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Team:Calgary/Notebook/Protocols
From 2013.igem.org
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<li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/GlassBeadsCellLysisProtocolforProteinSamples" target="_blank">Glass Beads Cell Lysis Protocol for Protein Samples</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/GlassBeadsCellLysisProtocolforProteinSamples" target="_blank">Glass Beads Cell Lysis Protocol for Protein Samples</a></li> | ||
<li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/MiniExpression" target="_blank">Mini Expression</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/MiniExpression" target="_blank">Mini Expression</a></li> | ||
| + | <li>href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/MiniExpression" target="_blank">Large Scale Protein expression and Purification</a></li> | ||
<li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/NativePAGEGel" target="_blank">Native PAGE Gel</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/NativePAGEGel" target="_blank">Native PAGE Gel</a></li> | ||
<li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/OsmoticShock" target="_blank">Osmotic Shock</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/OsmoticShock" target="_blank">Osmotic Shock</a></li> | ||
Revision as of 09:37, 27 September 2013
Protocols
General Protocols
- Bacterial Transformation
- Glycerol Stocks
- LB Agar Plates
- Making Chemically Competent E. coli Cells
- Overnight Cultures
DNA Protocols
- Agarose Gel Electrophoresis
- Colony PCR
- Gel Extraction
- Gibson Assembly
- Kapa PCR
- PCR Purification
- Plasmid Purification from E. coli
- Rehydration of Registry DNA
- Restriction Digestion, Antarctic Phosphatase, and Ligation
- Verification Digest
Protein Protocols
- Bradford Assay
- Freeze-Thaw Cell Lysis Protocol for Protein Samples
- Glass Beads Cell Lysis Protocol for Protein Samples
- Mini Expression
- href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/MiniExpression" target="_blank">Large Scale Protein expression and Purification
- Native PAGE Gel
- Osmotic Shock
- SDS PAGE Gel
- Western Blot
TALE Protocols
Ferritin Protocols
- Prussian Blue Ferritin Michaelis-Menten Kinetic Analysis
- Prussian Blue Ferritin pH Optimization
- Prussian Blue Ferritin Synthesis
- Prussian Blue Ferritin Temperature Optimization
