Search results

From 2013.igem.org

Create the page "Plasmid" on this wiki!

Page title matches

Team:DTU-Denmark/Methods/Plasmid isolation
{{:Team:DTU-Denmark/Templates/StartPage|Plasmid Isolation by ethanol precipitation}}

902 B (140 words) - 17:40, 15 August 2013
Team:Macquarie Australia/Protocols/Plasmid Prep
<h1>Plasmid Prep</h1>

1 KB (201 words) - 06:04, 13 September 2013
Team:Washington/ISOLATION OF PLASMID
<h1>Isolation of Plasmid DNA (miniprep)</h1>

3 KB (540 words) - 04:31, 27 September 2013
Team:Freiburg/Notebook/lab crrna plasmid
...ef="https://2013.igem.org/Team:Freiburg/Notebook/lab_crrna_plasmid"> crRNA-Plasmid </a> ...tps://2013.igem.org/Team:Freiburg/Notebook/lab_gfp_reporter"> GFP-reporter-plasmid </a> 

16 KB (2,227 words) - 05:12, 28 October 2013
Team:Tokyo Tech/Experiment/pSB-M13 Plasmid Assay
pSB-M13 Plasmid Assay ...Tech/Project/M13_supplement#1._M13_plasmid_construction See more about our plasmid construction]).

4 KB (644 words) - 17:14, 28 October 2013
Team:USTC CHINA/Notebook/Protocols/Plasmid mini-prep
...ttps://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Plasmid mini-prep">Plasmid mini-prep</a></div></div> <h1>Plasmid mini-prep</h1>

6 KB (834 words) - 16:50, 27 September 2013

Page text matches

Team:TU-Munich/Notebook/Labjournal
Sequencing batch were prepared after manufacturer's protocol. (15 µl of plasmid DNA (50 - 100 ng) and 2 µl sequencing primer) * pSB1C3 plasmid with BBa_K801031 (PhyB 2 - 908 aa, RFC25): Colonies were picked from chlora

804 KB (118,126 words) - 22:30, 22 October 2013
Team:XMU Software/html
...t"> <table id="i0s1i1" class="C4"> <tbody><tr> <th colspan="3">Plasmid Extraction</th> <td rowspan="9" class="C4_td_textarea"> <textare ...t"> <table id="i0s2i6" class="C4"> <tbody><tr> <th colspan="3">Plasmid Extraction</th> <td rowspan="9" class="C4_td_textarea"> <textare

411 KB (51,065 words) - 05:18, 27 October 2013
Team:Freiburg/Project/toolkit
...g our plasmids, you will have to clone your target sequence into our crRNA plasmid (protocol see below). <td> RNAimer - plasmid (crRNA - plasmid) </td>

87 KB (13,257 words) - 00:59, 29 October 2013
Team:SJTU-BioX-Shanghai/Notebook/Lab log
===1.Template Plasmid Amplification from Kit Plate=== ===2.Construct consititutional backbone plasmid===

13 KB (1,826 words) - 01:11, 29 October 2013
Team:Newcastle/Notebook/calendar
...SB1C3] then PCR the insert before integrating it into the amyE integration plasmid (pSBBs1C). We discovered that the Kanamycin resistance is an ''E *Groningen plasmid Mini-prep

113 KB (17,146 words) - 14:46, 30 September 2013
Team:Goettingen/NoteBook w13
...39">part6.4A C7 (102.6 ng/µl, 23.8.13, 3 µl plasmid + 1 µl HPLC water + 1 µl Primer 1:20)</td><td class="c13-21">< ...ot seen, but the band of re-ligated vectors was observed for re-ligand and plasmid control (> 1 kb, as expected) and also for B C5→ since this band is no

134 KB (22,952 words) - 21:14, 30 September 2013
Team:Paris Bettencourt/Notebook
<TR><TD>Template Plasmid</TD><TD>0.25 ul</TD></TR> for Backbone (M13mp18 plasmid): 3ug 

522 KB (75,300 words) - 03:51, 29 October 2013
Team:Goettingen/NoteBook w11
...omoter1rev , Promoter3rev or RBSrev, Mini Plasmid Prep of clones inoculated on 15.6.13 and preparation of some cryostocks, Te ... 6 – DarRrev C4 + VR (5 µl plasmid + 1 µl HPLC water + 1 µl iGEM_39 1:5)

105 KB (20,098 words) - 21:18, 30 September 2013
Team:Goettingen/NoteBook w12
...pan>          for all samples: 6 μl plasmid + 1 μl iGEM_38 1:5 or iGEM_39 1:5Seq number</td><td class="c12-28">Plasmid + primer</td></tr><tr><td class="c12-50">

165 KB (28,551 words) - 08:53, 17 October 2013
Team:HokkaidoU Japan/Shuffling Kit/Primer Designer
...369px 0}#plasmid-map #plasmid-9.plasmid-gray{background-position:-442px 0}#plasmid-map span{position:absolute;top:40px;left:-5px}.my-form{width:100%}.my-form .... Secondly, you hove to determine how many CDS are included in your target plasmid.</h3>

15 KB (1,973 words) - 02:50, 29 October 2013
Team:XMU-China/wetlab journal
Lane1:12D plasmid Lane2:12D plasmid, digestion of EcoRI,SpeI

93 KB (15,921 words) - 03:09, 28 September 2013
Team:Heidelberg/Project/Tag-Optimization
...fficiency. Since this combinatorial approach requires the cloning of large plasmid libraries and hundreds of cotransformations, we established HiCT: High-thro ... host strains were previously transformed with a pSB3K3 derived expression plasmid coding for the PPTase Sfp from B. subtilis, which is commonly used i

133 KB (19,473 words) - 02:56, 29 October 2013
Team:Freiburg/Notebook/standardisation
...only one band), new pSB1C3 is searched with RFC25 (–> Team Freiburg 2010 plasmid number 51).</ <h4>PCR of Fusion PCR1-7 and PCR over non-nickase Cas9 (plasmid 2004)</h4>

128 KB (13,537 words) - 05:10, 28 October 2013
Team:Freiburg/Notebook/lab assembly biobricks
...only one band), new pSB1C3 is searched with RFC25 (–> Team Freiburg 2010 plasmid number 51).</ <h5>PCR of Fusion PCR1-7 and PCR over non-nickase Cas9 (plasmid 2004)</h5>

132 KB (14,192 words) - 09:51, 1 October 2013
Team:SJTU-BioX-Shanghai/Notebook/Lab log/July
===Template Plasmid Amplification from Kit Plate=== ===Construct consititutional backbone plasmid===

5 KB (725 words) - 03:17, 28 September 2013
Team:SJTU-BioX-Shanghai/Notebook/Lab log/August
===Construct dCas9, PcyA and Ho1 plasmid.=== ====PcyA-pRSF plasmid construction====

5 KB (732 words) - 04:10, 28 September 2013
Team:OUC-China/Lab note
2. Agarose electrophoresis: detect the plasmid PCR product. ...;">Experiment: 1. Agarose electrophoresis: detect the plasmid PCR product. 

81 KB (11,222 words) - 03:53, 28 September 2013
Team:Evry/Notebook/Test
...rains are competent or not, we also transformed our bacteria with a pSB1A3 plasmid (red colonies) and plated them on LB medium with ampicillin only. <li> Plasmid 1K3

113 KB (15,206 words) - 01:49, 5 October 2013
Team:Evry/Notebook
...rains are competent or not, we also transformed our bacteria with a pSB1A3 plasmid (red colonies) and plated them on LB medium with ampicillin only. <li> Plasmid 1K3

117 KB (16,033 words) - 02:34, 29 October 2013
Team:OU-Norman OK/Project/Notebook
<div align="center" style="border:1px solid white">PCR of i-GEM Plasmid Backbones Continued</div> <div align="center" style="border:1px solid white">Adjusted PCR of iGEM Plasmid Backbones</div>

189 KB (32,035 words) - 04:17, 28 September 2013
Jsher
... to a gate that had 15 of the baseline (which was 38 or our cells with our plasmid) ...tures of 64, 65, 66 in LB Kan (Kan is 1000x) for MAGE. These all have our plasmid already

64 KB (10,685 words) - 04:42, 16 January 2014
J Sher
... to a gate that had 15 of the baseline (which was 38 or our cells with our plasmid) ...tures of 64, 65, 66 in LB Kan (Kan is 1000x) for MAGE. These all have our plasmid already

64 KB (10,685 words) - 04:40, 16 January 2014
Team:UChicago/Notebook
:*Plasmid Design *The plasmid design team decided on gibson assembly to produce the kerA biobrick with ge

68 KB (10,479 words) - 02:05, 28 September 2013
Team:Waterloo
... packaged itself due to a missing packaging sequence is called a “helper plasmid”. ... phagemid” and will be transmitted from sender cells that carry a helper plasmid

182 KB (26,875 words) - 03:50, 29 October 2013
Team:BYU Provo/Cholera - Detection
One possible question is how to get a plasmid to form with medicine or something inside. Today I looked over existing plasmid constructs. Apparently iGEMers from last year did clone many genes into pla

54 KB (8,926 words) - 20:11, 29 July 2013
Team:ITB Indonesia/Notebook/WetLab
Transform the plasmid to DH5α competent cell. We compared number of colony from CaCl2 method an <li>Preparing solutions for plasmid isolation by alkali lysis method</li>

25 KB (3,936 words) - 03:05, 28 September 2013
Cholera - Detection
One possible question is how to get a plasmid to form with medicine or something inside. Today I looked over existing plasmid constructs. Apparently iGEMers from last year did clone many genes into pla

48 KB (8,065 words) - 22:53, 22 May 2013
Team:Leicester/Notebook
<h3 class="nb">Transformation of RFP control plasmid</h3> Negative control (No plasmid) 

117 KB (15,598 words) - 00:32, 5 October 2013
Team:Heidelberg/Templates/DelH week22
===Generation of DelH Plasmid pHM04 18-09=== ===Generation of DelH Plasmid pHM04 20-09===

21 KB (3,010 words) - 21:36, 25 October 2013
Team:Frankfurt/Project/Methods
... transformation. Also only one restriction enzyme for linearization of the plasmid is needed. ...a new way of assembling BioBrick devices in a desired order to a targeting plasmid using homologue recombination system of yeast. It is called ''Yeast BioBric

23 KB (3,829 words) - 01:55, 5 October 2013
Team:Freiburg/Notebook/lab hormon
<li>1 µl of plasmid (~ 20 ng/µl) was added to 25 µl of chemically competent E. coli ce using Roti-Prep Plasmid Mini from Roth

64 KB (10,342 words) - 05:16, 28 October 2013
Team:Goettingen/NoteBook w6
Mini Plasmid Preparation, Test Digest of Clone 12 RBS-GFP-Terminator (see gel 11.07.) wi ...-bidi-font-weight:normal">Mini Plasmid Preparation<o:p></o:p>

387 KB (51,437 words) - 21:20, 30 September 2013
Team:Duke/Notebook/Lab Notebook
*Matt Farnitano built plasmid iGEM.1 with Golden Gate reaction from backbone, ACT1 promoter, YEVenus repo *CC built plasmid iGEM.2 with Golden Gate reaction using truncated ACT1 promoter

66 KB (11,234 words) - 01:40, 28 September 2013
Team:Heidelberg/Project/Delftibactin
...thereby avoid the intricacies expected for cloning of a single 59 kbp plasmid as well as to allow for faster trouble shooting in case issues with the clo <li>Plasmid: methylmalonyl-CoA pathway, PPTase sfp & permeability device <a href='http:

66 KB (9,751 words) - 03:53, 29 October 2013
Team:UGent/Labjournal
<li> General techniques: plasmid/PCR purification, inoculation, gel extraction, restriction & ligation. </li <li> Purify plasmid pTGD-ccdA-Pmb1GFP-CmFRT using Qiagen spin mini kit: Nanodrop --

45 KB (7,829 words) - 01:35, 5 October 2013
Team:KU Leuven/Journal/EBF/wetlab
... for the unsuccessfully transformed biobricks. Later we also performed the plasmid extraction on all the successfully transformed biobricks. ... 3 ml LB medium, then isolated the plasmid and cut the backbone out of the plasmid. To make sure that we have enough backbone we did this in quadruple. Before

64 KB (10,721 words) - 03:34, 29 October 2013
Team:Freiburg/Project/induction
...//2013.igem.org/Team:Freiburg/Project/crrna#rnaimer">RNAimer</a>. This plasmid codes for the RNAs that are necessary for the binding ability of dCas9. <td> Figure 1: Plasmid-cards of red-light effector-control plasmids 

46 KB (7,044 words) - 03:50, 29 October 2013
Team:Cornell/notebook
|tech=From the plasmid pUCATPH (pAh13G), we amplified the ''trpC'' promoter (P''trpC'', a constitu ... author flyouts I implemented yesterday, as well as other keywords such as plasmid names and protocols.

179 KB (31,118 words) - 03:34, 29 October 2013
Team:Freiburg/Project/crrna
... to be cotransfected with the dCas9-plasmids. Therefore we designed an RNA plasmid, termed <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/c ...ed using the iGEM BioBrick cloning strategy thereby allowing for a RNAimer plasmid carrying multiple crRNAs. 

35 KB (5,041 words) - 03:59, 29 October 2013
Team:Macquarie Australia/Notebook
...esults indicated that all the genes had similar intensities to the control plasmid and vectors, except for ChlM, which was slightly lower. ...ed. The results indicated that the gene was successfully inserted into the plasmid vector.

46 KB (7,422 words) - 03:48, 28 September 2013
Team:Macquarie Australia/Notebook2
...esults indicated that all the genes had similar intensities to the control plasmid and vectors, except for ChlM, which was slightly lower. ...ed. The results indicated that the gene was successfully inserted into the plasmid vector.

38 KB (6,401 words) - 02:25, 28 September 2013
Team:Goettingen/NoteBook w5
15 µl part 7 plasmid DNA (1 µg) 5 µl GFP-Term C1 plasmid DNA (1 µg)

121 KB (16,849 words) - 19:59, 30 September 2013
Team:Freiburg/Project/effector
...ps://2013.igem.org/Team:Freiburg/Project/crrna#rnaimer">RNAimer</a>, a plasmid coding for the required RNAs. By using several RNAs mutliple targeting is a ...ecting our <a id="link" href="http://parts.igem.org/Part:BBa_K1150034">RNA plasmid</a> which codes for the desired crRNA, dCas9-VP16 specifically binds to the

45 KB (6,905 words) - 03:49, 29 October 2013
Team:Hong Kong HKUST/notebook/mod3
Plasmid extraction for pCMV/myc/mito Digestion of pCMV plasmid extraction product with PstI-HF and NotI-HF 

26 KB (4,151 words) - 23:14, 27 September 2013
Team:IIT Delhi/Notebook
...estriction enzymes present in the lab are usable, we digested the isolated plasmid with the two enzymes separately and ran them on the gel. Following was the ... plasmid DNA. This plasmid was discarded and fresh colonies inoculated for plasmid isolation. 

18 KB (2,726 words) - 03:21, 28 September 2013
Team:ITU MOBGAM Turkey/labdiary
Both manual and Roshe plasmid isolation kit was applied to isolate the Terminator. However, low concentra Plasmid isolation was made to RBS and T7 Promoter by the kit and manually.

33 KB (5,137 words) - 20:58, 4 October 2013
Team:Frankfurt/Notebook/Protocol
==Plasmid Preparation== __NOEDITSECTION__ ===Plasmid Preparation from ''E.coli'' (Mini Preparation)=== __NOEDITSECTION__

12 KB (1,850 words) - 02:00, 5 October 2013
Team:Heidelberg/Templates/Materials list
| Plasmid Plus Maxi Kit (25) || QIAGEN || 12963 | Plasmid Plus Midi Kit (25) || QIAGEN || 12943

95 KB (12,744 words) - 07:35, 15 January 2014
Andrew Crouch
*Started a 1 L culture of EcNR2 with our plasmid with the pLtetO promoter in LB min + kanamycin + arabinose + ATC *Started an EcNR2 with our plasmid with pLtetO culture of 3ml LB with 3ul Kan

32 KB (5,157 words) - 13:43, 11 October 2013
Team:SYSU-China/Notebook/Labnotes
1. DNA extraction techniques: Plasmid extraction, DNA clean up(extraction from PCR or restriction endonuclease di Contents: First plasmid construction：pCDNA3.0-PGK-BSD

26 KB (4,093 words) - 04:21, 28 September 2013
Team:Marburg/Notebook:July
Check correct assembly of the plasmid for antibody production <li>1 µl Plasmid (pSB1A3-iBB10+iBB9, pSB1A3-iBB11+iBB9, pSB1A3-iBB12+iBB9 and pSB1A3-iBB13+i

43 KB (5,715 words) - 11:15, 27 October 2013
Team:Warsaw/Genetic lab journal
Isolate plasmid DNA by alkaline lysis. Quality checked with spectrophotomeric analysis with ... with mini-prep kit. Digest and ligation of construct: J23100 and B0034 on plasmid pSB1C3.

14 KB (2,179 words) - 17:28, 4 October 2013
Team:NTU-Taida/Notebook/Journal/August
#Ligation the three tubes overnight (plasmid undergo blue-white selection) ===Plasmid extraction===

14 KB (2,061 words) - 21:25, 27 September 2013
Team:INSA Toulouse/contenu/lab practice/notebook/protocols/backbone
<h3 class="title3">Plasmid Backbones</h3> ...This protocol was developed by Tom Knight. Samples of standard Registry plasmid backbones prepared using this method are sent out in the DNA Distribution k

12 KB (1,848 words) - 09:55, 9 September 2013
Team:UCSF/Project/Conjugation/Data
... conjugative genes integrated into the chromosome, and a small conjugative plasmid containing the origin sequence for conjugative transfer, pARO190. We were t ...es and selected for target strain cells that have received the conjugative plasmid, which we call “transconjugates”.

12 KB (1,757 words) - 02:00, 29 October 2013
Team:Peking/Project/BandpassFilter
...C3 as backbone. The regulator plasmid was co-transformed with the reporter plasmid, and then the activation curve was measured. (Fig. 13) ...id promoter Phyb and report gene sfGFP. The regulator plasmid and reporter plasmid use pSB4K5 and pSB1C3 as their backbone, respectively.

43 KB (6,197 words) - 20:39, 28 October 2013
Team:Goettingen/NoteBook w4
...span lang="EN-US">the other clones (linearized plasmid part 7 and linearized plasmid/5 µl<o:p></o:p>

212 KB (28,088 words) - 19:56, 30 September 2013
Team:SJTU-BioX-Shanghai/Notebook/Lab log/September
===Construct t7-lac inducible dCas9,pcyA and Ho1 plasmid=== Digest T7-pcyA-pRSFDUET plasmid and PCR product and then purify.

