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Team:SUSTC-Shenzhen-A/Notebook
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!align="center"|[https://igem.org/Team.cgi?year=2013&team_name=SUSTC-Shenzhen-A Official Team Profile] | !align="center"|[https://igem.org/Team.cgi?year=2013&team_name=SUSTC-Shenzhen-A Official Team Profile] | ||
!align="center"|[[Team:SUSTC-Shenzhen-A/Project|Project]] | !align="center"|[[Team:SUSTC-Shenzhen-A/Project|Project]] | ||
| - | !align="center"|[[Team:SUSTC-Shenzhen-A/ | + | !align="center"|[[Team:SUSTC-Shenzhen-A/Part|Parts Submitted to the Registry]] |
!align="center"|[[Team:SUSTC-Shenzhen-A/Modeling|Modeling]] | !align="center"|[[Team:SUSTC-Shenzhen-A/Modeling|Modeling]] | ||
!align="center"|[[Team:SUSTC-Shenzhen-A/Notebook|Protocol and notes]] | !align="center"|[[Team:SUSTC-Shenzhen-A/Notebook|Protocol and notes]] | ||
Revision as of 03:18, 28 September 2013
| Home | Team | Official Team Profile | Project | Parts Submitted to the Registry | Modeling | Protocol and notes | Safety and security | Attributions | Human Practice |
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Notebook
We started our project in middle July. You can find every detail by clicking the date.
18th July to 10th Aug: Training
12th Aug to 17th Sep: Project and biobricks.
Protocol
Preparation of LB culturePreparation of some antibiotic solution
Transformation
Preservation of the bacteria
Extract the target DNA
The use of spectrometer
Usage of PCR-Cycler
Usage of ultraviolet spectrophotometer
Preparation for the M9 culture
Preparation of the nitrate LB culture
Usage of FCM
Preparation of the gel
Recycle of the DNA in the gel and deal with gel
The recycle of the products of the PCR
The use of the microplate reader
Check he function of the xylose and the nitrate inducible promote
