to use the part successfully. Also, you should provide proper references to acknowledge previous authors and to provide for  users who wish to know more.
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<groupparts>iGEM013 NTU-Taida</groupparts>
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{{:Team:NTU-Taida/Templates/ContentEnd}}{{:Team:NTU-Taida/Templates/Footer|ActiveNavbar=Part}}

Revision as of 19:53, 27 September 2013

Part



Contents

Original Biobrick

NameTypeDescriptionLength
BBa_K1157000Regulatorypromoter sequence for PQS quorum sensing341
BBa_K1157001CodingPQSR999
BBa_K1157003CodingAbaR597

Reporter

NameTypeDescriptionLength
BBa_K1157005ReporterLuciferase generator1094
 ReporterRBS-mTagBFP-tt849
    
 ReporterRBS-mCherry-tt858
    
 ReporterRBS-GFP-tt867
   

</html>

Protein Generator

NameTypeDescriptionLength
BBa_K1157007CodingQuorum sensing receptor pqsR generator1157
BBa_K1157017CompositepPQS-GFPmut31227
BBa_K1157019CompositePc-PQSR1200
  RBS-RhlR-tt873
    
  RBS-LasR-tt867
  RBS-CinR-tt931
  RBS-AbaR-tt741

Without positive feedback circuits

NameTypeDescriptionLength
BBa_K1157010CompositePc-RhlR-mCherry1871
BBa_K1157016CompositePc-LasR-pLas-BFP1960
BBa_K575033CompositePRhi-GFP-Pc-RhlR1600
BBa K575024CompositePlas-GFP-Pc-LasR1701
BBa_K1157008CompositepLas-BFP-Pc-LasR2018
  pPQS-GFP-Pc-PQSR2401

Positive Feedback circuit

NameTypeDescriptionLength
BBa_K1157020CompositePc-RhlR-pRhl-RhlR-GFPmut32635
BBa_K1157011CompositePc-RhlR-pRhl-RhlR-BFP2620
BBa_K1157012CompositePc-RhlR-pRhl-RhlR-luciferase2851
BBa_K1157018CompositepRhl-RhlR-mCherry-Pc-RhlR2629
BBa_K1157021CompositepRhl-RhlR-GFP-Pc-RhlR2635
BBa_K1157014CompositepLas-LasR-GFPmut3-Pc-LasR2727

Negative Regulation

NameTypeDescriptionLength
BBa_K1157013CompositepCI-mCherry-pConst-RhlR-pRhl-RhlR-CI3627
BBa_K1157015CompositepConst-Rhl-pRhl-RhlR-CI-pCI-mCherry3627

Retrieved from "http://2013.igem.org/Team:NTU-Taida/Parts"