3 KB (417 words) - 04:17, 28 September 2013
Team:UCL/BacterialLabs
....org/Team:UCL/Project/Protocols" target="_blank"> competent cells</a> with plasmid YB3110 was carried out. ...CL/Project/Protocols" target="_blank"> Ampicillin plates</a> indicating no plasmid uptake. <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="

53 KB (9,305 words) - 23:54, 1 October 2013
Team:UCL/MammalianLabs
....org/Team:UCL/Project/Protocols" target="_blank"> competent cells</a> with plasmid YB3110 was carried out. ...CL/Project/Protocols" target="_blank"> Ampicillin plates</a> indicating no plasmid uptake. <a href="https://2013.igem.org/Team:UCL/Project/Protocols" target="

53 KB (9,308 words) - 09:18, 2 October 2013
Team:Freiburg/Notebook/lab light
<th> Plasmid </th> ...d 3 and 5 were send to GATC Bottom bands: pIG4101, expected sizes: Plasmid 4 and 7 were send to GATC </td>

55 KB (7,571 words) - 05:16, 28 October 2013
Team:Freiburg/Notebook/method
<h3> BbsI digest of RNA plasmid (from Hormon group) </h3> <h3> RNA-Plasmid </h3>

69 KB (10,135 words) - 19:50, 28 October 2013
Team:Heidelberg/Project/Indigoidine
...igure" title="Exchange of the indC T-domain on a pSB1C3 derived expression plasmid" href="https://static.igem.org/mediawiki/2013/c/ca/Heidelberg_IndPD_Fig2.pn ...Figure 2: Exchange of the indC T-domain on a pSB1C3 derived expression plasmid

60 KB (8,212 words) - 21:42, 17 October 2013
Team:Penn/AssayOverview
...mined to include standardized bisulfite sequencing primers on the MaGellin plasmid, so users would have the option of bisulfite sequencing after screening fun ...d specificity of any DNA binding domain – methylase fusion protein. This plasmid-based methylation assay is called MaGellin.

12 KB (1,807 words) - 02:49, 29 October 2013
Team:UCSF/Project/Conjugation/Data1
... conjugative genes integrated into the chromosome, and a small conjugative plasmid containing the origin sequence for conjugative transfer, pARO190. We were t ...es and selected for target strain cells that have received the conjugative plasmid, which we call “transconjugates”. On average, we obtained a conjugatio

23 KB (2,814 words) - 03:32, 29 October 2013
Team:Edinburgh/Project/Notebook
...te antibiotic. Following 7h incubation the entire culture was pelleted and plasmid was purified using Qiagen MiniPrep Kit. NanoDrop was used to measure the DN ...gation were resuspended in 5 ml LB medium + KAN and grown in 37 deg.C. The plasmid was purified following 4 hours of incubation using Qiagen MiniPrep Kit (Ale

41 KB (6,583 words) - 22:47, 4 October 2013
Team:Penn/MaGellinResults
...icity of any DNA binding domain – methyltransferase fusion protein. This plasmid-based methylation assay is called MaGellin. ...d.png" alt="MaGellin" width="700" height="395"><figcaption>The MaGellin plasmid includes all the features needed to clone, express, and assay site-specific

7 KB (1,129 words) - 07:10, 27 October 2013
Team:METU Turkey/notebook.html
...1, dh5α-A, dh5α-B, ? , will be taken at 9 and competent cell and plasmid isolation protocols will be started. - Plasmid isolation of K302033+I732820 3:1 is done. Nanodrop value is 35.6. It is in

68 KB (11,183 words) - 03:36, 5 October 2013
Team:Greensboro-Austin/Smell degradation
... for deodorization of ''p''-cresol. If successfully expressed, the ''pch'' plasmid alone would degrade ''p''-cresol to the effectively odorless intermediate, ===Plasmid Design===

16 KB (2,288 words) - 01:23, 26 October 2013
Team:Evry/Protocols
Thoses antibiotics are used to select bacteria with a plasmid including an interest construction and the corresponding antibiotic resista Transform each strain with a pSB1A3 plasmid (red colonies) and plated them on LB medium with Ampicillin only to evaluat

44 KB (6,816 words) - 03:48, 5 October 2013
Team:Bielefeld-Germany/Biosafety/Biosafety System L
...guarantees a high plasmid stability, which is extremely important when the plasmid contains a toxic gene like the [https://2013.igem.org/Team:Bielefeld-German ... growth of the Biosafety-Strain K-12 ∆''alr'' ∆''dadX'' containing the plasmid <bbpart>BBa_K741002</bbpart> with GFP (<bbpart>BBa_E0040</bbpart>) under th

37 KB (5,562 words) - 03:49, 29 October 2013
Team:Bielefeld-Germany/Biosafety/Biosafety System S
...guarantees a high plasmid stability, which is extremely important when the plasmid contains a toxic gene like the [https://2013.igem.org/Team:Bielefeld-German ...rowth of the strain K-12 ∆''alr'' ∆''dadX'' ∆''araC'' containing the plasmid <bbpart>BBa_I13541</bbpart> with GFP (<bbpart>BBa_E0040</bbpart>) under the

39 KB (5,873 words) - 02:46, 29 October 2013
Team:Calgary/Notebook/Journal/Linker
...d in our testing purposes. After successful transformation of the part the plasmid was isolated via miniprep and its presence was confirmed through a restrict ... overnight cultures were prepared on the weekend so that a miniprep of the plasmid could be performed the next week.

21 KB (3,481 words) - 03:53, 29 October 2013
Team:KU Leuven/Project/Glucosemodel/qPCR
...PCR reaction will not differentiate between the original copy (gene on the plasmid) and the cDNA (Reverse Transcribed from the mRNA). If the original copy is <h3>Plasmid DNA contamination</h3>

10 KB (1,620 words) - 03:06, 29 October 2013
Team:Evry/Protocols/
Thoses antibiotics are used to select bacteria with a plasmid including an interest construction and the corresponding antibiotic resista Transform each strain with a pSB1A3 plasmid (red colonies) and plated them on LB medium with Ampicillin only to evaluat

44 KB (6,791 words) - 02:37, 5 October 2013
Team:Evry/Protocols/Test
Thoses antibiotics are used to select bacteria with a plasmid including an interest construction and the corresponding antibiotic resista Transform each strain with a pSB1A3 plasmid (red colonies) and plated them on LB medium with Ampicillin only to evaluat

44 KB (6,791 words) - 02:35, 5 October 2013
Team:TU-Munich/Notebook/Methods
**QIAGEN Plasmid Midi Kit (Qiagen) ====Isolation of Plasmid DNA from ''E. coli'' (miniprep)====

46 KB (6,472 words) - 01:29, 29 October 2013
Team:Tsinghua-E/Notebook
... the information about primers was shown in “primer information”. This plasmid was named after pTrc99A_trp sensor_lacZ. ...D promoter (pBAD). We amplified pSC101-Cm-pBAD and ''sfGFP'' fragment from plasmid AraC_pBAD_CI_OR222-sfGFP, which was kindly provided by Professor Ouyang Qi

26 KB (4,157 words) - 17:51, 27 September 2013
Team:Tuebingen/Notebook/Journal/Weekly
...S315 has one additional EcoRI restriction site which was not marked in the plasmid map. ...and DNA purfications. Last weeks 3A-Assemblies had mixed results (found by plasmid preparation): Psuc2-mOrange and Pfet3-mOrange were negative (however, we pi

19 KB (3,243 words) - 14:51, 13 February 2014
Jamboree/Team Abstracts
... cerevisiae and Rhodotorula glutinis. Through the expression plasmid, yeasts are transformed from the wild-type phenotype into a thermogenic phe ... weapon, an M13 phage which it releases to infect other E. coli, injecting plasmid DNA into them. Finally, ninja must harmonize with the natural environment,

182 KB (27,381 words) - 14:33, 28 October 2013
Team:UNITN-Trento/Protocols
...te the cells (use plates with the appropriate antibiotic according to your plasmid).</li> strain – [resistance] – part – “(plasmid)” – YOUR_NAME – date 

40 KB (5,683 words) - 16:30, 3 October 2013
Team:Newcastle/Parts/HBsu-fp
...o the genome. We did not include a promoter in our BioBrick as the pMutin4 plasmid we used as a vector contained a Pspac promoter 5' to its multiple cloning s ... prefix and suffix from the pMutin 4 plasmid and cloned it into the pSB1C3 plasmid in order to be sent off to the iGEM registry.

43 KB (6,462 words) - 18:46, 28 October 2013
Team:Bielefeld-Germany/Labjournal/Molecular
...lation we used a [[Team:Bielefeld-Germany/Labjournal/Molecular#Used Kits | Plasmid Miniprep Kit]]. *Add 0.5-5 µL plasmid to 50 µl electrocompetent cells

48 KB (6,864 words) - 03:26, 29 October 2013
Team:KU Leuven/Journal/FFL/wetlab
The remaining 3.6 ml was used for a plasmid extraction using the home-made protocol. After the extraction we used the N ...C for usage in the next few weeks, namely B0015, B0034 and K608002. We did plasmid extraction of these and of the cells with GFP + terminator next day. We use

35 KB (5,899 words) - 21:24, 27 October 2013
Team:UANL Mty-Mexico/Safety/stability test
...ed to determine for how many generations can a cell culture keep a foreign plasmid in non-selective conditions. ... replication origin of each plasmid should be the main factor that affects plasmid stability.

7 KB (881 words) - 01:56, 29 October 2013
Team:Peking/Project/Plugins
After obtaining correct sequence of XylB and XylC via PCR from TOL plasmid pWW0 in Pseudomonas putida, we put XylC and XylB under the control of Pc pr ..., in which the oxidation of naphthalene has been extensively investigated. Plasmid pDTG1, NAH7 and pND6-1 identified in different P. putida strains all

42 KB (5,774 words) - 18:16, 28 October 2013
Team:BIOINT Mexico/Lab work
'''Experiment 3: Plasmid purification (July 16th, 2013)''' *The eppendorf tube contains the purified plasmid

41 KB (6,916 words) - 04:05, 28 September 2013
Team:York UK/Notebook.html
...t="mistake">So all of our parts that were to be put inside the iGEM plasmid only had XbaI and SpeI cutting sites, but we were unaware that they actuall ...vious PCR, should have used the genome) and pSB1C3 backbone(the linearised plasmid from iGEM HQ). Unfortunately, the backbone PCR failed.

51 KB (8,423 words) - 14:47, 21 October 2013
Team:SYSU-China/Project/Result/element test
...l experiment to compare their performance. The candidates were cloned into plasmid Plasmid E used in the experiment produced background GFP, which was a problem negle

21 KB (3,229 words) - 03:41, 29 October 2013
Team:Heidelberg/Delftibactin/MMCoA
...fy; position:relative; margin-left:6%;">After a validation of the template plasmid via restriction digest the PCR reaction was optimised. Unfortunately none o ... sfp could not be detected. Thus the entirely new strategy of generating a plasmid instead of doing genomic integration was initialised.

37 KB (3,119 words) - 09:42, 18 October 2013
Team:Heidelberg/Templates/Delftibactin Results
... were successfully established and optimized. At the same time, a separate plasmid was created encoding the PPTase from ''Bacillus subtilis'', the MethylMalon ... pattern expected from in silico digestion. Clones (6 and 9) contained the plasmid that encodes for the Methylmalonyl-CoA pathway (Fig. 16a). The obtained DNA

18 KB (2,605 words) - 19:56, 25 October 2013
Team:SCU China/Project
...lasmids. On the other hand, the G- cells were constructed by using another plasmid incompatible with F factor. ...ch could not divide any more but were capable of transferring or accepting plasmid.

36 KB (5,435 words) - 10:55, 27 October 2013
Team:Bielefeld-Germany/Labjournal/August
...gure 1: Agarosegel from gradient PCR on <bbpart>BBa_J04450</bbpart> pSB1C3 plasmid with forward and reverse primer pSB1C3 ''gldA'' to amplify pSB1C3 backbone **''gldA'' BioBrick plasmid (<bbpart>BBa_K1172201</bbpart>) was examined by [[Team:Bielefeld-Germany/La

40 KB (5,521 words) - 00:00, 29 October 2013
Team:NTU-Taida/Notebook/Journal/July
===Check and Plasmid DNA extraction (mini-prep):=== ===Check: (for plasmid ready to be sequenced)===

12 KB (1,777 words) - 21:22, 27 September 2013
Team:BYU Provo/Notebook/Cholera - Detection/Winterexp/Period2/Dailylog
...mL of our E.Coli overnight solution in our samples ... and at the end, our plasmid concentrations were low (24 ng/microL and 16ng/microL, respectively). We us ...d we need to find out why our E.coli doesn’t glow. When I understand the plasmid sequence, hopefully we can know what is missing or defective in our E. coli

16 KB (2,963 words) - 22:59, 27 September 2013
Team:CAU China/Protocal
==== 1. Gene and plasmid ==== (1) Plasmid Mini-preparation Kit (BioTeke);

18 KB (2,587 words) - 06:07, 27 September 2013
Team:Calgary/Sandbox/Notebook/Journal/Linker
...d in our testing purposes. After successful transformation of the part the plasmid was isolated via miniprep and its presence was confirmed through a restrict ... overnight cultures were prepared on the weekend so that a miniprep of the plasmid could be performed the next week.

17 KB (2,746 words) - 19:12, 16 September 2013
Team:British Columbia/Notebook/Flavours
...zation of each part. Our final construct should consist of a single pSB1C3 plasmid with all the genes inserted along with its ribosomal binding site and termi '''Aim:''' Digest 4-CL to see if 4-CL is present in the pSB1C3 plasmid with EcoRI and PstI

39 KB (6,228 words) - 03:33, 29 October 2013
Team:Bielefeld-Germany/Labjournal/September
...sites, were integrated in pSB1C3. Digestions were performed with 250 ng of plasmid-DNA + 2.5 µl of Cut-Smart-Buffer (NEB) + 0.5 µl of enzyme each. ...jpg|300px|thumb|center| '''Figure 3: Test-digest of the plasmid isolations with ''Eco''R1 and ''Spe''1 from NEB. Positive results gave lane

61 KB (8,793 words) - 00:02, 29 October 2013
Team:Bielefeld-Germany/Biosafety/Biosafety Strain
...cell death, but also loss of the alanine racemase (''alr'') encoded on the plasmid. ...upplementation or by complementation of the alanine racemase on a separate plasmid. As shown in [https://2013.igem.org/Team:Bielefeld-Germany/Biosafety/Biosaf

20 KB (2,920 words) - 09:44, 26 October 2013
Team:Goettingen/NoteBook w10
...="c10-6 c10-34">-  Sequencing of P3op C1: 6 µl of 175.7 ng/µl-plasmid solution + 1 µl iGEM 39 (VR) 1:5 --> tube 4</td></tr><tr><td class="c10-42">Part 7 C1 plasmid (ca. 12 ng/µl) or water for negative control</td><td class="c10

67 KB (12,672 words) - 21:18, 30 September 2013
Team:BGU Israel/Experiments
<li class="bulletlist">The C1 plasmid arrived from hy-labs and was diluted. Final concentration - 50 ng\ul.</li> <li class="bulletlist">kan cassete PCR from pkD4 plasmid ( T annealing= 52oC).</li>

71 KB (8,958 words) - 00:46, 29 October 2013
Team:BGU Israel/Solution
..., and it happened so that the lab we worked in had a stock of pGFPuv – a plasmid containing GFP gene under the regulation of lacO. So, we started conducting <h6>1. pUC57 plasmid</h6>

27 KB (3,790 words) - 01:36, 29 October 2013
Team:Goettingen/NoteBook w14
... followed by the exact volume of the sample that has to be used for 250 ng plasmid. This value is followed by the blue number indicating the volume that was a ...her reactions in case one or more of the components are contaminated with (plasmid) DNA

129 KB (20,625 words) - 21:12, 30 September 2013
Team:Heidelberg/Delftibactin/DelH
...o its large size of 18 kb, the module DelH will be expressed on a separate plasmid. A strategy was developed, primers designed accordingly and necessary BioBr ...as negative, so none of the transformed E.coli received the correct plasmid. 

54 KB (4,891 words) - 03:33, 29 October 2013
Team:BYU Provo/Notebook/CholeraDetection/Springexp/Period2/Dailylog
...sert. After 30 min, we did a transformation and inserted our newly ligated plasmid into DH5alpha. ... few colonies we had to singles. We can PCR-verify if any of them have the plasmid. Meanwhile I am going to redo the transformation today. I will plate 4 plat

7 KB (1,141 words) - 23:10, 27 September 2013
Team:Paris Saclay/Labwork
* Digestion of pSB1C3 plasmid and Pndh* PCR products by EcoRI/ PstI * DNA plasmid extraction of clones 5, 6

33 KB (5,021 words) - 00:59, 5 October 2013
Team:Heidelberg/Templates/Methods
===Plasmid-DNA isolation=== ...e and mixed by inverting 4-6 times. Lysate was then transfered to a QIAGEN Plasmid Plus spin column on the vacuum manifold. Vacuum was applied until all liqui

32 KB (4,951 words) - 01:39, 29 October 2013
Team:Goettingen/NoteBook w7
...-->Re-Trafo Clone 2 has a strange additional band (from plasmid?) ...ndif]-->Even for Plasmid of

138 KB (17,887 words) - 20:04, 30 September 2013
Team:Freiburg/Notebook/lab crrna plasmid
...ef="https://2013.igem.org/Team:Freiburg/Notebook/lab_crrna_plasmid"> crRNA-Plasmid </a> ...tps://2013.igem.org/Team:Freiburg/Notebook/lab_gfp_reporter"> GFP-reporter-plasmid </a> 

16 KB (2,227 words) - 05:12, 28 October 2013
Team:Bielefeld-Germany/Biosafety/Biosafety System
...e other and our Biosafety-System additionally provides additional a higher plasmid stability and a higher resistance towards undesirable mutations in the Safe ...ielefeld-Germany/Biosafety/Biosafety_System Biosafety-Strain] carrying the plasmid. And as the complementation is essential for bacterial cell division, it ca

24 KB (3,529 words) - 03:55, 29 October 2013
Team:UANL Mty-Mexico/Wetlab
<li><a href="#pcn">Plasmid copy number</a></li> <a name="pcn"><h3>Plasmid copy number</h3></a>

15 KB (2,292 words) - 02:57, 29 October 2013
Team:NJU China/Project
We cloned these three siRNAs into the vector pENTR/U6, which is a plasmid backbone for high yield of siRNA. ...nalysis of HepG2 cell co-transfected with both siRNA 467 plasmid and HBsAg plasmid, we observed the significant down-regulation of HBsAg gene (Fig.3). This de

57 KB (7,354 words) - 01:19, 29 October 2013
Team:NYMU-Taipei/Modeling/MainParts
N = number of plasmid in a single cell N = number of plasmid in a single cell

20 KB (3,021 words) - 02:40, 29 October 2013
Team:UChicago/Plan
...were unable to get any transformants. Prior to the transformation with the plasmid containing kerA, we tested the transformation efficiency of our competent c ...A in B. subtilis in high amounts, we need to use a high copy number plasmid. Since high copy number plasmids for B. subtilis are not available f

11 KB (1,796 words) - 03:12, 26 October 2013
Team:IIT Madras/Results
...ransformed into E.coli DH5α cells. Mini-prep was performed to isolate the plasmid. The gel image is shown in Fig 1.1 below. Fig 1.1: Plasmid Vector pSB1C3 isolation (3 kb each well)

8 KB (1,211 words) - 03:31, 28 September 2013
Team:Freiburg/plasmids
<tr> <th> plasmid name </th> <th> Description </th> <th> Backbone </th> <th> Resistance </th> <tr> <td> pIG0016 </td> <td> RNA Plasmid </td> <td> pSB1C3 </td> <td> Chloramph. </td> <td> </td> </tr>

62 KB (8,456 words) - 22:35, 4 October 2013
Team:NTNU-Trondheim/Notebook/August
...rnight. The plate had growth, indicating that the bacteria still had their plasmid. ...obrick <a href="http://parts.igem.org/Part:BBa_J04450"> BBa_J04450</a> (in plasmid with kanamycin resistance) was transformed into ''E.coli'' strain ER2566 ac

68 KB (11,155 words) - 20:56, 4 October 2013
Team:XMU-China/Content parts
This plasmid consists of two parts of target genes: gfp and luxI, and both of them are r <tr><td>Fig.7-1 AGE confirmation of plasmid pSB1C3-gfp-luxI construction</td></tr></table>

16 KB (2,735 words) - 19:06, 28 October 2013
Team:Braunschweig/Notebook
... in 2xYT medium containing respective antibiotics in order to miniprep the plasmid DNA tomorrow. We also inoculated overnight cultures for plasmid preparations the next day.

