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Team:SUSTC-Shenzhen-A/Notebook
From 2013.igem.org
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<a href="/index. check the function of the xylose and the nitrate inducible promote">Check he function of the xylose and the nitrate inducible promote</a><br> | <a href="/index. check the function of the xylose and the nitrate inducible promote">Check he function of the xylose and the nitrate inducible promote</a><br> | ||
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Revision as of 17:54, 27 September 2013
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Notebook
We started our project in middle July. You can find every detail by clicking the date.
18th July to 10th Aug: Training
12th Aug to 17th Sep: Project and biobricks.
Protocol
Preparation of LB culturePreparation of some antibiotic solution
Transformation
Preservation of the bacteria
Extract the target DNA
The use of spectrometer
Usage of PCR-Cycler
Usage of ultraviolet spectrophotometer
Preparation for the M9 culture
Preparation of the nitrate LB culture
Usage of FCM
Preparation of the gel
Recycle of the DNA in the gel and deal with gel
The recycle of the products of the PCR
Check he function of the xylose and the nitrate inducible promote
 The use of the microplate reader
