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Team:Calgary/Notebook/Protocols
From 2013.igem.org
(Difference between revisions)
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<li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/PlasmidPurificationfromEcoli" target="_blank">Plasmid Purification from E. coli</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/PlasmidPurificationfromEcoli" target="_blank">Plasmid Purification from E. coli</a></li> | ||
<li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/RehydrationOfRegistryDNA" target="_blank">Rehydration of Registry DNA</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/RehydrationOfRegistryDNA" target="_blank">Rehydration of Registry DNA</a></li> | ||
| - | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/RestrictionDigestionAntarcticPhosphataseAndLigation" target="_blank">Restriction Digestion, Antarctic Phosphatase, | + | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/RestrictionDigestionAntarcticPhosphataseAndLigation" target="_blank">Restriction Digestion, Antarctic Phosphatase, & Ligation</a></li> |
<li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/VerificationDigest" target="_blank">Verification Digest</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/VerificationDigest" target="_blank">Verification Digest</a></li> | ||
</ul> | </ul> | ||
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<li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/FreezeThawProtocolforProteinSamples" target="_blank">Freeze-Thaw Protocol for Protein Samples</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/FreezeThawProtocolforProteinSamples" target="_blank">Freeze-Thaw Protocol for Protein Samples</a></li> | ||
<li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/GlassBeadsCellLysisProtocolforProteinSamples" target="_blank">Glass Beads Cell Lysis Protocol for Protein Samples</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/GlassBeadsCellLysisProtocolforProteinSamples" target="_blank">Glass Beads Cell Lysis Protocol for Protein Samples</a></li> | ||
| - | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/LargeScaleProteinExpressionAndPurification" target="_blank">Large Scale Protein Expression | + | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/LargeScaleProteinExpressionAndPurification" target="_blank">Large Scale Protein Expression & Purification</a></li> |
<li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/MiniExpression" target="_blank">Mini Expression</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/MiniExpression" target="_blank">Mini Expression</a></li> | ||
<li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/NativePAGEGel" target="_blank">Native PAGE Gel</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/NativePAGEGel" target="_blank">Native PAGE Gel</a></li> | ||
Revision as of 09:48, 27 September 2013
Protocols
General Protocols
- Bacterial Transformation
- Glycerol Stocks
- LB Agar Plates
- Making Chemically Competent E. coli Cells
- Overnight Cultures
DNA Protocols
- Agarose Gel Electrophoresis
- Colony PCR
- Gel Extraction
- Gibson Assembly
- Kapa PCR
- PCR Purification
- Plasmid Purification from E. coli
- Rehydration of Registry DNA
- Restriction Digestion, Antarctic Phosphatase, & Ligation
- Verification Digest
Protein Protocols
- Bradford Assay
- Freeze-Thaw Protocol for Protein Samples
- Glass Beads Cell Lysis Protocol for Protein Samples
- Large Scale Protein Expression & Purification
- Mini Expression
- Native PAGE Gel
- Osmotic Shock
- SDS PAGE Gel
- Western Blot
TALE Protocols
Ferritin Protocols
- Prussian Blue Ferritin Michaelis-Menten Kinetic Analysis
- Prussian Blue Ferritin pH Optimization
- Prussian Blue Ferritin Synthesis
- Prussian Blue Ferritin Temperature Optimization