120 KB (18,005 words) - 13:15, 27 October 2013
Team:UC Chile/Biobricks
The large subunit of RuBisCO was obtained from the pHnCBS1D plasmid (plasmid provided by David Savage, Departments of Molecular & Cell Biology and Chemi The pHnCBS1D plasmid was provided by David Savage (Departments of Molecular & Cell Biology and C

20 KB (2,798 words) - 22:50, 27 September 2013
05/08/13
==Isolation of ligated plasmid== *The ligated plasmid- chloramphenicol backbone and limonene biobrick, were isolated from cells t

2 KB (321 words) - 12:30, 6 August 2013
Team:NTU Taiwan/index.html
...n Skunk cabbage.[5][6] We use SrUCPA gene as our material to construct our plasmid. ...tions quickly in E. coli, mutagenesis, and PCR.[1] Generally, these plasmid vectors contain genetic material derived from the E.coli, origin of

165 KB (19,258 words) - 04:22, 28 September 2013
01/08/13
==Digestion of the chlorophenicol plasmid vector pSB1C3 == ==Ligation of the plasmid pSB1C3 with the purified limonene biobrick (plasmid pSB1C3)==

3 KB (539 words) - 14:16, 5 August 2013
5 August 2013
==Isolation of ligated plasmid== *The ligated plasmid- chloramphenicol backbone and limonene biobrick, were isolated from cells t

2 KB (321 words) - 12:08, 2 September 2013
1 August 2013
==Digestion of the chlorophenicol plasmid vector pSB1C3 == ==Ligation of the plasmid pSB1C3 with the purified limonene biobrick (plasmid pSB1C3)==

3 KB (539 words) - 12:06, 2 September 2013
Team:SCU China/Notebook
#The linearized plasmid backbone, which is harvested by PCR，is a linear piece of DNA. It has a fe #The new composite part sample is ligated into the construction plasmid backbone at the ''EcoR ''I and ''Pst ''I sites.

6 KB (1,012 words) - 21:26, 27 September 2013
Team:UGent/Results
...pSB6A1), but failed multiple times for pSB1C3. We then tried to create the plasmid using the Gibson Assembly technique. This was done using T7-ccdB and For the construction of our plasmid we chose to work with a T7 promoter for the ccdB gene as to avoid le

21 KB (3,450 words) - 11:34, 15 October 2013
Team:Goettingen/NoteBook w9
...rts?; C4 insert has same bands as pBluescript RD, though bands of uncut C4 plasmid has different bands: No explanation…? Samples switched??? 1x C1 from transformation of 1 ng pBluescript plasmid (see above) (white epi B)-<spa

93 KB (17,934 words) - 21:05, 30 September 2013
Team:Marburg/Notebook:June
<legend><a name="lig">Ligation, Transformation, Plasmid prep</a></legend> <li>Insert und plasmid were transformed using a 1:2 ratio. Samples were incubated ON at RT.</li>

47 KB (6,413 words) - 11:14, 27 October 2013
Team:USTC CHINA/Notebook/Timeline
Introduce plasmid containing the GFP sequence into E.coli Extract the plasmid after verified by PCR 

9 KB (1,255 words) - 12:41, 28 October 2013
Team:UC Chile/Side Project
... a modified binary vector that we pretended to add to Parts Registry. This plasmid would have the sequences for TALENs, proteins that can recognize a specific ...f the T-DNA and had modified the vector that contains this sequence, the T-plasmid, with the objective of using this bacterium to generate transgenic plants.<

22 KB (3,097 words) - 01:02, 28 September 2013
Team:UC Chile/BioBricks
The large subunit of RuBisCO was obtained from the pHnCBS1D plasmid (plasmid provided by David Savage, Departments of Molecular & Cell Biology and Chemi The pHnCBS1D plasmid was provided by David Savage (Departments of Molecular & Cell Biology and C

20 KB (2,850 words) - 01:25, 13 October 2013
Team:XMU-China/Content exploration
1. Single Plasmid A1, B and C Confirmation by SDS-PAGE ...ut we assume it was the problem of Marker.) And pSB3T5-aiiA and pSB4K5-ndh plasmid do not have their target gene expressed without inducer AHL. 

14 KB (2,312 words) - 19:24, 28 October 2013
Team:BYU Provo/Notebook/Cholera - Detection/Winterexp/Period3/Dailylog
...mL of our E.Coli overnight solution in our samples ... and at the end, our plasmid concentrations were low (24 ng/microL and 16ng/microL, respectively). We us ...d we need to find out why our E.coli doesn’t glow. When I understand the plasmid sequence, hopefully we can know what is missing or defective in our E. coli

13 KB (2,394 words) - 22:12, 5 June 2013
Team:BYU Provo/Notebook/Cholera - Detection/Winterexp/Period1/Dailylog
...earch on what has already been done. One possible question is how to get a plasmid to form with medicine or something inside. Today I looked over existing plasmid constructs. Apparently iGEMers from last year did clone many genes into pla

24 KB (4,024 words) - 03:11, 23 September 2013
Team:BYU Provo/Notebook/SmallPhage/Cholera - Detection/Period4/Dailylog
...mL of our E.Coli overnight solution in our samples ... and at the end, our plasmid concentrations were low (24 ng/microL and 16ng/microL, respectively). We us ...d we need to find out why our E.coli doesn’t glow. When I understand the plasmid sequence, hopefully we can know what is missing or defective in our E. coli

11 KB (2,016 words) - 22:09, 5 June 2013
Team:BYU Provo/Notebook/CholeraDetection/Springexp/Period1/Dailylog
... for all the genes). We also have primers for working with CRO in the pBAD plasmid. ...ce Lambda into lysis through expression CRO. We will place CRO on the pBAD plasmid with the pBAD promoter (or on pLAT with a pBAD promoter), and the pBAD prom

8 KB (1,435 words) - 23:03, 27 September 2013
Team:KU Leuven/Protocols
<a href="#Isolation of plasmid DNA from E. coli (mini prep)">Isolation of plasmid DNA from E. coli (mini prep)</a> <a href="#Plasmid DNA isolation">Plasmid DNA isolation</a> 

38 KB (6,008 words) - 03:58, 29 October 2013
Team:Goettingen/NoteBook w2
bold">For Plasmid Mini-Preparation:Plasmid DNA<o:p></o:p>

119 KB (15,887 words) - 21:22, 30 September 2013
Team:Goettingen/NoteBook w3
...k to fold/extend">Digestion of PCR product (DarR Operator) (10 cycles) and Plasmid backbone (pSB1A3) cycles) and Plasmid backbone (pSB1A3 (with AmpR))<o:p></o:p>

143 KB (18,524 words) - 19:55, 30 September 2013
Team:Newcastle/HP/Ethics
... so homologous recombination can take place and the BioBrick move from the plasmid onto the genome. If one of these vectors did get into a bacteria with no re The L-forms switch BioBrick is on a pSB1C3 integration plasmid, which has homology to ''pbpb'' and ''murE''. Therefore the BioBrick would

15 KB (2,444 words) - 18:31, 2 October 2013
Team:TU-Munich/HumanPractice/Education
|"Banana-Odor"-Plasmid |"RFP-Generator"-Plasmid

12 KB (1,817 words) - 12:59, 30 December 2013
Team:Bielefeld-Germany/Biosafety/Biosafety System M
...guarantees a high plasmid stability, which is extremely important when the plasmid contains a toxic gene like the [https://2013.igem.org/Team:Bielefeld-German ... growth of the Biosafety-Strain K-12 ∆''alr'' ∆''dadX'' containing the plasmid <bbpart>BBa_K1172914</bbpart> with GFP (<bbpart>BBa_E0040</bbpart>) under t

33 KB (4,764 words) - 02:32, 29 October 2013
Team:Imperial College/Waste Degradation: SRF
*BioBrick assembly plasmid pSB1C3 is a high copy number plasmid (100-300 copies per cell)[http://parts.igem.org/Part:pSB1C3?title=Part:pSB1 *BioBrick assembly plasmid pSB1C3 is a high copy number plasmid (100-300 copies per cell)[http://parts.igem.org/Part:pSB1C3?title=Part:pSB1

109 KB (16,156 words) - 03:48, 29 October 2013
Team:Freiburg/Notebook/lab multiple targeting
...ef="https://2013.igem.org/Team:Freiburg/Notebook/lab_crrna_plasmid"> crRNA-Plasmid </a> ...tps://2013.igem.org/Team:Freiburg/Notebook/lab_gfp_reporter"> GFP-reporter-plasmid </a> 

54 KB (7,347 words) - 05:12, 28 October 2013
Team:Freiburg/Notebook/lab gfp reporter
...ef="https://2013.igem.org/Team:Freiburg/Notebook/lab_crrna_plasmid"> crRNA-Plasmid </a> ...tps://2013.igem.org/Team:Freiburg/Notebook/lab_gfp_reporter"> GFP-reporter-plasmid </a> 

23 KB (3,251 words) - 05:13, 28 October 2013
Team:Heidelberg/Templates/Indigoidine week18
!Plasmid!!Fragment 1!!Molarity [nM]!!Volume in ul!!Fragment 2!!Molarity [nM]!!Volume !Plasmid!!Fragment 1!!Molarity [nM]!!Volume [ul]!!Fragment 2!!Molarity [nM]!!Volume

9 KB (1,262 words) - 15:45, 21 October 2013
Team:Calgary/Notebook/Journal/Detector
...et sequences of TALE A and TALE B ([A] and [B]) in the RFP generator. This plasmid will be used as a target sequence. Primers were also designed that incorpor ...ormed. Subsequently, an overnight cultures of the colonies were made and a plasmid purification was performed. Digestion of the purified plasmids with NotI an

69 KB (12,089 words) - 03:40, 29 October 2013
Team:NJU China/Notebook
... We found that wrong antibiotics were added into the culture medium of RVG plasmid-containing E.coli cells (it ought to be kanamycin). So we did transferred E ...ected 4 flasks of 293T cells with 467 and 516 plasmids (467 plasmid*2, 516 plasmid*2).

65 KB (9,266 words) - 16:19, 28 October 2013
Team:Tsinghua/Lablog
...ntibiotic chloromycetin might be the one to blame. Next time when meet the plasmid with anti-chloro, be careful! ...se positive clones. I chose three positive clones per construct to extract plasmid on May 16th. There are 3 things to do for further work. First, do the seque

44 KB (6,950 words) - 20:43, 27 September 2013
Team:NJU China/Wet lab
... We found that wrong antibiotics were added into the culture medium of RVG plasmid-containing E.coli cells (it ought to be kanamycin). So we did transferred E ...ected 4 flasks of 293T cells with 467 and 516 plasmids (467 plasmid*2, 516 plasmid*2).

65 KB (9,266 words) - 01:22, 29 October 2013
Team:Freiburg/Project/truncation
...lso showed the expected length. We cut with NotI so that the insert of the plasmid is cut out. Only for truncation 4 we received a shorter fragment than expec ... 5 different truncated CMV:dCas9 constructs in pSB1C3 and the EMX1 RNAimer plasmid in 6-well plates. 42 hours post transfection cells were taken up in 500 µl

16 KB (2,472 words) - 03:24, 29 October 2013
Team:Penn/MethylaseCharacterization
...iseless chassis and because it's simpler to detect off target effects on a plasmid than a genome. ...specific methylation if 100% of the methylations is site-specific for that plasmid.</h7>

13 KB (1,929 words) - 03:23, 29 October 2013
Team:Leeds/Notebook
* ''Been a busy day; plasmid preps, glycerol stocks and further transformations of quorum sensing biobri * ''Another day of plasmid preps and innoculations in the lab of our newly transformed BioBricks.''

23 KB (3,580 words) - 03:52, 5 October 2013
Team:NTNU-Trondheim/Experiments and Results
 PCR for amplification of tat, plasmid backbone, GFP and RFP </div> ...FP, <a href="http://parts.igem.org/Part:BBa_J01101">BBa_J01101</a> for the plasmid backbone and genomic DNA from Escherichia coli strain ER2566 for the

28 KB (4,560 words) - 22:41, 4 October 2013
Team:CU-Boulder/Project/Kit/RestrictionEnzymes
...ry restriction endonucleases used in the Biobrick standard. Synthesizing a plasmid containing the gene for EcoRI and producing a simple purification method wo In order to synthesize a plasmid containing the desired genes, we digested EcoRI, its methylase, and a previ

11 KB (1,877 words) - 03:59, 29 October 2013
Team:Evry/Notebook/w7
<h2>Construction of plasmid N°1</h2> We make an electrophoresis with 5 µL of plamsid to check the plasmid purification made on the last friday.

20 KB (2,545 words) - 10:48, 7 September 2013
Team:SydneyUni Australia/Project/Results
...thene degradation (Varder & Wood, 2005), and gifted to our host lab in the plasmid pBS(Kan)ToMO.</li> <h4>Plasmid Preps </h4>

27 KB (4,029 words) - 01:55, 29 October 2013
Team:UGent/LiteratureStudy
...sing high or medium copy plasmids. However, studies have demonstrated that plasmid-bearing cells lose their productivity fairly quickly as a result of genetic ... years. However, the use of plasmids entails some important disadvantages: plasmid maintenance imposes a metabolic burden on cells and plasmids suffer from ge

21 KB (3,320 words) - 01:45, 5 October 2013
Team:Freiburg/Notebook/lab repression
...MV promoter were respectively co-transfected to HEK 293 cells with another plasmid, encoding CMV driven CNK. </li> <h3> Transfection of pIG2019 in combination with pKM006 (SEAP plasmid) and pSAM200 (SV40::tetR-VP16) </h3>

55 KB (8,278 words) - 05:15, 28 October 2013
Team:Freiburg/Notebook/lab epigenetics
 Clones were prepped with the Roche High Pure Plasmid Isolation Kit. Both clones were sent for sequencing with oIG8007 and oIG800 ...µl. A test digest was performed using EcoRV and NotI. If the correct plasmid is obtained, three bands will be visible (at 5850bp, 3400bp and 1700bp).

50 KB (6,849 words) - 05:15, 28 October 2013
Team:BYU Provo/Notebook/Cholera - Enzyme/September/Period1/Dailylog
...the E. coli cells in preparation to clone our biobrick parts into the iGem plasmid backbone for submission to the iGem registry. We prepared two minipreps and ...ll of the primers that we have been using and the new primers for the iGem plasmid backbone for both DspB and Savinase. This will allow us to see if the DNA t

7 KB (1,180 words) - 21:09, 27 September 2013
Team:NTU-Taida/Notebook/Journal/May
===Get Plasmid: Use Plasmid miniprep purification kit=== ===Get Plasmid:===

2 KB (290 words) - 21:46, 27 September 2013
Team:Hong Kong HKU/extra/diary
Tried to miniprep on tube with less clusters. No Plasmid. Mass Digestion of Tf native ppk plasmid 1,2 

21 KB (3,614 words) - 01:49, 28 September 2013
Team:SDU-Denmark/Tour51
...plasmid has the expected length around 5500 bp, and the EcoRI and PstI cut plasmid has expected lengths around 2000 bp and 3500 bp (Fig. 4). This, too, ...plasmid has the expected length around 5500 bp, and the EcoRI and PstI cut plasmid has expected lengths around 2000 bp and 3500 bp.">

15 KB (2,361 words) - 13:18, 8 January 2014
Team:SDU-Denmark/Tour34
...atural functions. If the bacteria were to survive in nature containing the plasmid, it would most likely be outcompeted by the naturally occurring and better ...="dialogLink" href="http://parts.igem.org/Part:pSB1A3">pSB1A3:</a> An iGEM plasmid backbone carrying an ampicillin resistance gene 

12 KB (1,860 words) - 01:12, 29 October 2013
Team:Uppsala/safety-experiment
...an experiment to see whether the engineered strain would lose the biobrick plasmid without competition over time. ...of cells carrying the biobrick plasmid. The fraction of cells carrying the plasmid declined rapidly, and already after 5 days (approximately 50 generations of

14 KB (2,007 words) - 16:02, 28 October 2013
Team:KU Leuven/Journal/MeS/qPCR
...ethyl salicylate MIT brick (2006; BBa_J45700)</li><li>We have the isolated plasmid DNA from this very same brick with a concentration of 155 ng/µl.</li><li>W ... false positive results. Therefore we ran a regular PCR, which amplifies a plasmid DNA region, to check if there is still any DNA contamination. Because all o

12 KB (1,851 words) - 17:40, 27 October 2013
Team:Tsinghua/Notebook-Protocol
For plasmid DNA propagation two bacterial strains were used: DH5alpha and TransT1. ... suitable antibiotics depending on the selection marker on the transfected plasmid: ampicilin 100 mg/L or kanamycin 50 mg/L.

19 KB (2,825 words) - 04:09, 28 September 2013
Team:Heidelberg/Templates/DelH overview25
...:File:Heidelberg_pFS_02.gb|pFS02 (pSB6A1_BBa_J23114_BBa_B0032_DelH) Gibson plasmid]].]]<div style="clear:both"></div> ...th a ccdB cassette flanked by KpnI and BamHI sites (pFS03). The final DelH plasmid will be assembled by restriction - ligation of pFS02 and pFS03 to pFS05.

4 KB (655 words) - 03:01, 29 October 2013
Team:Paris Bettencourt/Project/Detect
...porter element LacZ under the control of the pRecA promoter. As the helper plasmid doesn’t have a packaging sequence, only our sensor system phagemid is pac ...gene with the CRISPR/Cas system. <div style="font-size:90%">After the plasmid has been released into the target cells, gRNA and Cas9 get expressed. The g

31 KB (4,907 words) - 03:59, 29 October 2013
Team:Goettingen/NoteBook w0
"Arial","sans-serif"">Plasmid "Arial","sans-serif"">undigested plasmid | 

107 KB (14,511 words) - 08:19, 1 October 2013
Team:Freiburg/parts/favorite parts
...g this construct upstream of a promotor region. By co-transfecting our RNA plasmid [<a id="link" href="https://2013.igem.org/Team:Freiburg/Project/crrna#rnaim ..." href="https://2013.igem.org/Team:Freiburg/Project/crrna#rnaimer">RNAimer plasmid</a> (<a id="link" href="http://parts.igem.org/Part:BBa_K1150034">BBa_K11500

11 KB (1,666 words) - 03:41, 5 October 2013
Team:Heidelberg/Templates/DelH overview22
...:File:Heidelberg_pFS_02.gb|pFS02 (pSB6A1_BBa_J23114_BBa_B0032_DelH) Gibson plasmid]].]]<div style="clear:both"></div> ...th a ccdB cassette flanked by KpnI and BamHI sites (pFS03). The final DelH plasmid will be assembled by restriction - ligation of pFS02 and pFS03 to pFS05.

5 KB (761 words) - 02:58, 29 October 2013
Team:NTNU-Trondheim/Notebook/September
...tion of ''E.coli'' DH5α. The fragments that originated from the same plasmid where used together (BB from DT8 with tat_PrG from DT8 and so on). There wa ...late, giving in total four PCR batches. The first template was the Pm XylS plasmid, while the second was a PCR product of the Pm XylS promoter, modified in or

47 KB (7,543 words) - 20:57, 4 October 2013
Team:Heidelberg/Templates/Indigoidine week16
!Plasmid!!Fragment 1!!Molarity [nM]!!Volume in MM!!Fragment 2!!Molarity [nM]!!Volume ====Plasmid Validation====

15 KB (2,069 words) - 00:48, 5 October 2013
Team:ZJU-China/Notebook/TheElf/Design
...nduced promoter, an aptamer for atrazine, cheZ gene, RBS and GFP gene. The plasmid enables detectors to appropriately sense the atrazine gradient and move tow ...ter, a lux-induced promoter, RBS, luxR gene, luxI gene and ccdB gene. This plasmid is a gift from Dr. Lingchong You and Dr. Xuesong Zheng. The circuit aims to

2 KB (272 words) - 01:07, 29 October 2013
Team:UFMG Brazil/lab/results
... 2: plasmid digested with ''EcoR''I. 3: plasmid digested with ''Pst''I. 4: plasmid digested with ''EcoR''I and ''Pst''I.]] ...ure 6: Fluorimetric reads of cultures ''of E. coli'' XL1-Blue carrying the plasmid PSB1A3_RCNA+ YFP, after treatment with different concentrations of cobalt.'

