Team:SUSTC-Shenzhen-A/Notebook

From 2013.igem.org

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(Protocol)
(Protocol)
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="/index.Usage of PCR-Cycler">Usage of PCR-Cycler</a><br>
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="/index.Usage of PCR-Cycler">Usage of PCR-Cycler</a><br>
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="/index.Usage of ultraviolet spectrophotometer">Usage of ultraviolet spectrophotometer</a><br>
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="/index.Usage of ultraviolet spectrophotometer">Usage of ultraviolet spectrophotometer</a><br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="/index.Preparation for the M9 culture"> Preparation for the M9 culture</a><br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="/index. Preparation for the M9 culture"> Preparation for the M9 culture</a><br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="/index.Preparation of the nitrate LB culture">Preparation of the nitrate LB culture</a><br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="/index. Preparation of the nitrate LB culture">Preparation of the nitrate LB culture</a><br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="/index.Usage of FCM"> Usage of FCM</a><br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="/index. Usage of FCM"> Usage of FCM</a><br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="/index.Preparation of the gel">Preparation of the gel</a><br>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="/index. Preparation of the gel">Preparation of the gel</a><br>
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="/index.Recycle of the DNA in the gel and deal with gel"> Recycle of the DNA in the gel and deal with gel</a><br>
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="/index.Recycle of the DNA in the gel and deal with gel"> Recycle of the DNA in the gel and deal with gel</a><br>
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="/index.The recycle of the products of the PCR">The recycle of the products of the PCR</a><br>
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<a href="/index.The recycle of the products of the PCR">The recycle of the products of the PCR</a><br>

Revision as of 22:30, 27 September 2013

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Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Protocol and notes Safety and security Attributions Human Practice

July
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Notebook



    We started our project in middle July. You can find every detail by clicking the date.


    18th July to 10th Aug: Training


    12th Aug to 17th Sep: Project and biobricks.

Protocol

         Preparation of LB culture
         Preparation of some antibiotic solution
         Transformation
         Preservation of the bacteria
         Extract the target DNA
         The use of spectrometer
         Usage of PCR-Cycler
         Usage of ultraviolet spectrophotometer
          Preparation for the M9 culture
         Preparation of the nitrate LB culture
          Usage of FCM
         Preparation of the gel
          Recycle of the DNA in the gel and deal with gel
                            The recycle of the products of the PCR
                            The use of the microplate reader
                            Check he function of the xylose and the nitrate inducible promote