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Team:Calgary/Notebook/Protocols
From 2013.igem.org
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<li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/KapaPCR" target="_blank">Kapa PCR</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/KapaPCR" target="_blank">Kapa PCR</a></li> | ||
<li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/PCRPurification" target="_blank">PCR Purification</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/PCRPurification" target="_blank">PCR Purification</a></li> | ||
| - | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/PlasmidPurificationfromEcoli" target="_blank">Plasmid Purification from | + | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/PlasmidPurificationfromEcoli" target="_blank">Plasmid Purification from E. coli</a></li> |
<li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/RehydrationOfRegistryDNA" target="_blank">Rehydration of Registry DNA</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/RehydrationOfRegistryDNA" target="_blank">Rehydration of Registry DNA</a></li> | ||
<li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/RestrictionDigestionAntarcticPhosphataseAndLigation" target="_blank">Restriction Digestion, Antarctic Phosphatase, & Ligation</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/RestrictionDigestionAntarcticPhosphataseAndLigation" target="_blank">Restriction Digestion, Antarctic Phosphatase, & Ligation</a></li> | ||
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<li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/FreezeThawProtocolforProteinSamples" target="_blank">Freeze-Thaw Protocol for Protein Samples</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/FreezeThawProtocolforProteinSamples" target="_blank">Freeze-Thaw Protocol for Protein Samples</a></li> | ||
<li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/GlassBeadsCellLysisProtocolforProteinSamples" target="_blank">Glass Beads Cell Lysis Protocol for Protein Samples</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/GlassBeadsCellLysisProtocolforProteinSamples" target="_blank">Glass Beads Cell Lysis Protocol for Protein Samples</a></li> | ||
| + | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/Immunoprecipitation" target="_blank">Immunoprecipitation</a></li> | ||
<li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/LargeScaleProteinExpressionAndPurification" target="_blank">Large Scale Protein Expression & Purification</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/LargeScaleProteinExpressionAndPurification" target="_blank">Large Scale Protein Expression & Purification</a></li> | ||
<li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/MiniExpression" target="_blank">Mini Expression</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/MiniExpression" target="_blank">Mini Expression</a></li> | ||
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<h2>TALE Protocols</h2> | <h2>TALE Protocols</h2> | ||
<ul> | <ul> | ||
| + | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/FunctionalityAssayOnNitrocellulose" target="_blank">Functionality Assay on Nitrocellulose</a></li> | ||
<li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/GelShiftAssay" target="_blank">Gel Shift Assay</a></li> | <li><a href="https://2013.igem.org/Team:Calgary/Notebook/Protocols/GelShiftAssay" target="_blank">Gel Shift Assay</a></li> | ||
</ul> | </ul> | ||
Revision as of 01:12, 28 October 2013
Protocols
General Protocols
- Bacterial Transformation
- Glycerol Stocks
- LB Agar Plates
- Making Chemically Competent E. coli Cells
- Overnight Cultures
DNA Protocols
- Agarose Gel Electrophoresis
- Colony PCR
- Gel Extraction
- Gibson Assembly
- Kapa PCR
- PCR Purification
- Plasmid Purification from E. coli
- Rehydration of Registry DNA
- Restriction Digestion, Antarctic Phosphatase, & Ligation
- Verification Digest
Protein Protocols
- Bradford Assay
- Freeze-Thaw Protocol for Protein Samples
- Glass Beads Cell Lysis Protocol for Protein Samples
- Immunoprecipitation
- Large Scale Protein Expression & Purification
- Mini Expression
- Native PAGE Gel
- Osmotic Shock
- SDS PAGE Gel
- Western Blot
TALE Protocols
Ferritin Protocols
- Nitrocellulose Assay
- Prussian Blue Ferritin Michaelis-Menten Kinetic Analysis
- Prussian Blue Ferritin pH Optimization
- Prussian Blue Ferritin Synthesis
- Prussian Blue Ferritin Temperature Optimization