10 KB (1,627 words) - 21:26, 27 October 2013
Team:SYSU-Software/tutorial
<li><a href="#part5">plasmid</a></li> 1. view the plasmid sequences 

8 KB (1,112 words) - 12:05, 28 October 2013
Team:Tokyo Tech/Project/M13 Shuriken
We constructed the plasmid for the system of inducible release of M13 phage (Fig. 2-2-2). In this syst ...s://2013.igem.org/Team:Tokyo_Tech/Experiment/pSB-M13_Plasmid_Assay pSB-M13 Plasmid Assay]).

14 KB (2,459 words) - 16:50, 28 October 2013
Team:BYU Provo/Notebook/CholeraDetection/Springexp/Period4/Dailylog
...roL of the plasmid pIG12 at a concentration of 116 ng/microL. This is the plasmid into which we'll be cloning CRO. ...he insert, waited an hour and a half, and then did a transformation of the plasmid into E.Coli to grow a lot of it. The E.Coli plates should be ready tomorrow

3 KB (562 words) - 03:19, 23 September 2013
Team:BYU Provo/Notebook/Cholera - Enzyme/March-April/Period1/Dailylog
...t it is working correctly. To help with this, we are creating a map of the plasmid to use as our reference for our sequencing. We have a generic map of the ge We continued working on our plasmid map today. There are seven genes that we are cloning into E. coli to allow

24 KB (3,572 words) - 21:04, 27 September 2013
Team:Penn/Notebook
...a T7 promoter activated by tetracycline, and the other would be a reporter plasmid to detect methylation. We completed our construct for our zinc finger fusion plasmid after a successful colony PCR and sequencing result. We performed our first

24 KB (3,801 words) - 02:08, 29 October 2013
Team:WLC-Milwaukee/Safety
livestock. We wanted to ensure that the plasmid could not be transferred to plasmid this is achieved by genome integrated CymR repression of the T5cumate promo

8 KB (1,215 words) - 03:06, 28 September 2013
Team:Northwestern/Notebook
...a transformation to test competency of cells prepared on 7/2 using a pUC19 plasmid. The transformations were tested on both Cb and Lb plates. Additionally, ou ...We also had an advisor’s meeting, where they suggested we use a low-copy plasmid for our construct, create a GANTT chart before next week, assign people to

26 KB (4,327 words) - 03:56, 28 September 2013
Team:Calgary/Sandbox/Notebook/Journal/Reporter
...essfully achieve the extraction of the beta-lactamase gene from the pSB1A3 plasmid. ...-lactamase from the <a href="http://parts.igem.org/Part:pSB1A3">pSB1A3</a> plasmid. These primers were used in three combinations to produce the beta-lactamas

23 KB (3,709 words) - 23:51, 22 September 2013
Team:Manchester/LabBookText
...asmid)) or the same volume of water/ligation mixture (control). ~1.2µl of plasmid to 50µl of cells The pKD46 plasmid we are using has temperature sensitive replication. Heat shocking and incub

26 KB (4,304 words) - 12:39, 30 September 2013
Team:Manchester/LabBooktest
...asmid)) or the same volume of water/ligation mixture (control). ~1.2µl of plasmid to 50µl of cells The pKD46 plasmid we are using has temperature sensitive replication. Heat shocking and incub

60 KB (8,597 words) - 13:33, 26 October 2013
Team:INSA Toulouse/contenu/lab practice/notebook/protocols/charac recomb
.... coli (selecting for the antibiotic resistance of the gate, hence the plasmid containing the recombinase is not selected). The bacteria colour gives the <li>Pre-culture overnight of the strain containing recombinase plasmid at 37°C.</li>

12 KB (1,848 words) - 01:35, 5 October 2013
Team:UGent/Experiments
...) and transferring this plasmid into the bacterial cell. In experiment 2 a plasmid containing ccdB (toxin) under control of a T7 promoter will be const ...verse mutation protocol, based on Gibson Assembly, to restore the original plasmid sequence. 

6 KB (924 words) - 01:47, 5 October 2013
Team:Paris Saclay/Notebook/August/27
====4 - Culture of strain MG1655Z1 Δfnr::Km containing plasmid pcp20==== ...liminate the Km cassette. Therefore, we can use this strain to receive the plasmid IGEM pSB1K3. Like this we will select clone Δfnr::Km by streaking them on

5 KB (859 words) - 01:20, 5 October 2013
Team:NYMU-Taipei/Experiments/Protocols
* Folding part: Plasmid Backbone ("A" for pSB1A2 or "C" for pSB1C3 or "X" for other) * 10am (2-3hrs): Plasmid extraction & Digestion

16 KB (2,543 words) - 03:33, 29 October 2013
Team:Peking/Project/BioSensors/XylS
...rchetype transcriptional activator of AraC/XylS family, mined from the TOL plasmid pWW0 of the bacterium Pseudomonas putida. It is composed of a C-term On TOL plasmid, the expression of XylS is initiated at two promoters: the strong promoter

32 KB (4,230 words) - 18:13, 28 October 2013
Team:Tokyo Tech/More/Collaboration
...lasmid due to out of stock. So team UT-Tokyo asked us for the plasmid. The plasmid is our past part of iGEM 2012. ...'t make usable plasmid. So team Kyoto asked us for the usable plasmid. The plasmid is our past part of iGEM 2009.

2 KB (332 words) - 18:00, 28 October 2013
Team:CAU China/Data
Transformation of pET-28a(+) for plasmid propagation Plasmid DNA isolation for pET-28a(+)

12 KB (1,691 words) - 07:39, 27 September 2013
Team:Calgary/Notebook/Journal/Reporter
...sfully achieve the extraction of the β-lactamase gene from the pSB1A3 plasmid. </a> plasmid. These primers were used in three combinations to produce the β-lactam

36 KB (5,736 words) - 03:56, 29 October 2013
Team:Freiburg/protocols
 <a href="#Plasmid Isolation"> Plasmid Isolation </a> <div id="Plasmid Isolation">

29 KB (4,493 words) - 05:11, 28 October 2013
Team:Alberta/Overview
...rigin city along any of the available roads: AmpR, ChlorR, or KanR. In the plasmid production, we begin with a combinatorial ligation – one where all three ...s the salesman might take next – three colour genes. Again, each growing plasmid can only incorporate one of the new genes, so we obtain a random assortment

21 KB (2,574 words) - 03:43, 29 October 2013
Team:USP-Brazil/GaTE
Plasmid BBa_E1010 that contains RFP gene was transformed into DH10B and plated on k Plasmid extraction successful was detected by running an agarose gel and its quanti

16 KB (2,551 words) - 01:25, 28 September 2013
Team:UniSalento Lecce/Protocols
<li>Resuspend the bacterial pellet in 4 ml or 10 ml (we use 15 ml for pGEM, plasmid at low copy number) Buffer P1.</li> <li>Add 4 ml or 10 ml (we use 15 ml for pGEM, plasmid at low copy number) Buffer P2, mix gently but thoroughly by inverting 4–6

40 KB (6,637 words) - 17:16, 4 October 2013
Team:UC Chile/Protocols
1. In an eppendorf tube, mix 1ng of plasmid DNA with 50μL of chemically competent cell on ice. Don’t leave the compe In an eppendorf tube, mix 50 ng of each plasmid DNA with 50μL of chemically competent cell on ice. 

40 KB (6,223 words) - 01:30, 13 October 2013
Team:Manchester/test4
...asmid)) or the same volume of water/ligation mixture (control). ~1.2µl of plasmid to 50µl of cells The pKD46 plasmid we are using has temperature sensitive replication. Heat shocking and incub

59 KB (8,384 words) - 11:41, 2 October 2013
Team:UCL/Labbook/Week8
...ttps://2013.igem.org/Team:UCL/Project/Protocols"> competent cells</a> with plasmid YB3110 was carried out. ....igem.org/Team:UCL/Project/Protocols"> Ampicillin plates</a> indicating no plasmid uptake. <a href="https://2013.igem.org/Team:UCL/Project/Protocols"> Transfo

6 KB (932 words) - 17:11, 4 October 2013
Team:Manchester/LabBook
...asmid)) or the same volume of water/ligation mixture (control). ~1.2µl of plasmid to 50µl of cells The pKD46 plasmid we are using has temperature sensitive replication. Heat shocking and incub

62 KB (8,850 words) - 13:33, 26 October 2013
Team:Gdansk-UG/Notebook
Plasmid </td> <td width="384" valign="top">Control (without plasmid)</td>

28 KB (3,102 words) - 16:41, 4 October 2013
Team:UFMG Brazil/Results
... 2: plasmid digested with ''EcoR''I. 3: plasmid digested with ''Pst''I. 4: plasmid digested with ''EcoR''I and ''Pst''I.]] ...ure 6: Fluorometric reads of cultures of ''E. coli'' XL1-Blue carrying the plasmid PSB1A3_RCNA+ YFP, along the time, after treatment with 0, 25, 50, 75, 100,

9 KB (1,436 words) - 01:08, 29 October 2013
Team:Glendale CC AZ/Notebook
.../2013.igem.org/Team:Glendale_CC_AZ/Protocols/AlkalineLysis">Alkaline Lysis Plasmid Miniprep </a> Lysis Plasmid Miniprep on sample B of single parts

37 KB (3,654 words) - 02:33, 28 September 2013
Http://2013.igem.org/Http://2013.igem.org/Team:TU-Delft/LabWork/Protocols
...a to prepare glycerol stocks and carry out Miniprep to extract the desired plasmid. ...e glycerol stocks and carry out a miniprep protocol to extract the desired plasmid from the bacterial cells.

10 KB (1,540 words) - 14:50, 19 August 2013
Team:Evry/Notebook/w9
...ium (with carbenicillin) have been launched for Top10 transformed with our plasmid which contains the sfGFP under the control of Lac O promoter. After one nig ...re without plasmid) and a positive controle (transformation procedure with plasmid 1A3) are made.

25 KB (3,160 words) - 10:25, 28 August 2013
Team:DTU-Denmark/Notebook/19 July 2013
* plasmid isolation * gel electrophoresis to verify insert after colony PCR (AMO, HAO), plasmid isolation

4 KB (549 words) - 11:35, 4 October 2013
Team:Evry/Constructions
<li><a href="https://2013.igem.org/Team:Evry/Constructions/01">1st Plasmid with GFP</a> <li><a href="https://2013.igem.org/Team:Evry/Constructions/02">1st Plasmid with LacI</a>

1 KB (205 words) - 09:31, 9 September 2013
Team:Glendale CC AZ/Notebook/Calendar
.../2013.igem.org/Team:Glendale_CC_AZ/Protocols/AlkalineLysis">Alkaline Lysis Plasmid Miniprep </a> 2. Performed Alkaline Lysis Plasmid Miniprep on sample B of single parts (LacI promoter, RBS, LEA, TT, and the

29 KB (3,585 words) - 00:06, 23 September 2013
29/07/13
==Digestion of plasmid backbone (pSB1C3)== ==Digestion of the limonene biobrick plasmid backbone (BBa_K118025)==

3 KB (424 words) - 13:57, 1 August 2013
Team:INSA Toulouse/contenu/lab practice/results/logic gates
...ontaining the gate to be switched. After incubation, transformation of the plasmid containing the gate allows the quantification of switched versus non switch ...th the two recombinases. The XOR1 gate was tested with the Dual Controller Plasmid provided by Bonnet (KC529324) and containing Tp901.1 and Bxb1. No switch wa

17 KB (2,615 words) - 15:29, 20 November 2013
Team:KIT-Kyoto/Notebook/YJL
Plasmid DNA Plasmid DNA was

80 KB (12,107 words) - 08:46, 27 September 2013
Team:British Columbia/Project/CRISPR
..., will eliminate the antibiotic resistance conferred by the “invading” plasmid, thereby resulting in cell death. These two plasmids contain the spacer and ...t on changes occurring in the host background (Figure 9). Furthermore, the plasmid sequences showed that the repeat-spacer regions was not present, showing th

18 KB (2,696 words) - 02:22, 29 October 2013
Team:Freiburg/Notebook/lab activation
 HEK293T cells were co-transfected with the following plasmid combination reporter plasmid (SEAP) : effector plasmid (Cas9) : RNA plasmid 

28 KB (4,392 words) - 05:14, 28 October 2013
Team:Peking/projecttest
...ption of the degradation genes in response to the inducer salicylate. This plasmid encodes enzymes for the metabolism of naphthalene or salicylate as the sole ... promoter with the reporter gene sfGFP (Fig. 5) via standard assembly. The plasmid verified by Beijing Genomics Institute was transformed into E. coli (TOP10,

23 KB (3,135 words) - 07:50, 17 September 2013
Team:Wageningen UR/Experimental protocols
... dNTPs (10mM), 1.25 µl MgCL2 (25mM), 0.05 µl Dream Taq polymerase, 50 ng plasmid DNA, and filled the rest with Milli-Q H2O untill 20 µl. Plasmid DNA are generally linearized by digesting with the selected restriction enz

35 KB (5,505 words) - 03:56, 5 October 2013
Team:EPF Lausanne/Sensing-Effector
The idea of this module was to transform bacteria with a plasmid that contains a promoter which senses a specific signal. Once this promoter ...hat the sensing module contained. Each promoter was inserted into the same plasmid in front of GFP]]

19 KB (3,055 words) - 18:11, 27 October 2013
Team:Imperial College/BioPlastic Recycling: PHB
...ever the plasmid is low copy and also the presence of the xylR gene on the plasmid definitely alters the expression level. Data fitted to Hill equation. Thi *BioBrick assembly plasmid pSB1C3 is a high copy number plasmid (100-300 copies per cell)[http://parts.igem.org/Part:pSB1C3?title=Part:pSB1

76 KB (11,472 words) - 03:55, 29 October 2013
Team:NCTU Formosa/notes
====Minipreps of Plasmid DNA==== ... DNA is purified by silicone resin-based column. The quality and amount of plasmid DNA is estimated by agarose gel electrophoresis stained by SyBr Safe (Invit

156 KB (21,473 words) - 03:58, 28 September 2013
Team:Lethbridge/results
...s of E. coli DH5α containing either the PK401 plasmid or an empty control plasmid were grown from glycerol stocks overnight at 37°C in 50 mL LB media contai ...nstruct. E. coli DH5α cells containing the PK401 plasmid or a control plasmid were grown at 37°C in LB media. The OD600 was monitored and the cultures w

14 KB (2,168 words) - 22:48, 21 January 2014
Team:Tuebingen/Project/Plasmids
...three plasmids that will be be transformed into S. cerevisiae. Each plasmid will contain one part of our project - receptor, inverter, reporter - where ...989) thus plasmid extractions from E. coli yield high quantities of plasmid DNA that can be used for yeast transformations.

4 KB (522 words) - 20:23, 3 October 2013
Team:UANL Mty-Mexico/Synthetic Rally
Stop #1: Plasmid Conjugation! Objective: Understand the plasmid conjugations in bacteria as a mechanism of natural DNA transference between

20 KB (3,418 words) - 01:47, 29 October 2013
Team:XMU-China/Content5
1.3 Plasmid (Biobrick) Plasmid

56 KB (7,188 words) - 18:00, 26 October 2013
Team:UniSalento Lecce/Notebook
...iotics. Colonies on construct n.6 plate are red, so we have to rebuild the plasmid from the digestion of the single construct (the same for nikR, pint, 1, 5, ...INVITROGEN T4 DNA LIGASE + 5X BUFFER; final volume: 10 ul; 2 ul per part + plasmid [10 ng/ul] + 2 ul buffer + 1 ul ligase).",

47 KB (6,595 words) - 14:03, 4 October 2013
Team:HUST-China/Modelling/MCOS
...r with plasmid copies. Given a specific environment, the number of initial plasmid copies are a constant. The replication of plasmids can be thought to be com (6)AraC's concentration is proportion to plasmid copies. 

14 KB (1,802 words) - 03:55, 29 October 2013
Team:IIT Madras/Weekly
...ic DNA from GenScript, Hong Kong. The construct was transported in a pUC57 plasmid Vector: The plasmid vector was digested with EcoRI and PstI. We wanted to check the successful

26 KB (3,593 words) - 23:55, 27 September 2013
Team:UC-Santa Cruz/Notebook2
the electroporation protocol with water instead of plasmid. This was just "Times New Roman";color:black'>Plasmid Mini-prep of E. coli cultures<span

167 KB (22,154 words) - 03:34, 28 September 2013
Team:Heidelberg/Templates/DelH week17
===Characterization of DelH Plasmid pHM03 17-08=== ..._PHM04-DelH-pSB6A1(without mRFP).gb|PHM04-DelH-pSB6A1(without mRFP) Gibson plasmid]].]]

25 KB (3,451 words) - 13:48, 25 October 2013
Team:EPF Lausanne/Next steps
...ater with the enzymes produced by the E.coli transformed with the effector plasmid. ...idin was exported to the outer membrane. The next step would be to clone a plasmid that encodes a fusion protein between Inp, streptavidin and YFP. Then, we w

4 KB (657 words) - 23:57, 28 October 2013
Team:Heidelberg/Templates/DelH week21
===Generation of DelH Plasmid pHM04 15-09=== ===Generation of DelH Plasmid pHM04 18-09===

29 KB (4,287 words) - 21:31, 25 October 2013
Team:Heidelberg/Templates/DelH week19
===Characterization of DelH Plasmid pHM04 30-08 Clone 12=== ===Characterization of DelH Plasmid pHM04 30-08 Clones 4, 6, 15===

15 KB (2,021 words) - 21:44, 25 October 2013
Team:Heidelberg/Templates/Indigoidine week19
For further evaluation of the single plasmid strategy we admired a plasmid with bpsA instead of indC as indigoidine synthetase. For a quick shot we wa ...fact that with assembly of pKH4 the BamHI cutting site was removed and the plasmid was only linearized by the KpnI cutting site. For pRB7 two bands at almost

12 KB (1,856 words) - 15:48, 21 October 2013
Team:NTNU-Trondheim/Notebook/July
...BS (<a href=" http://parts.igem.org/Part:BBa_J61101">BBa_J61101</a>) and a plasmid backbone (<a href=" http://parts.igem.org/Part:BBa_J01101">BBa_J01101</a>) ....coli'' BW27784 transformed with the pUM9 plasmid were preprared. The pUM9 plasmid has ampR induces a stress respons in ''E.coli'' when arabinose i

45 KB (7,110 words) - 20:56, 4 October 2013
Team:Evry/Modelmeta2
<li>We can answer the question "Which plasmid's copy should we prioritize in our bacteria?"</li> "Which plasmid's copy should we prioritize in our bacteria?" 

