Team:NTU-Taida/Parts

From 2013.igem.org

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==Original Biobrick==
==Original Biobrick==
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<tr class="tableizer-firstrow"><th>Name</th><th>Type</th><th>Description</th><th>Length</th></tr>
<tr class="tableizer-firstrow"><th>Name</th><th>Type</th><th>Description</th><th>Length</th></tr>
  <tr><td><a href="http://parts.igem.org/Part:BBa_K1157000"> BBa_K1157000</a></td><td>Regulatory</td><td>promoter sequence for PQS quorum sensing</td><td>341</td></tr>
  <tr><td><a href="http://parts.igem.org/Part:BBa_K1157000"> BBa_K1157000</a></td><td>Regulatory</td><td>promoter sequence for PQS quorum sensing</td><td>341</td></tr>
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==Reporter==
==Reporter==
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  <tr><td><a href="http://parts.igem.org/Part:BBa_K1157005">BBa_K1157005</a></td><td>Reporter</td><td>Luciferase generator</td><td>1094</td></tr>
  <tr><td><a href="http://parts.igem.org/Part:BBa_K1157005">BBa_K1157005</a></td><td>Reporter</td><td>Luciferase generator</td><td>1094</td></tr>
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==Protein Generator==
==Protein Generator==
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  <tr><td><a href="http://parts.igem.org/Part:BBa_K1157007">BBa_K1157007</a></td><td>Coding</td><td>Quorum sensing receptor pqsR generator</td><td>1157</td></tr>
  <tr><td><a href="http://parts.igem.org/Part:BBa_K1157007">BBa_K1157007</a></td><td>Coding</td><td>Quorum sensing receptor pqsR generator</td><td>1157</td></tr>
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==Without positive feedback circuits==
==Without positive feedback circuits==
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  <tr><td><a href="http://parts.igem.org/Part:BBa_K1157010">BBa_K1157010</a></td><td>Composite</td><td>Pc-RhlR-mCherry</td><td>1871</td></tr>
  <tr><td><a href="http://parts.igem.org/Part:BBa_K1157010">BBa_K1157010</a></td><td>Composite</td><td>Pc-RhlR-mCherry</td><td>1871</td></tr>
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==Positive Feedback circuit==
==Positive Feedback circuit==
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  <tr><td><a href="http://parts.igem.org/Part:BBa_K1157020">BBa_K1157020</a></td><td>Composite</td><td>Pc-RhlR-pRhl-RhlR-GFPmut3</td><td>2635</td></tr>
  <tr><td><a href="http://parts.igem.org/Part:BBa_K1157020">BBa_K1157020</a></td><td>Composite</td><td>Pc-RhlR-pRhl-RhlR-GFPmut3</td><td>2635</td></tr>
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==Negative Regulation==
==Negative Regulation==
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  <tr><td><a href="http://parts.igem.org/Part:BBa_K1157013">BBa_K1157013</a></td><td>Composite</td><td>pCI-mCherry-pConst-RhlR-pRhl-RhlR-CI</td><td>3627</td></tr>
  <tr><td><a href="http://parts.igem.org/Part:BBa_K1157013">BBa_K1157013</a></td><td>Composite</td><td>pCI-mCherry-pConst-RhlR-pRhl-RhlR-CI</td><td>3627</td></tr>

Revision as of 21:19, 27 September 2013

Part



Contents

Note

Some plasmids are not submitted due to similar parts in registry or time limit.

Also, BBa_K575024 and BBa_K575033 were submitted from the Northwestern.

However,they are also important fragments in our projects and we still lists them below.

Original Biobrick

NameTypeDescriptionLength
BBa_K1157000Regulatorypromoter sequence for PQS quorum sensing341
BBa_K1157001CodingPQSR999
BBa_K1157003CodingAbaR597

Reporter

NameTypeDescriptionLength
BBa_K1157005ReporterLuciferase generator1094
 ReporterRBS-mTagBFP-tt849
    
 ReporterRBS-mCherry-tt858
    
 ReporterRBS-GFP-tt867
   

Protein Generator

NameTypeDescriptionLength
BBa_K1157007CodingQuorum sensing receptor pqsR generator1157
BBa_K1157017CompositepPQS-GFPmut31227
BBa_K1157019CompositePc-PQSR1200
 CompositeRBS-RhlR-tt873
 Composite  
 CompositeRBS-LasR-tt867
 CompositeRBS-CinR-tt931
 CompositeRBS-AbaR-tt741

Without positive feedback circuits

NameTypeDescriptionLength
BBa_K1157010CompositePc-RhlR-mCherry1871
BBa_K1157016CompositePc-LasR-pLas-BFP1960
BBa_K575033CompositePRhl-GFP-Pc-RhlR1600
BBa_K575024CompositePlas-GFP-Pc-LasR1701
BBa_K1157008CompositepLas-BFP-Pc-LasR2018
 CompositepPQS-GFP-Pc-PQSR2401

Positive Feedback circuit

NameTypeDescriptionLength
BBa_K1157020CompositePc-RhlR-pRhl-RhlR-GFPmut32635
BBa_K1157011CompositePc-RhlR-pRhl-RhlR-BFP2620
BBa_K1157012CompositePc-RhlR-pRhl-RhlR-luciferase2851
BBa_K1157018CompositepRhl-RhlR-mCherry-Pc-RhlR2629
BBa_K1157021CompositepRhl-RhlR-GFP-Pc-RhlR2635
BBa_K1157014CompositepLas-LasR-GFPmut3-Pc-LasR2727

Negative Regulation

NameTypeDescriptionLength
BBa_K1157013CompositepCI-mCherry-pConst-RhlR-pRhl-RhlR-CI3627
BBa_K1157015CompositepConst-Rhl-pRhl-RhlR-CI-pCI-mCherry3627