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Team:Calgary/Notebook/Protocols
From 2013.igem.org
(Difference between revisions)
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<li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/ColonyPCR" target="_blank">Colony PCR</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/ColonyPCR" target="_blank">Colony PCR</a></li> | ||
<li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/GelExtraction" target="_blank">Gel Extraction</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/GelExtraction" target="_blank">Gel Extraction</a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/GibsonAssembly" target="_blank">Gibson Assembly</a></li> | ||
<li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/KapaPCR" target="_blank">Kapa PCR</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/KapaPCR" target="_blank">Kapa PCR</a></li> | ||
<li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/PCRPurification" target="_blank">PCR Purification</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/PCRPurification" target="_blank">PCR Purification</a></li> | ||
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<li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/FreezeThawProtocolforProteinSamples" target="_blank">Freeze-Thaw Protocol for Protein Samples</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/FreezeThawProtocolforProteinSamples" target="_blank">Freeze-Thaw Protocol for Protein Samples</a></li> | ||
<li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/GlassBeadsCellLysisProtocolforProteinSamples" target="_blank">Glass Beads Cell Lysis Protocol for Protein Samples</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/GlassBeadsCellLysisProtocolforProteinSamples" target="_blank">Glass Beads Cell Lysis Protocol for Protein Samples</a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/MiniExpression" target="_blank">Mini Expression</a></li> | ||
<li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/OsmoticShock" target="_blank">Osmotic Shock</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/OsmoticShock" target="_blank">Osmotic Shock</a></li> | ||
<li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/SDSPAGEGel" target="_blank">SDS PAGE Gel</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/SDSPAGEGel" target="_blank">SDS PAGE Gel</a></li> | ||
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<li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/PrussianBlueFerritinPHOptimization" target="_blank">Prussian Blue Ferritin pH Optimization</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/PrussianBlueFerritinPHOptimization" target="_blank">Prussian Blue Ferritin pH Optimization</a></li> | ||
<li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/PrussianBlueFerritinTemperatureOptimization" target="_blank">Prussian Blue Ferritin Temperature Optimization</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/PrussianBlueFerritinTemperatureOptimization" target="_blank">Prussian Blue Ferritin Temperature Optimization</a></li> | ||
| + | </ul> | ||
| + | <h2>Beta-Lactamase Protocols</h2> | ||
| + | <ul> | ||
| + | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/AmpicillinSurvivalAssay" target="_blank">Ampicillin Survival Assay</a></li> | ||
</ul> | </ul> | ||
</section> | </section> | ||
</html> | </html> | ||
Revision as of 02:19, 26 September 2013
Protocols
General Protocols
- Bacterial Transformation
- Glycerol Stocks
- LB Agar Plates
- Making Chemically Competent E. coli Cells
- Overnight Cultures
DNA Protocols
- Agarose Gel Electrophoresis
- Colony PCR
- Gel Extraction
- Gibson Assembly
- Kapa PCR
- PCR Purification
- Plasmid Purification from E. coli
- Rehydration of Registry DNA
- Restriction Digestion, Antarctic Phosphatase, and Ligation
- Verification Digest
Protein Protocols
- Bradford Assay
- Freeze-Thaw Protocol for Protein Samples
- Glass Beads Cell Lysis Protocol for Protein Samples
- Mini Expression
- Osmotic Shock
- SDS PAGE Gel
- Western Blot
TALE Protocols
Ferritin Protocols
- Kinetics
- Prussian Blue Ferritin Michaelis-Menten Kinetic Analysis
- Prussian Blue Ferritin pH Optimization
- Prussian Blue Ferritin Temperature Optimization