5 KB (847 words) - 03:41, 29 October 2013
Team:Goettingen/Parts
...formed with [http://partsregistry.org/Part:BBa_B0034 BBa_B0034(an RBS)] in plasmid Backbone pSB1A2. Subsequently, we characterized three clones of each strain ...ol strain did not fluoresce, while all strains transformed with a promoter plasmid exhibited a red fluorescence. The cells with promoter 1 and promoter 2 seem

63 KB (8,351 words) - 11:51, 4 October 2013
Team:Greensboro-Austin/Plasmids and Strains
|Use with IGM050 to amplify rest of plasmid without illegal PstI Sites = Plasmid Database =

11 KB (1,593 words) - 23:06, 9 September 2013
Team:DTU-Denmark/Project
...asmid for Mutant 1 should be the following which includes all genes on one plasmid: ...were able to [[Team:DTU-Denmark/Experiment4|verify that the AMO containing plasmid is working as designed]].

9 KB (1,257 words) - 22:04, 4 October 2013
Team:Newcastle/Parts/l form switch
The synthesised BioBrick was then inserted into the pSB1C3 plasmid - the only plasmid acceptable by iGEM for part submission. ...formed] into ''B. subtilis''. Integration of the BioBrick from the vector plasmid to the host chromosome was facilitated through homologous recombination wit

20 KB (2,990 words) - 18:35, 28 October 2013
Team:Evry/Notebook/w13
<h2>Plasmid 3</h2> Plasmid 3

25 KB (3,477 words) - 10:49, 1 October 2013
Team:Grenoble-EMSE-LSU/Documentation/Notebook/June
...e: since M15 cells were used last time and contain the pREP4 plasmid, this plasmid may prevent isolation of other plasmids of similar sizes during electrophor ...ssion, this plasmid makes more so that repression is efficient). The pREP4 plasmid confers kanamycin resistance. 

16 KB (2,369 words) - 01:06, 5 October 2013
Team:Freiburg/Project/method
..." href="https://2013.igem.org/Team:Freiburg/Project/crrna#rnaimer">RNAimer plasmid</a> HEK-293T cells are taken up in dilution buffer and lysed by sonifying. ...lasmid or co-transfected with dCas9 fusion constructs and the EMX1 RNAimer plasmid. Lysates were split and analyzed on uniBAss and western blot 42 h after tra

29 KB (4,418 words) - 03:30, 29 October 2013
Team:Kent/Project
...ns of ''E. coli'': ''norR'', ''norVW'' and NrfA. Our idea is to engineer a plasmid to that will express the nitrite reductase enzyme ''nrfA'' in an NO-depende ... The sequences corresponding to ''nrfA'' and ''norV'' were put into pSB1C3 plasmid. Below is a link to the PDF containing detailed results of our project, inc

14 KB (2,294 words) - 17:49, 4 October 2013
Exeter/16 August 2013
Miniprep of our complete Cph8 for inertion into CAM plasmid to be sent into the registry. Realised we had ran out of CAM plasmid, therefore had to transform some more out of the kit plate.

3 KB (410 words) - 20:24, 2 October 2013
Team:UNIK Copenhagen/Notebook
Overnight liquid cultures of pBBR1MCS-2 and eGFP plasmid (from Adam Takos) Cloning and transformation of eGFP-pBBR1MCS-2 using plasmid no. 4 (mentioned just above). The transformation only gave rise to 2 coloni

27 KB (4,144 words) - 22:15, 3 October 2013
Team:UT-Tokyo/Project
... <a href="http://parts.igem.org/Part:BBa_K1124112">BBa_K1124112</a> on the plasmid backbone pSB1C3, pSB1A3, or pSB1AK3. ...cedure 5) Cut the PCR product by Xba1 and Pst1, and the promoter part on a plasmid backbone by Spe1 and Pst1. Ligate these digests and you will get the full c

33 KB (5,129 words) - 05:40, 13 October 2014
Team:TU Darmstadt/labbook/Biobricks
<td>Plasmid prep of pSB1C3[LssmOrange] clones and pSB1C3[mKate] clones</td> <li>using PureYield Plasmid Miniprep System (Promega)<ul>

40 KB (2,780 words) - 03:16, 5 October 2013
Team:Uppsala/vectors
A shuttle vector is a plasmid that can be transferred between two different species and is able to replic ...d known to work in both E. coli and Lactobacillus. pSH71 originates from a plasmid, pJP059, from Lactococcus lactis but it is well known to replicate in both

13 KB (1,822 words) - 21:24, 28 October 2013
Team:UGA-Georgia/Plan
...n be properly expressed in the archaea ''Methanococcus maripaludis'' via a plasmid shuttle vector. Successful transformation will result in ''Methanococcus ma 1. Our first objective was to create a plasmid shuttle vector that would effectively express a gene for geraniol synthase

3 KB (414 words) - 17:44, 27 September 2013
Team:Bielefeld-Germany/Labjournal/July
...erent fragments will be ligated back together and into the pSB1C3 shipping plasmid via Gibson Assembly. **Further work on ''gldA'' BioBrick is needed: Plasmid [[Team:Bielefeld-Germany/Labjournal/Molecular#Restriction analysis|restrict

19 KB (2,676 words) - 23:58, 28 October 2013
Team:Minnesota/Project/Pichia Expression System
...o yeast. Transformation using TKC shortens the process by transferring the plasmid directly into the yeast cell. Utilizing TKC as a transformation protocol wo ...red to other variations of OriT- does not require the presence of a helper plasmid within the recipient to complete the final ligation step of conjugal transf

14 KB (2,163 words) - 02:17, 28 September 2013
05/09/13
==Isolate plasmid from overnight broth== The plasmids were isolated using the Omega Plasmid Mini Kit 1, and its protocol was followed.

3 KB (351 words) - 09:35, 11 September 2013
Team:Paris Saclay/Notebook/August/2
Tranformation from 07/30/13 works. We will extract plasmid BBa_K1155003. Protocol : [[Team:Paris_Saclay/extraction|High-copy plasmid extraction]]

3 KB (409 words) - 21:15, 4 October 2013
Team:BYU Provo/Notebook/CholeraDetection/Fallexp/Period2/Dailylog
...d the Ydiv gene into the iGEM backbone to submit the part; we purified the plasmid for submission. ...lera's AHL, and it controls the YdiV promoter. We will transform the YdiV plasmid into E.Coli and plate it next to cholera, expecting to see expression of GF

6 KB (1,001 words) - 22:34, 27 September 2013
Team:HZAU-China/Safety/Security Evaluatio
...family:arial, sans-serif;">Shuttle vector, namely pht304, phy300, and pma5 plasmid. <h3>2 The stability of plasmid and the degree of the potential risk </h3>

16 KB (2,468 words) - 04:09, 28 September 2013
Team:TzuChiU Formosa/Protocol
<li>Add 10 ng plasmid into the 200 ul competent cell. <li>Do a nanodrop test on the plasmid we want to cleave.

14 KB (1,938 words) - 00:01, 28 September 2013
Team:BGU Israel/Bricks
...k">Bba_K1223008</a></td> <td class="bb">pUC57-P.A.S.E.2</td><td class="bb">Plasmid</td> </tr> ...23009</a></td> <td class="bb">pUC57 backbone (REVERSED)</td><td class="bb">Plasmid Backbone</td> </tr>

25 KB (3,830 words) - 02:15, 29 October 2013
Team:BYU Provo/Notebook/CholeraDetection/Summerexp/Period5/Dailylog
...fied the PCR of RecA and cleaned it up. We were out of pIG12, so we did a plasmid prep of that and a digestion of the two. ...tion and then assay many colonies, we could find one that has taken up the plasmid AND remains vulnerable to lambda. We performed an electroporation today; i

6 KB (1,040 words) - 06:45, 23 September 2013
Team:XMU-China/Content3
<td rowspan=2>Plasmid</td> ...to degrade AHL and acts as the negative feedback in oscillator; the plasmid C expresses NAD-2 to generate H2O2 to communicate between colonies, is the

56 KB (9,034 words) - 01:54, 26 October 2013
Team:SCUT/Project/Oscillating odorant
Plasmid construct ...nes to help correctly folding the protein. That’s why we choose low copy plasmid, however, the diacetyl output is satisfied according to our results shown b

16 KB (2,465 words) - 03:51, 28 September 2013
Team:Stanford-Brown/Projects/CRISPR
...on of the RP4 plasmid. Electrocompetent cells were transformed with RP4, a plasmid coding for proteins needed for conjugation, and grown in liquid culture con | Figure 1. Mating experiment to prove cells harboring RP4 plasmid can conjugate. 

8 KB (1,163 words) - 03:31, 29 October 2013
Team:Alberta/Results
... ligations on the bead-bound strands. These are necessary for building the plasmid "routes", as described in the project overview page. ..., to eliminate the possibility that some interfering piece of the original plasmid was competing with our desired reaction. Finally, we attempted to increase

29 KB (3,720 words) - 03:45, 29 October 2013
Team:Freiburg/Project/attributions
<!--We used the pX334a plasmid (Addgene) provided by the Zhang lab. This plasmid contains a human codon optimized Cas9 with one mutated nickase function. In ...crRNA and crRNA loci which we amplified for further cloning of the RNAimer plasmid.

5 KB (903 words) - 02:36, 29 October 2013
Team:KU Leuven/Journal/MeS/wetlab
<li>lane 2: the original plasmid containing aroG</li> <li>lane 3: the original plasmid containing aroG cut with EcoRI to make it linear</li>

20 KB (3,201 words) - 03:22, 29 October 2013
Team:HUST-China/Protocol/Part1
...gene digested with EcoR I and SpeI, following that we loaded the 2118CA+hp plasmid in the agarose gel electrophoresis. The final image was displaced in fig1-4 Fig1-4 Verification of 2118CA+hp plasmid(1.2118CA+hp 2，1，O，M5000)

17 KB (1,843 words) - 16:16, 28 October 2013
Team:Tokyo-NoKoGen/Protocol
<h3>Inserting plasmid into E. coli</h3> 2; Add 1~5 μL of plasmid to competent cell (DH5α) on the ice. 

25 KB (3,659 words) - 01:57, 28 September 2013
Team:Hong Kong HKUST/experiment/exp2
...gin-left:130px;margin-right:130px;">Figure 1: Degradation of BBa_176171 plasmid at temperature above 50°C after denaturation step of polymerase chain reac ...nd HepG2 cell lines. GFP signal of the construct was compared with pEGF-N1 plasmid that contains constitutive CMV promoter. However, no GFP signal could be de

15 KB (2,217 words) - 23:15, 27 September 2013
Team:NJU China/Protocol
...and to minimize cytotoxicity, Optimization may be necessary(see Optimizing Plasmid DNA Transfection). Optimizing Plasmid DNA Transfection

69 KB (9,899 words) - 08:26, 28 October 2013
Team:Hong Kong HKUST/experiment/exp4
...e assembled separately in different plasmid before being combined into one plasmid. For ACEA construct, we decided to use a commercial plasmid called pShooter/myc/mito (Invitrogen). The vector is designed for ex

13 KB (1,944 words) - 13:06, 28 October 2013
Team:UFMG Brazil/lab/protocols
<td>Plasmid (PSB1A3)</td><td>10<html>μ</html>L</td><td>2<html>μ</html>L</td><td>2 C) TorCAD and Chloramphenicol plasmid resistance (PSB1C3)

21 KB (2,780 words) - 13:02, 27 October 2013
Team:UI-Indonesia/July
</li><li>Store the remaining suspended plasmid </li><li>Transforming the plasmid into E.coli top 10 cells

7 KB (1,076 words) - 02:41, 19 October 2013
Team:NJU China/test
...and to minimize cytotoxicity, Optimization may be necessary(see Optimizing Plasmid DNA Transfection). Optimizing Plasmid DNA Transfection

72 KB (10,248 words) - 02:00, 28 September 2013
Team:Nevada/Notebook/Month4
Christian did a transformation n for Cody’s submission plasmid. He also started a small culture of TOP 10 cells for assay. Christian, Behdad, Jasmine, and Jon worked on finishing the submission plasmid portion of the project.

4 KB (581 words) - 03:54, 28 September 2013
Team:EPF Lausanne/Cell surface display
...1C3 The three biobricks were cloned into the standard iGEM backbone plasmid, which contains a chloramphenicol resistance for selection. ...econd verification is that the Gibson product could still contain parental plasmid, whose insertion in cells can also lead to cells resistance and survival. S

27 KB (4,108 words) - 23:41, 28 October 2013
Team:Uppsala/toxin-antitoxin-system
...to use antibiotics and antibiotic resistance. If a clone were to lose the plasmid, the toxin which usually has a longer half life than the antitoxin will kil ...3_anti-toxin-toxin-system.jpg" data-lightbox="roadtrip"><img class="method-plasmid" src="https://static.igem.org/mediawiki/2013/d/dc/Uppsala2013_anti-toxin-to

11 KB (1,441 words) - 21:28, 28 October 2013
Team:UNIK Copenhagen/Notebook/Printable
Overnight liquid cultures of pBBR1MCS-2 and eGFP plasmid (from Adam Takos) Cloning and transformation of eGFP-pBBR1MCS-2 using plasmid no. 4 (mentioned just above). The transformation only gave rise to 2 coloni

23 KB (3,498 words) - 22:19, 3 October 2013
Team:Heidelberg/Delftibactin/DelRest
...t. Creating a 32 kb plasmid.</h1> ... and DelL-P (note: the 18 kbp gene encoding DelH is cloned onto a seperate plasmid). Due to the shere size and complexity of the DelRest construct, we decided

48 KB (4,394 words) - 01:37, 29 October 2013
Team:Frankfurt/Project/Description
... year's competition which gave us the possibility to transfer a mevalonate plasmid into yeast to increase the production of a steviol-precursor: geranylgerany ...rometry. For this reason, the necessary enzymes (encoded on the mevalonate plasmid) are expressed in yeast and the produced geranylgeranyl-pyrophosphate is is

3 KB (436 words) - 01:47, 5 October 2013
Team:Heidelberg/Templates/DelH week8
===Generation of DelH plasmid 19-06=== ===Characterization of DelH plasmid 19-06===

7 KB (953 words) - 08:12, 24 October 2013
Team:Heidelberg/Templates/DelH week15
===Generation of DelH Plasmid 01-08=== ===Generation of DelH Plasmid 09-08===

24 KB (3,497 words) - 13:11, 25 October 2013
Team:Heidelberg/Templates/DelH week16
===Generation of Plasmid DelH 11-08=== :=> None of the colonies harbours DelH plasmid.

14 KB (2,006 words) - 13:17, 25 October 2013
Team:UC Chile/Formation
...aboration of David Savage, from California Berkeley University. Since this plasmid is optimized for the heterologous expression of this microcompartment, we d ...stem is working correctly. Finally, if we induce the Carboxysome formation plasmid, again we should see GFP colocalized with the BMC, therefore this GFP would

21 KB (3,045 words) - 01:37, 13 October 2013
Team:XMU-China/Content mechanism
<td rowspan=2>Plasmid</td> ...to degrade AHL and acts as the negative feedback in oscillator; the plasmid C expresses NAD-2 to generate H2O2 to communicate bet

13 KB (2,052 words) - 17:53, 28 October 2013
Team:XMU-China/Content circuit
<td rowspan=2>Plasmid</td> ...to degrade AHL and acts as the negative feedback in oscillator; the plasmid C expresses NAD-2 to generate H2O2 to communicate bet

7 KB (1,050 words) - 17:44, 26 October 2013
Team:UFMG Brazil/Protocols
<td>Plasmid (PSB1A3)</td><td>10<html>μ</html>L</td><td>2<html>μ</html>L</td><td>2 C) TorCAD and Chloramphenicol plasmid resistance (PSB1C3)

15 KB (2,384 words) - 23:17, 28 October 2013
Team:Manchester/Notebook
...d things were really starting to come together. We realised that the pkd46 plasmid we were trying to transform is heat sensitive, so heat-shocking and incubat 1. RBS BioBricks from the kit were ligated into plasmid, transformed into E. coli DH5-alpha (?), and miniprepped. (this is p

48 KB (7,151 words) - 17:40, 26 October 2013
Team:UANL Mty-Mexico/Results/Protocols
<li>Add plasmid DNA (100 ng) or ligation (up to 5 µL) depending on DNA concentration.</li> <li>Mini preparation of plasmid DNA<ul>

17 KB (2,606 words) - 22:52, 27 September 2013
Team:Greensboro-Austin/MAPs
A two-plasmid system would be appropriate for our aims. One plasmid would code for a library of fp-151 variants, each containing Amber codons i This plasmid would also contain our L-DOPA biosynthesis cassette, ensuring a continual s

15 KB (2,268 words) - 03:47, 28 September 2013
Tim Baker
...ed 16 colonies from Pct 1 and Pct 2 plates to be screened for the BioBrick plasmid *#Using this concentration and that of the given plasmid backbone PSB1C3 set up a gibson assembly

14 KB (2,183 words) - 18:01, 27 September 2013
Team:BYU Provo/Notebook/Cholera - Enzyme/May-June/Period1/Dailylog
...mers for the part that we are getting so that we can use it in the pet-15B plasmid in order to allow us to easily purify the proteins once we get the gene clo ...plasmid to allow us to purify the gene after it is produced, as the pET15b plasmid attaches a His-tag to the protein that causes it to be excreted from the ce

14 KB (2,082 words) - 23:41, 27 September 2013
Team:Colombia Uniandes/Journal
We performed miniprep procedures with the GenElute HP Plasmid Miniprep kit. ...sed primers 6 & 1 (A) and 34 & 9 (B) to extract VP16 and GCR from the Nal1 plasmid.</li>

23 KB (3,678 words) - 02:30, 28 September 2013
Team:DTU-Denmark/Notebook/17 July 2013
=== Plasmid isolation pZA21 with RFP and pZA21 with cycAX from transformants=== According to standard protocol attached to GenElute™ Plasmid Miniprep Kit.

2 KB (339 words) - 20:41, 16 September 2013
Team:Paris Bettencourt/Protocols
...sed to transform chemically competent (i.e. from CaCl2) with a miniprepped plasmid or a ligation product. <h3>Miniprep using Thermo Scientific GeneJET Plasmid Miniprep Kit</h3>

29 KB (4,517 words) - 15:19, 16 October 2013
Team:TU-Eindhoven/StochasticModel
.../Growth%20of%20bacterial%20cultures/ | edition= | pages= | year= }} As the plasmid has a low copy number, we take the average of copy number and multiply it w ... the protein expression rate is linear to the proportion of 'on-state' plasmid.

5 KB (632 words) - 14:23, 26 October 2013
Team:Calgary/Sandbox/Notebook/Journal/Detector
...rt:BBa_K782006" >BBa_K782006</a>) ([A] and [B]) in the RFP generator. This plasmid will be used as a target sequence. Primers were also designed that incorpor ...formed. Subsequently, a overnight cultures of the colonies were made and a plasmid purification was performed. Digestion of the purified plasmids with NotI an

42 KB (7,305 words) - 05:21, 21 September 2013
Team:DTU-Denmark/Notebook/15 August 2013
* Plasmid isolation of CycAX, TAT3-2, TAT3-1a, Sec2, HAO, pZA21::RFP and pZA21::araBA ===Plasmid isolation===

3 KB (386 words) - 11:58, 4 October 2013
Team:Evry/Primers
RBS sfGFP - Plasmid 3 (Forward - GoldenBrick sequence) RBS sfGFP - Plasmid 3 (Reverse - GoldenBrick sequence)

39 KB (3,643 words) - 16:32, 1 October 2013
Team:Evry/Protocols/03
<h1> Plasmid purification </h1> The aim of the plasmid purification step is to recover the plasmid produced by the bacteria (cloning strain). 

