Protocols

General Protocols

• Bacterial Transformation
• Glycerol Stocks
• LB Agar Plates
• Making Chemically Competent E. coli Cells
• Overnight Cultures

DNA Protocols

• Agarose Gel Electrophoresis
• Colony PCR
• Gel Extraction
• Gibson Assembly
• Kapa PCR
• PCR Purification
• Plasmid Purification from E. coli
• Rehydration of Registry DNA
• Restriction Digestion, Antarctic Phosphatase, and Ligation
• Verification Digest

Protein Protocols

• Bradford Assay
• Freeze-Thaw Protocol for Protein Samples
• Glass Beads Cell Lysis Protocol for Protein Samples
• Mini Expression
• Osmotic Shock
• SDS PAGE Gel
• Western Blot

TALE Protocols

• Gel Shift Assay

Ferritin Protocols

• Prussian Blue Ferritin Michaelis-Menten Kinetic Analysis
• Prussian Blue Ferritin pH Optimization
• Prussian Blue Ferritin Temperature Optimization

Beta-Lactamase Protocols

• Ampicillin Survival Assay