3 KB (532 words) - 13:25, 1 October 2013
Team:Goettingen/NoteBook w1
Plasmid mini-prep for Part1-7 Plasmid Mini-Preparation of parts 1 -

75 KB (9,781 words) - 21:23, 30 September 2013
Team:Manchester/Notebooktest2
...d things were really starting to come together. We realised that the pkd46 plasmid we were trying to transform is heat sensitive, so heat-shocking and incubat 1. RBS BioBricks from the kit were ligated into plasmid, transformed into E. coli DH5-alpha (?), and miniprepped. (this is p

46 KB (7,006 words) - 17:22, 24 October 2013
Team:Valencia-CIPF/Notebook
...f DH5-alpha bacteria, while we also ordered the sequencing of the p65NTraR plasmid (BMR Genomics). Moreover we transformed new bacteria in order to amplify a plasmid containing the enzyme beta-glucosidase (bGluc) we received from Dr. C. Fren

15 KB (2,295 words) - 11:03, 11 September 2013
Team:Paris Saclay/Notebook/August/8
... the right size. We will purify the highest band which contains the pSB3K3 plasmid. ... the right size. We will purify the highest band which contains the pSB3K3 plasmid.

5 KB (671 words) - 01:26, 5 October 2013
Team:Dundee/Project/Notebook2
...ng us to ligate our gene construct straight in to the B. subtilis plasmid vector pDR110. ...> finally came to fruition. The construct was cloned in to the appropriate plasmid vector and transformed into B. subtilis cells. On with characteris

7 KB (1,067 words) - 22:08, 1 October 2013
Team:Wisconsin-Madison/protocol
<h3>Transforming the Synthesized Plasmid</h3> ...s for Taq Ligase and T5 exonuclease were designed synthesized in a factory plasmid by GeneArt</li>

13 KB (2,300 words) - 22:56, 19 September 2013
Team:Yale/Project Validate
=== Insertion via Plasmid === ...enes into ''E. coli'', we used Gibson assembly to add our construct onto a plasmid with KanR as a selectable marker.

10 KB (1,295 words) - 00:26, 27 September 2013
Team:Paris Saclay/Notebook/September/28
* RBS_BphR2_part1, BphR2_part2 and plasmid PSB1C3 * FNR_part1, FNR part1 and plasmid PSB1C3

2 KB (269 words) - 05:45, 12 September 2013
Team:INSA Toulouse/contenu/lab practice/notebook/protocols/digest
· Linearized plasmid backbone (The destination plasmid backbone for your composite part) · Linearized plasmid backbone (25ng/µl) 

11 KB (1,693 words) - 09:59, 26 September 2013
Team:Warsaw/Journal
Isolate plasmid DNA by alkaline lysis. Quality cheked with spectrophotomeric analysis with ... with mini-prep kit. Digest and ligation of construct: J23100 and B0034 on plasmid pSB1C3.

3 KB (422 words) - 18:22, 1 October 2013
Team:Heidelberg/Templates/DelH week13
===Generation of DelH Plasmid 26-07=== ===Generation of DelH Plasmid 28-07===

8 KB (1,243 words) - 11:00, 25 October 2013
12/09/13
==Isolate plasmid from overnight culture== ...eI, which are also present in the amplified TOD operon gene and the pSB1C3 plasmid.

5 KB (729 words) - 12:06, 27 September 2013
Team:Biwako Nagahama/Project
We named BBa_K1044006 "pBI107".This plasmid length is about 9,000 bp. DNA synthesis company send us the DNA fragment with pTAKN-2. pTAKN-2 is plasmid that .Thus,We get DNA fragment with pTAKN-2.

20 KB (3,300 words) - 03:36, 28 September 2013
Team:SUSTC-Shenzhen-A/Project
'''pMB1''' and '''p15A''': control plasmid replication in E coli '''pMB1''' and '''p15A''': control plasmid replication in E coli

34 KB (4,851 words) - 03:36, 28 September 2013
Team:ZJU-China/Notebook/LabNotes/July
|Jul 13||Plasmid pBADS purification. 3A assembly of parts pBADS, PhiX174-pE, and pSB1k3. |Jul 17||Plasmid purification of pE-pSB1c3. Transform GFP and 23L, but no results with 23L.

3 KB (546 words) - 09:49, 27 September 2013
Team:USP-Brazil/Protocols
<h4>Plasmid DNA isolation (E.coli)</h4> <h5>Extraction of plasmid DNA from E. coli by Alkaline Lysis – Miniprep</h5>

19 KB (3,066 words) - 02:55, 27 September 2013
Team:Carnegie Mellon/Protocols
Plasmid DNA was purified from 3ml overnight cultures using GeneJET Plasmid Miniprep Kits (Thermo Scientific). ...ned into the pSB1C3 plasmid backbone for BioBrick parts submission, pSB1A2 plasmid backbone containing the WTlac promoter and ligated into the lambda gt11 arm

5 KB (838 words) - 02:03, 28 September 2013
Team:TU-Delft/Protocol 4
<h2 align="center"> Plasmid Purification Protocol</h2> This protocol is based on QIAGEN® Plasmid Purification Handbook. 

4 KB (565 words) - 15:36, 3 October 2013
Team:Tokyo Tech/Project/M13 supplement
<h1>1. M13 plasmid construction</h1> ... of replication origins: M13 origin and pSB origin (p15A). The size of the plasmid is 9130 bp (Fig. 3-8-1).

5 KB (853 words) - 04:18, 27 October 2013
Team:KIT-Kyoto/Notebook/ATF1/september
plasmid DNA was detected. Miniprepped plasmid

43 KB (6,403 words) - 09:19, 27 September 2013
Team:Carnegie Mellon/Week13
Plasmid cultures diluted at 9:30 and IPTG added at 10:45 ... 42ºC heat induction have the same levels of RFP (10x lower than the XL10 plasmid induced for 2 hours at mid-log). 

2 KB (373 words) - 21:35, 26 September 2013
Team:WLC-Milwaukee/Overview
...ll not be suppressed by CymR in the bacterial cells that have taken up the plasmid. toxin, a cymR-regulated T5cumate promoter is placed within the plasmid. This

10 KB (1,611 words) - 00:44, 28 September 2013
Team:Manaus Amazonas-Brazil/fadr
And these plasmid will be transformed in Shewanella This plasmid will be transform in Shewanella putrefaciens

9 KB (977 words) - 02:56, 28 September 2013
Team:Nevada/Notebook/Month1
Miniprep of the 3 liquid cultures and Nanadrop analysis of Plasmid, Liquid cultures were prepared again but with new KM antibiotic. ...endolysin, and ice nuclease target sequence genes in the Genescript (Kan+) plasmid.

3 KB (431 words) - 03:51, 28 September 2013
Team:TMU-Tokyo/Notebook experiment/3rd
<Td>Plasmid name</Td><Td>Fragment 1-A+B</Td><Td>Fragment 2</Td><Td>Fragment 3 - Ap</Td> Transformation following plasmid to Escherichia coli MG1655 red.

7 KB (1,119 words) - 21:49, 27 September 2013
Team:Marburg/Notebook:August
<li>1 µl Plasmid (pSB1C3-iBB39,pSB1A3-iBB49 and pSB1C3-iBB6315)</li> inoculation of colonies for plasmid preparation

7 KB (953 words) - 11:15, 27 October 2013
Team:CAU China/J Aug
Transformation of pET-28a(+) for plasmid propagation Plasmid DNA isolation for pET-28a(+)

5 KB (791 words) - 10:22, 27 September 2013
Team:OUC-China/Results
...d, the figure(on the right) of JM109 strain with both MamC::GFP expression plasmid and artificial gene cluster shows that there are clear MamC::GFP fluorescen

13 KB (1,653 words) - 04:04, 28 September 2013
Team:Uppsala/P-Coumaric-acid-pathway
...ts to use in our assemblies. What promotor should we use? High or low copy plasmid? We wanted to pick parts that we knew would give a high production of our n ...e model after implementing the values in Table 2 is the copy number of the plasmid and the strength of the promoter. 

26 KB (3,985 words) - 17:25, 28 October 2013
Team:Greensboro-Austin/ncAAs
...y recode the Amber codon, the strain RF0 [[#References|[2]]] contains an F plasmid with all the essential genes normally ending in the amber codon mutated to ...emission wavelength of the fluorescence may be shifted. We tested this GFP plasmid in ''E. coli'' cells with the wild-type''M. jannaschii'' tyrosyl-tRNA and s

9 KB (1,280 words) - 03:51, 28 September 2013
Team:NYMU-Taipei/Modeling/Ethanol
#N = number of plasmid in a single cell #N = number of plasmid in a single cell 

10 KB (1,535 words) - 02:39, 29 October 2013
Team:Heidelberg/Templates/Project
...746200) for the outer membrane of E. coli was inserted in another plasmid (pIK1). Team Cambridge revealed in 2007 that Bba_I746200 is toxic. It was i ...promotor as in the pIK8 and a low copy RBS [BBa_B0032]. Another shot was a plasmid without promotor so that E. coli has no need to express and mutate

21 KB (2,691 words) - 16:59, 23 October 2013
Exeter/14 August 2013
| KAN plasmid || 23.6 | KAN plasmid || 23.6 || 10.60 || 5.4

3 KB (497 words) - 20:22, 2 October 2013
Genetic lab journal
Isolate plasmid DNA by alkaline lysis. Quality cheked with spectrophotomeric analysis with ... with mini-prep kit. Digest and ligation of construct: J23100 and B0034 on plasmid pSB1C3.

3 KB (443 words) - 18:31, 1 October 2013
Team:Heidelberg/Templates/DelH week10
===Generation of DelH Plasmid=== ===Generation of DelH Plasmid===

25 KB (3,701 words) - 08:48, 24 October 2013
Team:Heidelberg/AttributionsII
...as a big help in the creation of the synthetic T-domains. Ralf focussed on plasmid and primer design as well as the labwork and was actually considered to be ...Florian Schmidt and Sophie Rabe worked as well on the assembly of the DelH plasmid and the gold precipitation by delftibactin. Sophie Rabe was furthermore res

22 KB (2,846 words) - 22:54, 28 October 2013
Team:Heidelberg/Templates/DelH week20
===Characterization of DelH Plasmid pHM04 30-08 Clones 4, 7, 15=== ...1, 58 were sent in MWG for sequencing. There for we prepared 15 µl of the plasmid (midiprep) with a concentration of 50-100 ng/µl and add 2 µl DN07 primer

23 KB (3,522 words) - 21:22, 25 October 2013
Team:Heidelberg/Templates/M-15-05-13
* will be integrated into plasmid for electroporation in ''E. coli'' * Lambda-red plasmid

4 KB (535 words) - 00:30, 4 October 2013
Team:Frankfurt/Notebook/Labwork
3. Digest of plasmid p426 (mevalonate) with and without insert. 6. Overnight cultures of yeast with the mevalonate plasmid. 

2 KB (319 words) - 23:42, 4 October 2013
Team:Heidelberg/Tyrocidine week16 ms
...ide I: fragment 12 was given in excess, as it was missing in the sequenced plasmid ...ext day lead to a positive result (figure XY). In this case, the validated plasmid that was used for transformation was used as a positive control.

7 KB (958 words) - 16:28, 4 October 2013
Team:ITU MOBGAM Turkey/protocol
...;1. Put 1μL of circular plasmid or all of a ligation reaction of plasmid DNA in a microtube. Gently add ~100μL of competent cells. Do NO DNA contro ...rong>   2. Digest your two parts and construction plasmid backbonedestination vector with the following enzymes

15 KB (2,323 words) - 22:49, 4 October 2013
Team:SJTU-BioX-Shanghai/Results/Test/Overall
==Plasmid mRFP test== Unlike plasmid genes, genome-residing genes are generally single-copied, thus may behave d

7 KB (1,003 words) - 01:38, 17 January 2014
Team:Penn/AssayValidation
...nverted DNA. Primer Set 2 was successful and is included with our MaGellin plasmid, much like VF and VR are included as standardized biobrick sequencing prime ...ation (Figure 3). These experiments helped us optimize the ideal amount of plasmid and restriction enzyme to use in any study moving forward.

5 KB (778 words) - 02:53, 29 October 2013
Team:Tianjin/Project/Alk-Selector&DirectEvolution
...gether and then whole plasmids were extracted from it to get the amplified plasmid library of alkane producing module. This library was later transformed into ...d the requirement. At the same time, the strains that didn’t take in the plasmid would eliminate under the pressure of antibiotic in this process. Then, all

20 KB (2,755 words) - 03:07, 29 October 2013
Team:TU-Munich/Team/Attributions
Beside this plasmid, we also obtained a plasmid for the TEV protease from the chair which we have used to generate the Spli ''They kindly provided us with the pSH21 plasmid which was used as template for the Polioviral Internal Ribosome Entry Site

18 KB (2,659 words) - 02:43, 29 October 2013
Team:Braunschweig/Protocols
<h2><a href="#Miniprep">Minipreparation of Plasmid DNA</a></h2> Miniprep was done with Plasmid Miniprep Kit I by Peqlab (High Copy) following the manufacturer’s protoco

22 KB (3,259 words) - 13:16, 27 October 2013
Team:MSOE Milwaukee/Week3
...e expression. We also decided to use three genes or two biobrick parts per plasmid to optimize expression and ensure that the plasmids do not reach capacity. *Plasmid 1: HMG-CoA synthase, HMG-CoA reductase, and Mevalonate kinase 

3 KB (473 words) - 13:40, 22 August 2013
Team:UCL/Notebook
The Biobricks from the iGEM HQ arrived today, which includes a mammalian plasmid backbone and 2 auxin signalling parts. ...xipreping the recombinant zeocin plamid as well as on the MMP9 recombinant plasmid.

44 KB (7,423 words) - 03:44, 5 October 2013
Team:CSU Fort Collins/Beer
...ing plasmids, but we were also in the process of developing an integrative plasmid that would insert our sequence directly into the yeast genome. <li>pCM189: centromeric yeast plasmid, marker URA3, tetracycline repressed expression of target gene under contro

6 KB (1,009 words) - 04:03, 28 September 2013
Team:WLC-Milwaukee/Notebook
plasmid extraction and glycerol stock creation. After plasmid extraction the and plasmid isolation. 

7 KB (1,229 words) - 03:48, 28 September 2013
Team:Warsaw/Protocols
# Add a cooled plasmid or ligation in a volume not bigger than 20 μl and stir with a tip # 50 μl of bacteria pipete to a dialised and cooled plasmid DNA or ligation

10 KB (1,652 words) - 02:01, 5 October 2013
Team:Baskent Meds/Notebook
plasmid isolation (P1 18A) ...ots of ligations and restrictions. Transformations went pretty well, so as plasmid isolations.

18 KB (2,992 words) - 18:26, 4 October 2013
Team:Glendale CC AZ/Protocols/AlkalineLysis
.../2013.igem.org/Team:Glendale_CC_AZ/Protocols/AlkalineLysis">Alkaline Lysis Plasmid Miniprep </a> ==== Alkaline Lysis Plasmid Miniprep Protocol ====

11 KB (1,290 words) - 02:35, 28 September 2013
Exeter/1 August 2013
D - AMP plasmid (E+P+D) - 40.4 Instead of using an RFP plasmid from a transformation/mini-prep, we're using one resuspended from a kit pla

3 KB (517 words) - 19:35, 2 October 2013
Team:BYU Provo/Notebook/CholeraDetection/SummerExp/Period4/Dailylog
...e on hand in frozen stock. Froze down pIG12+Cro(PstI/EcoRI) as pIG87. This plasmid has been sequence verified. ...lace on ice. Pipet 50 uL of cells into a microcentrifuge tube with 5 uL of plasmid. Pipet the solution into a silver-plated cuvette that has been sitting on i

6 KB (896 words) - 05:55, 23 September 2013
09/08/13
==Isolating plasmid== *Isolated the plasmid using omega bio-tek Plasmid mini kit I

3 KB (372 words) - 15:12, 12 September 2013
Team:Bordeaux/Project
...ce betanine from leucodopachrome. The strategy of the team was to insert a plasmid with one of these two enzymes in Lactobacillus, to make it produce betanine Plasmid with the cyclo-DOPA 5-0-glucosyltransferase gene:

17 KB (2,623 words) - 03:51, 5 October 2013
Team:Ciencias-UNAM/Project/WetLab/Characterization
... is not transcribing any LL-37 that could be making the cells to loose the plasmid. The second problem we addressed was the change in the codon usage they use ... plasmid (we made pressure selection with chloramphenicol to make sure our plasmid was still there). We did the inoculation at 0.05 of OD at 600A and incubati

18 KB (2,723 words) - 04:01, 28 September 2013
Team:NTU-Taida/Notebook/Protocol
#Your two part samples: Miniprepped DNA (in BioBrick RFC[10] plasmid backbones) #Linearized plasmid backbone (with a different resistance to the plasmid backbones containing your part samples)

7 KB (1,062 words) - 03:40, 28 September 2013
Team:CU-Boulder/Project/Kit/DNAPurification
... making our own miniprep spin columns that is much cheaper than commercial plasmid purification kits. Additionally, we tested and validated an effective metho ...cing colonies and ~50% RFP producing colonies resulting from contaminating plasmid from the previous purification. Recycling works!

2 KB (342 words) - 17:01, 27 October 2013
Team:KU Leuven/Project/Glucosemodel/EBF
... used for the biosynthesis of lots and lots of compounds. Once we insert a plasmid containing the β-farnesene synthase gene, we may obtain only a very small ...>ERG8, MVD1, idi and ispA genes into a pBBR1MCS-3 plasmid. Coexpression of these two operons in an ispC deficient E. coli</

34 KB (5,339 words) - 03:06, 29 October 2013
Team:UNITN-Trento/Notebook/Labposts/07/19
...SE (µl)</th> <td>1 </td> <td>1 </td> <td>1</td> <td>1</td> </tr> <tr> <th>PLASMID (µl)</th> <td>2.5</td> <td>2.5</td> <td>2.5</td> <td>2.5</td> </tr> <tr> <

2 KB (384 words) - 10:36, 3 October 2013
Team:MIT/MaterialsAndMethods
<LI> Transfer 1-5mL of overnight culture of plasmid cells into 2ml microcentrifuge collection tubes (1 per try) provided in the <LI> Transfer the overnight culture of plasmid cells into a 50 mL tube.</LI>

41 KB (6,854 words) - 03:20, 29 October 2013
Team:Uppsala/affinity-tags
...to use antibiotics and antibiotic resistance. If a clone were to lose the plasmid, the toxin which usually has a longer half life than the antitoxin will kil <img class="method-plasmid" src="https://static.igem.org/mediawiki/2013/d/dc/Uppsala2013_anti-toxin-to

8 KB (1,147 words) - 05:42, 29 September 2013
Team:TzuChiU Formosa/Project
...hing wrong with the competent cell and in fact we have obtained a complete plasmid from digestion. Therefore, we assume the reasons could be the following: b.     The size of the plasmid is too large

22 KB (3,145 words) - 01:56, 28 September 2013
9 August 2013
==Isolating plasmid== *Isolated the plasmid using omega bio-tek Plasmid mini kit I

3 KB (367 words) - 12:11, 2 September 2013
Team:UANL Mty-Mexico/Results
...lones transformed with the same DNA (figure 4). We suspected variations in plasmid copy number (PCN) to be the potential cause of phenotypic discrepancies amo ... width=400px><figcaption>Figure 5. Plasmid DNA concentration by clones (ng/uL). </figcaption>

6 KB (970 words) - 05:29, 28 October 2013
Team:Northwestern/methods
...e implemented as intended. We used a low-copy plasmid, because a high-copy plasmid would put too much stress on our cells and hinder their survival.

7 KB (966 words) - 04:04, 28 September 2013
Team:Peking/Project/BioSensors/NahR
...ters to activate transcription in response to the inducer salicylate. This plasmid encodes enzymes for the metabolism of naphthalene or salicylate as the sole ... cluster in Pseudomonas putida. (a) Gene cluster on the NAH7 plasmid that degrades naphthalene: Naphthalene is degraded into salicylate by enzym

31 KB (4,208 words) - 18:15, 28 October 2013
Team:Chiba/Project/uptake
... To best enjoy the portability of plasmid (BioBrick), it is ideal to make plasmid-coded gene-knockout system. To this end, we decided to establish temporal ...ng down each of fur, fieF, gor, and trxB. Alongside, we also constructed a plasmid knocking down lacZ. 

9 KB (1,470 words) - 04:16, 28 September 2013
Team:Carnegie Mellon/Project/Procedure
 <h3>Plasmid Construct</h3> ...level expression and to prove that KillerRed is able to kill bacteria. The plasmid construct consisted of a wild type lac promoter, like the phage and

5 KB (741 words) - 02:14, 28 September 2013
Team:BYU Provo/Notebook/CholeraDetection/Fallexp/Period1/Dailylog
...f) from here on out: they include cloning our parts into the iGEM backbone plasmid, filming a synthetic biology techniques video to collaborate with another t ...t grew following an electroporation procedure to get TT9907 to take up the plasmid pIG87. Amazingly, top agar lawn assays with a drop of hydrogen peroxide ad

10 KB (1,705 words) - 06:38, 26 September 2013
Team:Goettingen/NoteBook w8
.../span>Minipreps performed with the Plasmid purification kit, elution in 30µl pre-warmed water, AW buffer step include ...p class="c8-0 c8-18">RBS-DarR ORF into part7(Term plasmid)    &nb

32 KB (5,760 words) - 21:19, 30 September 2013
Team:INSA Toulouse/contenu/lab practice/notebook/calendar/logic gates
Insertion of And 1 gate extracted by PCR into the plasmid backbone pSB1K3 ...n worked well, but on the electrophoresis profile we got only the backbone plasmid with no insert. 

14 KB (2,056 words) - 02:19, 5 October 2013
Team:Hong Kong HKUST/notebook/mod2
· Plasmid extraction of pBlueScript KS(+) · Inoculation of pEGFP-N1 and BBa_K817002 (PfadBA) for plasmid extraction 

37 KB (5,673 words) - 23:14, 27 September 2013
Team:UCSF/Project/Conjugation/Design
...ary for conjugation are contained in the genome) and carries a conjugative plasmid, which codes for a catalytically dead Cas9 (dCas9) protein and guide RNA (g Upon conjugation with the target population, the conjugative plasmid would be transferred. Both dCas9 and gRNA would subsequently be expressed i

5 KB (640 words) - 18:53, 23 October 2013
Team:UCSF/Project/Circuit/Design
...gRNA. The gRNA will then form a complex with dCAS9, which is on a separate plasmid, and block the production of the opposite fluorescent protein. Only one flu ...NA will then combine with dCas9, which has been incorporated on a separate plasmid. The dCas9-gRNA complex will then repress the RFP (left panel). In scenari

9 KB (1,307 words) - 00:11, 28 October 2013
Team:Virginia/Results
...s to determine the relative growth rate of the XL1-Blue cells that all the plasmid constructs were tested in. The growth curve and its associated data can be ...r the expression of GFP and overexpression of GFP, XL1-Blue cells with the plasmid construct were grown up in LB media with a wide array of IPTG concentration

20 KB (2,924 words) - 16:36, 22 November 2013
Team:Cornell/project/wetlab/fungal toolkit/biosafety
...aterial between organisms is most prevalent in prokaryotic organisms where plasmid DNA can be easily expelled and absorbed. When working with eukaryotes like ...ism, so if no selective pressure is used, then the organisms can expel the plasmid [3]. We hypothesize, however, that the lack of selective pressure will not

8 KB (1,221 words) - 02:32, 29 October 2013
Team:Tokyo Tech/Experiment/Crosstalk Circumvention Assay
...lux/tet hybrid promoter, GFP and lasR on the reporter plasmid (Fig. 3-2-2). .... We used Pcon-RBS-luxR-TT-Ptrc-RBS-tetR-TT as the regulator plasmid.

10 KB (1,635 words) - 03:05, 29 October 2013
Team:Tokyo Tech/Experiment/pSB-M13 Plasmid Assay
pSB-M13 Plasmid Assay ...Tech/Project/M13_supplement#1._M13_plasmid_construction See more about our plasmid construction]).

4 KB (644 words) - 17:14, 28 October 2013
Team:BIOSINT Mexico/Chassis
...bacteria . When pUB110 replicates in cells of B.subtilis or S. aureus, the plasmid regions, rep, ori, and BA3, function as a structural gene that encodes a re ...e plasmid in its active host (7, 33), effects of palA and the IG region on plasmid stability were examined in L. casei MSK248.. These results indicate that pa

4 KB (577 words) - 04:17, 28 September 2013
Team:HIT-Harbin/Experiments
Part A :Plasmid carrying Plac+RBS+hrpR+T Part B :Plasmid carrying Ptet+RBS+hrpS+T

7 KB (1,000 words) - 14:26, 27 September 2013
Team:Tsinghua/Project-Reporter
At first, we design a test plasmid to determine the feasibility of applying Tet-off system in our system. Afte ...e of deprivation of adenine, while the wild type yeast is in white. If the plasmid is transformed into ADE2 knock out yeast strain, TetR domain of tTA will bi

8 KB (1,127 words) - 20:03, 27 September 2013
Team:LZU-China/Notebook
Amplified IκB fragment from the Plasmid of by Polymerase Chain Reaction(PCR). Amplified GFP fragment from the Plasmid of pEGFP-N1 by Polymerase Chain Reaction(PCR).

21 KB (3,175 words) - 03:57, 28 September 2013
Team:Tsinghua/Project-Switching-System
...tR-VP16 was under the control of cyc100 mini promoter. We constructed pTF5 plasmid. ...ragment from TRE to CYC1 terminator was inserted in to pRS415. We got pTF7 plasmid.

6 KB (893 words) - 20:05, 27 September 2013
Team:SYSU-Software/designer
Plasmid: ...omation is our soul, the plasmid is constructed without your worrying. The plasmid will be presented intuitively and detailedly. Even better, you can select d

10 KB (1,360 words) - 23:19, 28 October 2013
Team:KIT-Kyoto/Notebook/ATF1/august
Miniprepped plasmid Digested plasmid

75 KB (10,859 words) - 09:18, 27 September 2013
SCU Weekly
*4.plasmid extraction of F2622 + BBa_CO261 *3.extracted plasmid of K2-8B, F2622 + BBa_CO261 and only K2-8B was successful

10 KB (1,583 words) - 17:40, 1 October 2013
Team:UC Chile/Lab NoteBook
...ted working on our Carbo project by making our own stock of this important plasmid. Also, we took some of the biobricks from the 2012 distribution kit necessa ...li>E. coli transformation with pHnCBS1D (carboxisome’s plasmid).</li>

69 KB (7,400 words) - 23:11, 26 September 2013
Team:UC Chile/Game
...earch, you need to gather the pieces of DNA that will form the recombinant plasmid, and all this while you are learning about synthetic biology. The final structure of the plasmid is: 

29 KB (4,334 words) - 00:29, 8 October 2013
Team:UC-Santa Cruz/Notebook1
...recombinant plasmids were then transformed into competent e.coli cells for plasmid amplification. The amplified plasmids were isolated and Purpose: colony plasmid isolation

71 KB (11,188 words) - 04:00, 28 September 2013
Team:ZJU-China/Notebook/LabNotes/August
|Aug 2||Link GFP with S+P. PCR amplification of pE-DTer. Small-scale plasmid purification of LuxR and LuxT. ...B1A3 with Xba I and Spe I and then do the gel electrophoresis. Small-scale plasmid purification with FA.

4 KB (594 words) - 22:38, 27 September 2013
Team:Duke/Notebook/Protocols
#Plasmid-Safe nuclease treatment: To each of your 1st cycle reactions add: #*1ul Plasmid-Safe nuclease

12 KB (1,834 words) - 06:38, 27 September 2013
Team:NYMU-Taipei/Modeling/ModSensors
N = number of plasmid in a single cell N = number of plasmid in a single cell

6 KB (976 words) - 02:23, 29 October 2013
Team:Toronto/Notebook
deletion strains with a pKD46 plasmid, recombinases, and PCR. Researched plasmid region for our master plasmid was constructed through PCR extension of two

30 KB (4,922 words) - 03:11, 28 September 2013
Team:Penn/Biobricks
<h4>MaGellin Plasmid Backbone <a href="http://parts.igem.org/Part:BBa_K1128001">(BBa_K1128001)</ ...ntify and quantify the location and amount of CpG DNA methylation. The one-plasmid design ensures simple customization of the DNA-binding domain and targeting

9 KB (1,380 words) - 02:54, 29 October 2013
Team:Freiburg/Highlights
...ased gene regulation experiment. With our toolkit and the standardized RNA-plasmid, termed <a id="link" href="https://2013.igem.org/Team:Freiburg/Project/crrn ... For multiple targeting, different crRNAs can be combined into one RNAimer plasmid. Gene regulation worked even more efficiently when using multiple targets.

31 KB (4,080 words) - 14:32, 25 November 2013
Team:Hong Kong CUHK/protocol
<a href="#a1.9">1.9 Plasmid DNA Extraction (Using TaKaRa MiniBEST Plasmid Purification Kit Ver.4.0)</a> 2. Mix loading dye and the insert/plasmid before adding to the wells. For example, if the DNA we have got is 45μl,

28 KB (4,059 words) - 10:15, 27 October 2013
Team:Hong Kong CUHK/june
...of pSB1K3-RFP plasmid. Total 120ml obtained. Ran gel and confirmed correct plasmid obtained. Found that at least 20 ml TAE is needed. 4. Run gel to confirm correct plasmid obtained. ( T7+RBS is small, so band should appear at very end of the gel,

10 KB (1,547 words) - 10:32, 27 October 2013
Team:Grenoble-EMSE-LSU/Project/Biology/KR
...t antibiotics (chloramphenicol, ampicilline and streptomycin, one for each plasmid), with lag phases exceeding 24 hours. ...soning was that faster LB growth might allow cell culture without too much plasmid loss. Like expected our cells grew quickly and reached the stationary phase

17 KB (2,670 words) - 02:07, 5 October 2013
Team:Uppsala/results
...gative control is an extract from a strain with no TAL gene on transformed plasmid. The positive control is an extract a culture of the same strain as the neg ...gative control is an extract from a strain with no TAL gene on transformed plasmid. The positive control is an extract a culture of the same strain as the neg

28 KB (4,034 words) - 17:10, 28 October 2013
Team:Goettingen/NoteBook w-2
"Arial","sans-serif"">Plasmid prep of pGP172 and digestion with SacI and ...le="font-size:11.0pt;font-family:"Arial","sans-serif"">Plasmid

46 KB (5,991 words) - 20:33, 30 September 2013
Team:NTNU-Trondheim/Press/examplePage
The expression plasmid consists of a stress promoter regulating the production of ToxR. This const ...mg/ml, 1.5 mg/ml, and 0 mg/ml arabinose. For our control, a known nontoxic plasmid was transformed into the cells that should not activate our reporter strain

11 KB (1,730 words) - 21:22, 30 September 2013
Team:Heidelberg/Templates/DelH week18
===Generation of DelH Plasmid pHM05 26-08=== ===Generation of DelH Plasmid pHM04 and pHM05 30-08===

27 KB (3,718 words) - 16:46, 25 October 2013
(to learn more about our sensor model, click here)
N = number of plasmid in a single cell N = number of plasmid in a single cell

7 KB (1,051 words) - 02:51, 28 October 2013
Team:UCL/Labbook/Week11
... media in two falcon tubes each (1x drug, 1x no drug), two plates for each plasmid were also streaked (1x drug, 1x no drug). All were left to incubate at 37° <th>Plasmid Insertion</th>

16 KB (2,806 words) - 23:53, 4 October 2013
Team:Gdansk-UG/Project
...imers matching our promoter sequence and ligation of the PCR product with plasmid Backbone.  ..., although it’s hard to transform, would be a best host to our plasmid due to its high resistance to ethanol.  

10 KB (1,218 words) - 16:28, 3 October 2013
Team:Heidelberg/Templates/MM week15p
* no PCR product, miniPrep runs above 10 kb supercoiled (whole plasmid is expected to have 9 kb) (miniPrep has ca. 10 ng/µl) * need to verify plasmid identity: linearize with BamHI (unique restriction site in both pET21c... a

5 KB (663 words) - 03:38, 5 October 2013
Team:Tuebingen/Notebook/Protocols/3a-assembly
<td>Plasmid DNA</td> ... with EcoRI / SpeI, downstream part with XbaI / PstI, and destination part plasmid with EcoRI / PstI.</li>

3 KB (433 words) - 12:11, 4 October 2013
Team:Heidelberg/Templates/Del week10 overview
...|thumb|Vector map of [[:File:Heidelberg_Psb4k5+BBa J04450 DelRest.gb| pFSN plasmid]] including Primers FS_01 to FS_16 used for assembly of the desired genes f ...' in ''E. coli'' for efficient production of delftibactin, we designed the plasmid pFSN. It comprises the above listed genes as inserts. Furthermore, it inclu

8 KB (1,126 words) - 01:28, 29 October 2013
Team:Frankfurt/Project/Results
...gap repair was done this year. We were able to grow cultures containig the plasmid on selective media with the corresponding auxotrphy, but unfortunately we w Since the first plasmid created last year leads to a higher amount of geranylgeranylpyrophosphate,

1 KB (191 words) - 01:43, 5 October 2013
Team:BYU Provo/Notebook/Cholera - Enzyme/October/Period1/Dailylog
We ran the plasmid prep kit on the pelleted PIG92 overnight. The purified plasmid was stored in the freezer in our parts registry. ...in the fridge until we can verify if any of the eight colonies contain our plasmid.

4 KB (671 words) - 21:23, 16 October 2013
Team:TU Darmstadt/protocols/Miniprep
 <h2> PureYield Plasmid Miniprep System </h2>

6 KB (738 words) - 00:37, 5 October 2013
Team:UCSF/Project/Conjugation/Design1
...ary for conjugation are contained in the genome) and carries a conjugative plasmid, which codes for a catalytically dead Cas9 (dCas9) protein and guide RNA (g <p2> Upon conjugation with the target population, the conjugative plasmid would be transferred. Both dCas9 and gRNA would subsequently be expressed i

16 KB (1,813 words) - 03:32, 29 October 2013
Team:METU Turkey/protocols.html
...-50">hour recovery time helps in transformation efficiency, especially for plasmid backbones with plasmid backbone, and antibiotic resistance. Plate 20 µl and 200 µl of the transf

34 KB (5,471 words) - 03:38, 5 October 2013
Team:Heidelberg/Templates/Indigoidine week9
We want to assemble pKH1, which is a pSB1C3-derived plasmid containing pSB1C3-lacPromotor-BBa_B0034-bpsA(pMM64)- * prepare primer for bpsA only plasmid for BAP1 validation

5 KB (706 words) - 15:35, 21 October 2013
Team:UCSF/Project/Circuit/Design1
...gRNA. The gRNA will then form a complex with dCAS9, which is on a separate plasmid, and block the production of the opposite fluorescent protein. Only one flu ...NA will then combine with dCas9, which has been incorporated on a separate plasmid. The dCas9-gRNA complex will then repress the RFP (left panel). In scenari

20 KB (2,525 words) - 03:55, 29 October 2013
Team:TecMonterrey/010
...ed for the GFP expression. This expression cassete was cloned into a pUC57 plasmid. ...liberated into the environment and some way it survived and maintained its plasmid without a selectivity marker, it would be a catastrophe. This imaginary sce

53 KB (7,791 words) - 23:11, 28 October 2013
Team:TecMonterrey/011
...ed for the GFP expression. This expression cassete was cloned into a pUC57 plasmid. ...liberated into the environment and some way it survived and maintained its plasmid without a selectivity marker, it would be a catastrophe. This imaginary sce

54 KB (7,838 words) - 23:33, 28 October 2013
Team:TecMonterrey/012
...ed for the GFP expression. This expression cassete was cloned into a pUC57 plasmid. ...liberated into the environment and some way it survived and maintained its plasmid without a selectivity marker, it would be a catastrophe. This imaginary sce

54 KB (7,840 words) - 23:48, 28 October 2013
Team:TecMonterrey/Project.html
...ed for the GFP expression. This expression cassete was cloned into a pUC57 plasmid. ...liberated into the environment and some way it survived and maintained its plasmid without a selectivity marker, it would be a catastrophe. This imaginary sce

54 KB (7,870 words) - 03:55, 29 October 2013
Team:Utah State/Safety
...re on a plasmid backbone with antibiotic resistance, without selection the plasmid would not be maintained in E. coli. ...i were to be released into the environment they would not maintain the plasmid during replication as there would not be any antibiotic in the environment.

14 KB (1,744 words) - 04:03, 28 September 2013
Team:SJTU-BioX-Shanghai/Project/Light sensor/Blue
...ge, we find an interesting published paper named "From Dusk Till Dawn: One-Plasmid Systems for Light-Regulated Gene Expression", which also provides us with l The plasmid we get at last is as follows:

6 KB (954 words) - 03:06, 29 October 2013
Team:Virginia/Attributions
...<div id="pictext"><h2>Virginia Miller, PhD, and Kimberly Walker, PhD - Ail Plasmid</h2> ...Dr. Walker, a research professor in the Miller lab, kindly donated the ail plasmid used by our applications team, while also providing invaluable expertise r

17 KB (2,288 words) - 02:54, 7 November 2013
Team:British Columbia/Modeling
...ble for the production of either cinnamaldehyde or vanillin and the CRISPR plasmid contains unique spacer elements, granting both strains immunity against "en

16 KB (2,633 words) - 12:49, 7 August 2014
Team:Greensboro-Austin/Team
... advanced techniques were demonstrated, such as advanced primer design for plasmid assembly, and the use of non-canonical amino acids for protein engineering. ...Designing fp-151, MAPs cloning, biobricked tRNA-synthetase pair, built OSI plasmid, GFP fluorescence with different synthetases.

8 KB (1,082 words) - 03:45, 28 September 2013
Team:Newcastle/Project/plants
...istance. A transformation vector would have been used rather than a normal plasmid so that ''gusA'' would integrate through homologous recombination into the

18 KB (2,720 words) - 18:43, 28 October 2013
Team:Goettingen/Team/Reporter
...nerator [http://parts.igem.org/Part:BBa_E0240 BBa_E0240]. In the very same plasmid but oriented in the opposite direction, we assembled a darR expressi ...g/Part:BBa_K1045017 BBa_K1045017]) (Fig. 3, right image) or with a control plasmid containing the GFP expression unit only ([http://parts.igem.org/Part:BBa_K1

16 KB (2,360 words) - 09:30, 28 October 2013
Team:NTNU-Trondheim/Protocols
* Linearized plasmid backbone (with a different resistance to the plasmid backbones containing your part samples) ** Digest linearized plasmid backbone with EcoRI and PstI (0.5 ul of each) 

20 KB (3,098 words) - 20:58, 4 October 2013
Team:UTK-Knoxville/methods
..."ltr">Plasmid Extraction: ...nternal-guid-3c286855-68a8-2624-ed13-2832044f6359" dir="ltr">Add 400 uL of Plasmid wash buffer.  Centrifuge 30 seconds.</li>

40 KB (5,705 words) - 16:25, 10 July 2013
Exeter/8 July 2013
==MiniPrep plasmid extraction== ... we use for the rest of the MiniPrep. This is to get the maximum amount of plasmid as possible from the transformed cells.

3 KB (563 words) - 20:38, 1 October 2013
Team:DTU-Denmark/Notebook/26 June 2013
** Tube 1-6 containing 5uL pZA21 plasmid template and 3uL of each primers for amplification of the backbone. ** Tube 15-18; 5uL GFP SF plasmid template and 3uL of each primers for GFP SF TAT.

4 KB (618 words) - 21:04, 16 September 2013
Team:DTU-Denmark/Methods
==Plasmid isolation== [[Team:DTU-Denmark/Methods/Plasmid_isolation|Plasmid isolation by ethanol precipitation]]

2 KB (246 words) - 12:31, 1 October 2013
Team:Evry/Notebook/w4
<li> Plasmid 1K3 <li> Plasmid 1C3

7 KB (995 words) - 10:46, 7 September 2013
Team:UCSF/Project/Attribute
...the pCOLA plasmid as well as inserting the XylE and LacZ gene in the pCOLA plasmid. Ian also worked on the primers for the GFP cassette for the conjugation pr <li>Derrick Lee worked on the creation of plasmid A for the synthetic circuit by inserting the GFP & RFP and the gRNAs for bo

6 KB (944 words) - 03:22, 28 September 2013
Team:DTU-Denmark/Notebook/12 July 2013
===Plasmid isolation of biobricks from transformants made on [[Team:DTU-Denmark/Notebo Isolation was performed according to protocol given in the kit PowerPrep HP Plasmid Miniprep Kit

4 KB (639 words) - 11:38, 4 October 2013
Team:Evry/Notebook/w6
...ither bacteria transformed by empty PSB1A3 plasmid or 1 uL of the miniprep plasmid. This was realised in order to verify if the problem of our first PCR was d ...FUR BS constructions. The positiv control was prepared by using the PSB1A3 plasmid. The same PCR program was used but we were out of One Taq so we utilize Dre

4 KB (688 words) - 07:52, 26 August 2013
Team:Evry/Notebook/w8
<h2>Plasmid 3:</h2> ...second construction, 2 for the third construction and one for the controle plasmid.

2 KB (372 words) - 07:53, 26 August 2013
Team:Evry/Notebook/w12
We did not have anymore AceB with GFP plasmid available to realize the transformation. We pre-cultured a remaining glycer ...the pre-culture of the 03/09/13. As a consequence, we transformed our last plasmid in a TOP10 strain.

4 KB (604 words) - 14:46, 9 September 2013
Team:Grenoble-EMSE-LSU/Documentation/Notebook/July
- Do a miniprep but since the Voigt’s plasmid are low copy, I use 4 Eppendorf for 1 columns to get more concentrated DNA. In the end, there are more than 20ng/µL for all the plasmid. For others colonies, there are more than 100ng/µL - it may be contaminate

15 KB (2,331 words) - 03:36, 5 October 2013
Team:Grenoble-EMSE-LSU/Documentation/Notebook/August
...estriction enzyme used - EcoRI - seems to have a star-activity and cut the plasmid everywhere. ...em comes from the plasmid since the buffers and the RE work on and another plasmid.

8 KB (1,093 words) - 01:28, 5 October 2013
Team:DTU-Denmark/Notebook/24 July 2013
* plasmid pZA21 and AMO * plasmid pZA21 and HAO

4 KB (574 words) - 22:04, 29 September 2013
Team:SYSU-China/Notebookt/Methods
...ion endonuclease, gel extraction, ligation, transformation, colony PCR and plasmid extraction is implemented. Besides, for quantitive measurement, qPCR and we ...n mix or digestion mix), put on ice for 3-5min. This step is optional when plasmid is transformed.

17 KB (2,896 words) - 01:29, 29 October 2013
Team:Wageningen UR/Notebook
...restriction sites for cloning our GFP+Actin construct into the recommended plasmid for parts registry. With these primers, actin-GFP constructs were amplifie Using GeneJET Plasmid Miniprep Kit, we extracted the plasmids containing chromoprotein genes from

52 KB (7,017 words) - 01:16, 5 October 2013
Team:DTU-Denmark/Notebook/6 August 2013
* Plasmid isolation: HAO in pZA21 from USER cloning. ===Plasmid miniprep===

4 KB (548 words) - 22:09, 29 September 2013
Team:Paris Bettencourt/Notebook/TB-ception/Monday 29th July.html
...t we still don't really know that because we need the full sequence of the plasmid. <img src="https://static.igem.org/mediawiki/2013/a/a9/Plasmid.png" />

2 KB (358 words) - 10:20, 9 August 2013
12/07/13
==Transformation of RFP control plasmid== ***Negative control (No plasmid)

1 KB (176 words) - 10:23, 14 August 2013
Team:Manaus Amazonas-Brazil/fadd
purified again and ligate this with a plasmid (PBS1C3) digested contrusction are insert in PSB3C5 plasmid), to compare the

8 KB (965 words) - 03:01, 28 September 2013
Team:UC Davis/Notebook/Week 7
...h the newly mutated <a href="http://parts.igem.org/Part:pSB3K3">pSB3K3</a> plasmid. We also tried ethanol precipitating some DNA in order to prepare it for el ...with our new strain DH10B. Tomorrow we will do a miniprep and sequence the plasmid for the miniprep. These will be cultured overnight. Unfortunately, all of t

6 KB (1,035 words) - 22:33, 27 September 2013
Team:Paris Saclay/Notebook/July/1
===='''1 - Digestion of pSB1C3 plasmid and PCR products : Pndh* by EcoRI/ PstI'''==== ** Plasmid : 4µL

2 KB (277 words) - 00:48, 5 October 2013
Team:Calgary/Project/OurSensor/Detector
Since, our system uses two TALEs we made a plasmid containing both TALE A and B target site annotated [A] and [B] respectively ...re then washed and soaked in a solution containing [A] and [B] on the same plasmid(<a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1189006" >BBa

34 KB (5,857 words) - 03:59, 29 October 2013
Exeter/12 August 2013
| AMP plasmid || 60.0 || 4.17 | AMP plasmid || 4.17 || 11.83

2 KB (210 words) - 20:40, 2 October 2013
Team:Exeter/Results
...to the cyan pigment coding region, and are now working on transforming the plasmid into EnvZ deficient cells (kindly provided to us by the [https://2013.igem. .... Hence, we will express the Red Light Module in EnvZ deficient cells. The plasmid for the Red Light Module contains coding for EnvZ, but also for the second

25 KB (4,115 words) - 03:41, 5 October 2013
Team:UGent/CloneManager
<h1> Plasmid containing construct for chromosomal evolution</h1> This plasmid contains the ccdA antitoxin and the reporter gene gfp. The ho

2 KB (313 words) - 01:50, 5 October 2013
Team:Freiburg/Notebook/crrna
...ef="https://2013.igem.org/Team:Freiburg/Notebook/lab_crrna_plasmid"> crRNA-Plasmid </a> ...tps://2013.igem.org/Team:Freiburg/Notebook/lab_gfp_reporter"> GFP-reporter-plasmid </a> 

3 KB (328 words) - 05:08, 28 October 2013
Week5
...mpts, we have eventually decided to use the traditional way to extract our plasmid for greater efficiency. </td> ...l-align"top">Conquering our ups and downs, our team has finally moved onto Plasmid extraction today. 

15 KB (2,015 words) - 00:04, 28 September 2013
Team:Paris Bettencourt/Notebook/Phage Sensor/Tuesday 13th August.html
<TR><TD>Template Plasmid</TD><TD>0.25µL</TD></TR> for Backbone (M13mp18 plasmid): 3ug 

3 KB (603 words) - 13:37, 7 September 2013
Team:Paris Bettencourt/Notebook/Phage Sensor/Saturday 13th July.html
<TR><TD>Template Plasmid</TD><TD>0.25 ul</TD></TR> for Backbone (M13mp18 plasmid): 3ug 

3 KB (541 words) - 15:21, 23 August 2013
Team:Paris Bettencourt/Notebook/Phage Sensor/Monday 19th August.html
...ion (RFP into M13mp18 backbone), Trafo of Ligation product (RFP in M13mp18 plasmid) into NEB turbo and Test Xgal <TR><TD>Template Plasmid</TD><TD>0.25µL</TD></TR>

4 KB (659 words) - 17:51, 23 August 2013
Team:ETH Zurich/Materials
*Sigma Aldrich Miniprep kit : Elute in 50μl for higher plasmid concentrations. *2ug of plasmid (max 45μl for low concentration minipreps) 

11 KB (1,930 words) - 11:41, 25 October 2013
Exeter/28 August 2013
! Part !! ompC (Part A) !! B0034 (Part B) !! AMP Plasmid !! RFP (Control) | AMP plasmid || 2155

3 KB (490 words) - 20:53, 2 October 2013
Team:Evry/Protocols/10
Transform the PTKred Plasmid<a href='http://www.addgene.org/41062/' target='_blank'>1Elimination of PTKred plasmid</h3>

3 KB (478 words) - 10:38, 6 September 2013
Exeter/30 August 2013
| AMP plasmid || 2155 K592011, B0015, AMP plasmid, and RFP were from the digestions on the 29th.

2 KB (301 words) - 20:39, 2 October 2013
Team:Washington/Outreach
... we gave them tape, which represents ligase, to complete their expression plasmid. </html> ... lysed the cell by opening the box so the student could extract both their plasmid and candy proteins to complete the protein expression process. 

3 KB (453 words) - 01:10, 27 September 2013
SUSTC/7 September 2013
...heir own biobrick of 1kbp, so it’s more convenient for us to collect the plasmid backbone.

357 B (66 words) - 06:19, 25 September 2013
Team:Washington/HOW TO LABEL EVERYTHING
Plasmid Name Plasmid Name

24 KB (2,801 words) - 23:47, 14 September 2013
Team:MIT/Notebook
<tr><td>8/13/2013</td><td>MK</td><td>Midiprep the 2xCr9-CMV_eYFP plasmid, Digest Goldengate products, Figure out what's going on with the 4xCr9-CMV_ Transfection of HEK193. Cas9-Venus fusions with target plasmid and guide RNA (CL)

33 KB (5,424 words) - 21:33, 28 October 2013
Team:UC Davis/Assembly
... by PCR, the full construct can be digested and inserted into the BioBrick plasmid of choice. It is, however, also possible to assemble through SOE PCR an ins ...with the antibiotic corresponding to the resistance provided by the second plasmid.

6 KB (873 words) - 18:36, 27 September 2013
Team:WHU-China/templates/standardpage doublepromoter
To exclude the problem of the divergence of plasmid copy number in different sample groups, which would make the result not tha We construct the report protein mCherry and EYFP on the same plasmid pSB1C3. When at detection, we set the fluorescence of EYFP as the control,

6 KB (1,001 words) - 21:02, 27 September 2013
Team:Paris Saclay/Notebook/August/22
===='''1 - Plasmid extraction of BBa_K1155000 from DH5α'''==== The extraction was good. We will digested the plasmid.

3 KB (475 words) - 01:16, 5 October 2013
Team:TU-Delft/Peptides
...roduction was driven by the strong T7 phage promoter. This gene containing plasmid was harboured in a BL21(DE3) strain that has lac promoter driven T7 polymer The presence of plasmid with gene inserts encoding the SUMO-peptide (<a href="http://parts.igem.org

19 KB (2,958 words) - 21:17, 4 October 2013
Team:RHIT/Protocol.html
2. Add 1-5 µL containing 1 pg-100 ng of plasmid DNA to the cell mixture. Carefully flick the tube 4-5 times to mix cells an ...nt of DNA. The segment of DNA created from ligation is called a plasmid. A plasmid is a circular piece of DNA.

259 KB (27,268 words) - 01:38, 28 September 2013
Team:Paris Bettencourt/Notebook/Trojan Horse/Wednesday 14th August.html
Purified Plasmid Purified Plasmid

13 KB (1,149 words) - 09:40, 3 October 2013
Team:Bielefeld-Germany/Collaborations
...moters/Catalog/Anderson Anderson promoter collection] J230100-J230119. The plasmid backbone of these BioBricks J61002 is very suitable for promoter insertion ...prisingly not fluoresce the strongest. This might be due to a problem with plasmid stability in this strain. Also, the unmeasured J23119 BioBrick seems to hol

12 KB (1,672 words) - 02:38, 29 October 2013
Team:Grenoble-EMSE-LSU/Project/KR
...ditional factor we had to take into account during the construction of the plasmid.

8 KB (1,102 words) - 17:22, 28 September 2013
Team:Tokyo Tech/Project/Ninja State Switching
...romoter. We introduced each plasmid into E. coli which has another plasmid to express LasR constitutively. Then, we added 3OC6HSL or 3OC12HSL to the ... We used Pcon-RBS-luxR-TT-Ptrc-RBS-tetR-TT as the regulator plasmid.

21 KB (3,651 words) - 23:06, 28 October 2013
Team:Tokyo Tech/Experiment/Crosstalk Confirmation Assay
...happen when we added intercellular molecules 3OC6HSL and 3OC12HSL to these plasmid sets. ...r. Making pairs from 2 regulators and 2 repressors, we had to prepare four plasmid sets. These sets are transformed into different cells (Gray KM et al., 1994

6 KB (1,065 words) - 02:59, 29 October 2013
Team:Tokyo Tech/Submitted Parts
... C6) because G2p (gene 2 protein) is an endonuclease which is needed for a plasmid to be replicated by M13 origin, and to be packaged into the phage particle. ...o different types of replication origins, M13 origin and pSB3 origin, this plasmid ([http://parts.igem.org/Part:BBa_K1139020 BBa_K1139020], Fig. 5-1-2) formed

9 KB (1,475 words) - 03:37, 29 October 2013
Team:BIOSINT Mexico/Protocols
'''Plasmid purification''' *(The eppendorf tube contains the purified plasmid)

9 KB (1,528 words) - 04:02, 28 September 2013
Team:Nanjing-China/tran
... the sole nitrogen source for the bacteria. A study has found that a large plasmid carries all the genes and complex pathways related to atrazine degradation ...Ps which is the negative control. "TRM+" is the experimental group where a plasmid-pGFP carrying TRM was transformed into Escherichia coli K-12. And the resul

8 KB (1,177 words) - 02:50, 28 September 2013
Team:ZJU-China/Notebook/LabNotes/June
|June 1||Gel electrophoresis and pick up several colonies. Small-scale plasmid purification and then cut with E+P. |June 6||Small-scale plasmid purification of 17F part. Sterilization of culture plates and cuvettes.

2 KB (274 words) - 09:48, 27 September 2013
SUSTC/14 September 2013
...ia grew up in LB. It’s so strange, and we started to suspect whether the plasmid has been transformed in. Since we had no time waiting to see the result of

1 KB (178 words) - 06:11, 25 September 2013
Team:USTC CHINA/Notebook/Protocols/Plasmid mini-prep
...ttps://2013.igem.org/Team:USTC_CHINA/Notebook/Protocols/Plasmid mini-prep">Plasmid mini-prep</a></div></div> <h1>Plasmid mini-prep</h1>

6 KB (834 words) - 16:50, 27 September 2013
Team:Tsinghua/Project-Sensor
Figure 6. Synthesized plasmid containing tVP16-LuxR-Plux-cyc100 mini promoter ...eng fragment was ligased with pRS423 fragment. We got the following Clone1 plasmid.

14 KB (1,965 words) - 01:55, 28 September 2013
Team:UniSalento Lecce/Overview
We cloned HpNikR from H.pylori strain G27 from the plasmid pET-NikR, sent us by prof. Alberto Danielli (from University of Bologna), t ...> G27 containing a divergent operon with two NikR-regulated promoters. The plasmid was sent us again by prof. Alberto Danielli as pNKTB. The promoters control

27 KB (3,989 words) - 14:58, 4 October 2013
Team:UChicago/Protocols
#resuspend plasmid DNA from kit plates #transform plasmid DNA into DH5a cells (why those cells?)

20 KB (3,354 words) - 21:58, 25 October 2013
Team:INSA Toulouse/contenu/project/e calculus design
.... Furthermore, we also imagined that switching DNA elements on a multicopy plasmid may not be completely wise, especially when our riboregulators were suppose ...d would be stable (no recombinases produced). Upon transformation with the plasmid containing the general inducer, input, output and carry modules, the E.

7 KB (1,004 words) - 20:40, 4 October 2013
Team:UniSalento Lecce/Data
...g HpNikR (from the genome of H. pylori G27) inserted into a pET-15b plasmid.<a name="o2_1"></a> For studying HpNikR, submitted as <a href="http://pa This part was amplified by PCR from the plasmid pETNikR. Primers used (including Biobrick Prefix and Suffix, lowercase)

32 KB (4,800 words) - 15:51, 4 October 2013
Team:SCUT/Team/Notebook
...L21) using MRS and LB medium. We also did transformation experiments using plasmid and the newly-preparation Top-10 competent cellS. .... To amplifiedα-Als gene,we did a PCR experiment.We did authentication of plasmid YEP352 by using enzyme E.coRⅠ. 

7 KB (1,214 words) - 20:28, 27 September 2013
Team:IIT Delhi/Protocol
Plasmid Isolation (Qiagen MiniPrep Plasmid Isolation Kit©) 1. Inoculate the cells containing the plasmid in 5ml LB tube and incubate overnight at 37⁰C. 

17 KB (2,496 words) - 04:17, 28 September 2013
Team:Duke/Project/Results/Part1
...rable. We then cloned a dCas9 plasmid without the fused mCherry gene; this plasmid was sequence confirmed but is yet to be integrated into yeast.

2 KB (312 words) - 23:24, 27 September 2013
Team:UC-Santa Cruz/Notebook3
5. Add 20ng of plasmid DNA so add 7ul of diluted plasmid DNA (PxYFPC-2) ...r the second plasmid DNA (PvCFPC-4) and negative control. Instead of using plasmid DNA add 7ul of sterile dH20 for negative

27 KB (4,367 words) - 04:04, 28 September 2013
Team:Hong Kong HKUST/characterization/ef1a
...rol was iDUET101a plasmid (<a href="http://www.addgene.org/17629/">Addgene Plasmid Number 17629</a>) that contains EGFP reporter driven by an EF-1alpha promot ...id contains EF-1alpha promoter and EGFP reporter. We have transfected this plasmid for positive control for EF-1alpha characterization.

16 KB (2,298 words) - 03:41, 29 October 2013
Team:NJU China/Data Page
... is better than plasmid, and the 467siRNA plasmid is better than 516 siRNA plasmid. In view of the results, we choose 467siRNA as our final targeting RNA.</br

39 KB (5,881 words) - 03:18, 29 October 2013
Team:NJU China/all
... is better than plasmid, and the 467siRNA plasmid is better than 516 siRNA plasmid. In view of the results, we choose 467siRNA as our final targeting RNA. 

17 KB (2,863 words) - 15:46, 27 September 2013
Team:TecMonterrey/final edge.js
...ic is found it’s critical to use an strategy to avoid this problem since plasmid loss will be reflected in loss of dosage of therapeutic proteins. A simple

118 KB (24,573 words) - 04:14, 28 September 2013
Team:Dundee/Project/MopQuantifying
...t the GST portion of the fusion protein could subsequently be removed. The plasmid expressing this fusion protein was called pGEX6P-1 PP1 CAT gamma and this w ..., the cultures were supplemented with 1mM IPTG to induce expression of the plasmid-encoded fusion protein (Fig 1A) and the cells were grown overnight at 20°C

9 KB (1,539 words) - 18:30, 28 October 2013
Team:Paris Bettencourt/Collaboration
...ell as the helper plasmid required for it to function. They sent us their plasmid pUC57amp-cI which is responsible for the repression of the holin and lysozy ...ell as the helper plasmid required for it to function. They sent us their plasmid pUC57amp-cI which is responsible for the repression of the holin and lysozy

14 KB (2,196 words) - 16:36, 5 December 2013